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Processing technology investigation of loquat (Eriobotrya japonica) leaf by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with chemometrics.

Wu L, Jiang X, Huang L, Chen S - PLoS ONE (2013)

Bottom Line: Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing.Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers.The method was further confirmed by a verification test with commercial PPY.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

ABSTRACT
Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and multivariate statistical analysis were used to investigate the processing technology of Loquat (Eriobotrya japonica) leaf (pipaye, PPY). The differences in samples processed using different methods were revealed by unsupervised principal component analysis (PCA). In the scores plot of PCA, honey-processed PPY (PPPY), crude PPY (CPPY), and heated PPY (HPPY) were clearly discriminated. Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing. Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers. The method was further confirmed by a verification test with commercial PPY. The orthogonal array experiment revealed an optimized processing condition with 50% honey at 140°C for 20 min after 4 h of moistening time, a process that provides significant information for standardized production.

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Related in: MedlinePlus

PCA (scores plot) of CPPY, HPPY, and PPPY.
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pone-0064178-g002: PCA (scores plot) of CPPY, HPPY, and PPPY.

Mentions: Figure 2 shows that CPPY, HPPY, and PPPY samples were divided into 3 main clusters observed in the PCA scores plot. Such division indicated that use of different processing methods could significantly alter the composition of compounds and that CPPY, HPPY, and PPPY were distinct from each other. This distinct separation could be representative of their multiple pharmacological effects.


Processing technology investigation of loquat (Eriobotrya japonica) leaf by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with chemometrics.

Wu L, Jiang X, Huang L, Chen S - PLoS ONE (2013)

PCA (scores plot) of CPPY, HPPY, and PPPY.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646807&req=5

pone-0064178-g002: PCA (scores plot) of CPPY, HPPY, and PPPY.
Mentions: Figure 2 shows that CPPY, HPPY, and PPPY samples were divided into 3 main clusters observed in the PCA scores plot. Such division indicated that use of different processing methods could significantly alter the composition of compounds and that CPPY, HPPY, and PPPY were distinct from each other. This distinct separation could be representative of their multiple pharmacological effects.

Bottom Line: Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing.Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers.The method was further confirmed by a verification test with commercial PPY.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

ABSTRACT
Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and multivariate statistical analysis were used to investigate the processing technology of Loquat (Eriobotrya japonica) leaf (pipaye, PPY). The differences in samples processed using different methods were revealed by unsupervised principal component analysis (PCA). In the scores plot of PCA, honey-processed PPY (PPPY), crude PPY (CPPY), and heated PPY (HPPY) were clearly discriminated. Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing. Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers. The method was further confirmed by a verification test with commercial PPY. The orthogonal array experiment revealed an optimized processing condition with 50% honey at 140°C for 20 min after 4 h of moistening time, a process that provides significant information for standardized production.

Show MeSH
Related in: MedlinePlus