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Processing technology investigation of loquat (Eriobotrya japonica) leaf by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with chemometrics.

Wu L, Jiang X, Huang L, Chen S - PLoS ONE (2013)

Bottom Line: Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing.Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers.The method was further confirmed by a verification test with commercial PPY.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

ABSTRACT
Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and multivariate statistical analysis were used to investigate the processing technology of Loquat (Eriobotrya japonica) leaf (pipaye, PPY). The differences in samples processed using different methods were revealed by unsupervised principal component analysis (PCA). In the scores plot of PCA, honey-processed PPY (PPPY), crude PPY (CPPY), and heated PPY (HPPY) were clearly discriminated. Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing. Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers. The method was further confirmed by a verification test with commercial PPY. The orthogonal array experiment revealed an optimized processing condition with 50% honey at 140°C for 20 min after 4 h of moistening time, a process that provides significant information for standardized production.

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Representative profiling of a PPY sample.
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pone-0064178-g001: Representative profiling of a PPY sample.

Mentions: Table 5 lists the tentatively identified compounds in CPPY, HPPY, and PPPY. A total of 15 compounds were identified by UPLC-QTOF/MS based on database interrogation, standard compounds, and references, as shown in Figure 1[29]–[34]. Peaks 10 and 11 were identified as OA and UA, respectively, based on retention times, MS, and MS/MS fragment ions [28], [33]. Peaks 1, 4, and 5 were identified as euscaphic acid, maslinic acid, and 2α-hydroxyursolic acid, respectively, based on retention times, and MS data for these peaks were consistent with references [29], [34]. Peaks 2, 3, 7, 9, and 12 were identified as 2α,19α-dihydroxyurs-3-oxo-urs-12-en-28-oic acid, 3-O-p-coumaroyltormentic acid, hyptadienic acid, 3β-O-coumaroyl-2α-hydroxy-urs-12-en-28-oic acid, and palmitic acid, respectively, based on molecular mass and MS/MS fragment ions [30], [32], [33]. Linolenic, linoleic, stearic, and isomeric stearic acids were identified according to molecular masses and fragment ions and have been identified in the seeds or fruits of E. japonica[31]. All other compounds have been previously reported to be present in the leaves of E. japonica.


Processing technology investigation of loquat (Eriobotrya japonica) leaf by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry combined with chemometrics.

Wu L, Jiang X, Huang L, Chen S - PLoS ONE (2013)

Representative profiling of a PPY sample.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3646807&req=5

pone-0064178-g001: Representative profiling of a PPY sample.
Mentions: Table 5 lists the tentatively identified compounds in CPPY, HPPY, and PPPY. A total of 15 compounds were identified by UPLC-QTOF/MS based on database interrogation, standard compounds, and references, as shown in Figure 1[29]–[34]. Peaks 10 and 11 were identified as OA and UA, respectively, based on retention times, MS, and MS/MS fragment ions [28], [33]. Peaks 1, 4, and 5 were identified as euscaphic acid, maslinic acid, and 2α-hydroxyursolic acid, respectively, based on retention times, and MS data for these peaks were consistent with references [29], [34]. Peaks 2, 3, 7, 9, and 12 were identified as 2α,19α-dihydroxyurs-3-oxo-urs-12-en-28-oic acid, 3-O-p-coumaroyltormentic acid, hyptadienic acid, 3β-O-coumaroyl-2α-hydroxy-urs-12-en-28-oic acid, and palmitic acid, respectively, based on molecular mass and MS/MS fragment ions [30], [32], [33]. Linolenic, linoleic, stearic, and isomeric stearic acids were identified according to molecular masses and fragment ions and have been identified in the seeds or fruits of E. japonica[31]. All other compounds have been previously reported to be present in the leaves of E. japonica.

Bottom Line: Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing.Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers.The method was further confirmed by a verification test with commercial PPY.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

ABSTRACT
Ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and multivariate statistical analysis were used to investigate the processing technology of Loquat (Eriobotrya japonica) leaf (pipaye, PPY). The differences in samples processed using different methods were revealed by unsupervised principal component analysis (PCA). In the scores plot of PCA, honey-processed PPY (PPPY), crude PPY (CPPY), and heated PPY (HPPY) were clearly discriminated. Furthermore, samples processed at different temperatures could also be distinguished; indeed, our PCA results demonstrated the importance of temperature during processing. Two unique marker ions were found to discriminate between PPPY and CPPY by orthogonal partial least squares discriminant analysis (OPLS-DA), which could be used as potential chemical markers. The method was further confirmed by a verification test with commercial PPY. The orthogonal array experiment revealed an optimized processing condition with 50% honey at 140°C for 20 min after 4 h of moistening time, a process that provides significant information for standardized production.

Show MeSH
Related in: MedlinePlus