Limits...
Processing technology investigation of loquat (Eriobotrya japonica) leaf by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with chemometrics.

Wu L, Jiang X, Huang L, Chen S - PLoS ONE (2013)

Bottom Line: Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing.Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers.The method was further confirmed by a verification test with commercial PPY.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

ABSTRACT
Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and multivariate statistical analysis were used to investigate the processing technology of Loquat (Eriobotrya japonica) leaf (pipaye, PPY). The differences in samples processed using different methods were revealed by unsupervised principal component analysis (PCA). In the scores plot of PCA, honey-processed PPY (PPPY), crude PPY (CPPY), and heated PPY (HPPY) were clearly discriminated. Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing. Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers. The method was further confirmed by a verification test with commercial PPY. The orthogonal array experiment revealed an optimized processing condition with 50% honey at 140°C for 20 min after 4 h of moistening time, a process that provides significant information for standardized production.

Show MeSH

Related in: MedlinePlus

PCA (scores plot) of commercial CPPY and PPPY.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3646807&req=5

pone-0064178-g004: PCA (scores plot) of commercial CPPY and PPPY.

Mentions: In the verification test, CPPY and PPPY could be separated by the PCA score plot (Figure 4). This demonstrated that UPLC-QTOF-MS could be used as the method for identification between commercial CPPY and PPPY. Additionally, OPLS-DA was performed to generate an S-plot (Figure 3D). Two marker ions, marker a (0.95_221.0600/0.94_221.0670) and marker b (3.32_471.3449/3.17_471.3453), were successfully verified. Marker a could be detected in experimental and test PPPY samples, but could not be detected in CPPY (Figure 5A, 5B). The ion intensity of marker b in CPPY was higher than that in PPPY in experimental samples, and this was verified in test samples (Figure 5C, 5D). These results verified that markers a and b could be used as indicators in distinguishing between CPPY and PPPY.


Processing technology investigation of loquat (Eriobotrya japonica) leaf by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with chemometrics.

Wu L, Jiang X, Huang L, Chen S - PLoS ONE (2013)

PCA (scores plot) of commercial CPPY and PPPY.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646807&req=5

pone-0064178-g004: PCA (scores plot) of commercial CPPY and PPPY.
Mentions: In the verification test, CPPY and PPPY could be separated by the PCA score plot (Figure 4). This demonstrated that UPLC-QTOF-MS could be used as the method for identification between commercial CPPY and PPPY. Additionally, OPLS-DA was performed to generate an S-plot (Figure 3D). Two marker ions, marker a (0.95_221.0600/0.94_221.0670) and marker b (3.32_471.3449/3.17_471.3453), were successfully verified. Marker a could be detected in experimental and test PPPY samples, but could not be detected in CPPY (Figure 5A, 5B). The ion intensity of marker b in CPPY was higher than that in PPPY in experimental samples, and this was verified in test samples (Figure 5C, 5D). These results verified that markers a and b could be used as indicators in distinguishing between CPPY and PPPY.

Bottom Line: Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing.Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers.The method was further confirmed by a verification test with commercial PPY.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

ABSTRACT
Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and multivariate statistical analysis were used to investigate the processing technology of Loquat (Eriobotrya japonica) leaf (pipaye, PPY). The differences in samples processed using different methods were revealed by unsupervised principal component analysis (PCA). In the scores plot of PCA, honey-processed PPY (PPPY), crude PPY (CPPY), and heated PPY (HPPY) were clearly discriminated. Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing. Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers. The method was further confirmed by a verification test with commercial PPY. The orthogonal array experiment revealed an optimized processing condition with 50% honey at 140°C for 20 min after 4 h of moistening time, a process that provides significant information for standardized production.

Show MeSH
Related in: MedlinePlus