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Comparison of rapid diagnostic tests for the detection of Plasmodium vivax malaria in South Korea.

Kim JY, Ji SY, Goo YK, Na BK, Pyo HJ, Lee HN, Lee J, Kim NH, von Seidlein L, Cheng Q, Cho SH, Lee WJ - PLoS ONE (2013)

Bottom Line: The CareStart(TM) and SD Bioline had higher test sensitivities (99.4 and 98.8%, respectively) compared with the NanoSign and Asan Easy tests (93.0 and 94.7%, respectively).The quantitative accuracy of the four RDTs was also estimated by comparing results with P. vivax counts from blood samples.Lower test price would result in increased use of these RDTs in the field.

View Article: PubMed Central - PubMed

Affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong, Republic of Korea.

ABSTRACT
South Korea is one of many countries with endemic Plasmodium vivax malaria. Here we report the evaluation of four rapid diagnostic tests (RDTs) for diagnosis of this disease. A total of 253 subjects were enrolled in the study. The sensitivities, specificities and agreement frequencies were estimated by comparing the four RDTs against the standard of nested-PCR and microscopic examination. The CareStart(TM) and SD Bioline had higher test sensitivities (99.4 and 98.8%, respectively) compared with the NanoSign and Asan Easy tests (93.0 and 94.7%, respectively). The CareStart(TM) and SD Bioline tests could detect P. vivax in samples with parasite densities <150/μl, which was a slightly better performance than the other two RDTs. The quantitative accuracy of the four RDTs was also estimated by comparing results with P. vivax counts from blood samples. Lower test price would result in increased use of these RDTs in the field. The results of this study contribute valuable information that will aid in the selection of a diagnostic method for the detection of malaria.

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Related in: MedlinePlus

Sample selection profile for the evaluation of four rapid diagnostic tests.Microscopic examination, nested-PCR, and rapid diagnostic tests were performed.
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pone-0064353-g001: Sample selection profile for the evaluation of four rapid diagnostic tests.Microscopic examination, nested-PCR, and rapid diagnostic tests were performed.

Mentions: The study was conducted at the Division of Malaria and Parasitic Diseases, the Korea Centers for Disease Control and Prevention (KCDC), from June 2009–October 2011. Venous blood samples with EDTA were received from 171 patients diagnosed with vivax malaria at local health centers (Kang-wha, Paju, Gimpo, Yeon-Cheon; Fig. 1). Microscopic examination of Giemsa-stained thick and thin blood films was used to confirm the diagnosis. Samples were also received from 82 asymptomatic and aparasitemic healthy volunteers, which had been confirmed as P. vivax negative by microscopic examination and nested-PCR. Mean age of the patients was 47 years, and 75% of them were male. Blood specimens were kept cool and arrived at the KCDC within 24 hours after collection.


Comparison of rapid diagnostic tests for the detection of Plasmodium vivax malaria in South Korea.

Kim JY, Ji SY, Goo YK, Na BK, Pyo HJ, Lee HN, Lee J, Kim NH, von Seidlein L, Cheng Q, Cho SH, Lee WJ - PLoS ONE (2013)

Sample selection profile for the evaluation of four rapid diagnostic tests.Microscopic examination, nested-PCR, and rapid diagnostic tests were performed.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646812&req=5

pone-0064353-g001: Sample selection profile for the evaluation of four rapid diagnostic tests.Microscopic examination, nested-PCR, and rapid diagnostic tests were performed.
Mentions: The study was conducted at the Division of Malaria and Parasitic Diseases, the Korea Centers for Disease Control and Prevention (KCDC), from June 2009–October 2011. Venous blood samples with EDTA were received from 171 patients diagnosed with vivax malaria at local health centers (Kang-wha, Paju, Gimpo, Yeon-Cheon; Fig. 1). Microscopic examination of Giemsa-stained thick and thin blood films was used to confirm the diagnosis. Samples were also received from 82 asymptomatic and aparasitemic healthy volunteers, which had been confirmed as P. vivax negative by microscopic examination and nested-PCR. Mean age of the patients was 47 years, and 75% of them were male. Blood specimens were kept cool and arrived at the KCDC within 24 hours after collection.

Bottom Line: The CareStart(TM) and SD Bioline had higher test sensitivities (99.4 and 98.8%, respectively) compared with the NanoSign and Asan Easy tests (93.0 and 94.7%, respectively).The quantitative accuracy of the four RDTs was also estimated by comparing results with P. vivax counts from blood samples.Lower test price would result in increased use of these RDTs in the field.

View Article: PubMed Central - PubMed

Affiliation: Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong, Republic of Korea.

ABSTRACT
South Korea is one of many countries with endemic Plasmodium vivax malaria. Here we report the evaluation of four rapid diagnostic tests (RDTs) for diagnosis of this disease. A total of 253 subjects were enrolled in the study. The sensitivities, specificities and agreement frequencies were estimated by comparing the four RDTs against the standard of nested-PCR and microscopic examination. The CareStart(TM) and SD Bioline had higher test sensitivities (99.4 and 98.8%, respectively) compared with the NanoSign and Asan Easy tests (93.0 and 94.7%, respectively). The CareStart(TM) and SD Bioline tests could detect P. vivax in samples with parasite densities <150/μl, which was a slightly better performance than the other two RDTs. The quantitative accuracy of the four RDTs was also estimated by comparing results with P. vivax counts from blood samples. Lower test price would result in increased use of these RDTs in the field. The results of this study contribute valuable information that will aid in the selection of a diagnostic method for the detection of malaria.

Show MeSH
Related in: MedlinePlus