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A chronic model of arthritis supported by a strain-specific periarticular lymph node in BALB/c mice.

Baddack U, Hartmann S, Bang H, Grobe J, Loddenkemper C, Lipp M, Müller G - Nat Commun (2013)

Bottom Line: However, BALB/c mice generate significantly higher titres of anticollagen and anticitrullinated peptide antibodies, show a stronger progressive joint destruction, and in the chronic phase the disease spreads between joints.Concomitant to the observation of a more severe pathology, we discovered a previously undescribed small periarticular lymph node in close proximity to the knee joint of BALB/c mice, which acts as the primary draining lymph node for the synovial cavity.Our model more closely reflects the pathology of rheumatoid arthritis than classical models of arthritis and is hence particularly suitable for further studies of disease pathogenesis.

View Article: PubMed Central - PubMed

Affiliation: Max-Delbrück-Center of Molecular Medicine, Department of Tumor Genetics and Immunogenetics, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.

ABSTRACT
Current animal models of arthritis only partially reflect the complexity of rheumatoid arthritis and typically lack either chronicity or autoantibody formation. Here we describe a model that combines features of antigen-induced arthritis and collagen-induced arthritis, which can be efficiently induced in BALB/c and C57BL/6 mice. However, BALB/c mice generate significantly higher titres of anticollagen and anticitrullinated peptide antibodies, show a stronger progressive joint destruction, and in the chronic phase the disease spreads between joints. Concomitant to the observation of a more severe pathology, we discovered a previously undescribed small periarticular lymph node in close proximity to the knee joint of BALB/c mice, which acts as the primary draining lymph node for the synovial cavity. Our model more closely reflects the pathology of rheumatoid arthritis than classical models of arthritis and is hence particularly suitable for further studies of disease pathogenesis.

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The paLN acts as the primary draining lymph node for the synovial cavity.Biotinylated splenocytes were injected intra-articularly into the knee joint of healthy BALB/c mice. Periarticular (left), popliteal (middle) or inguinal (right) lymph nodes were prepared 18 h (top) or 24 h (bottom) after injection. Splenocytes were visualized in red by staining with peroxidase-labelled streptavidin and AEC; counterstaining with hematoxylin is shown in blue. Sections are representative for three independent experiments. Black bars, 200 μm.
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f3: The paLN acts as the primary draining lymph node for the synovial cavity.Biotinylated splenocytes were injected intra-articularly into the knee joint of healthy BALB/c mice. Periarticular (left), popliteal (middle) or inguinal (right) lymph nodes were prepared 18 h (top) or 24 h (bottom) after injection. Splenocytes were visualized in red by staining with peroxidase-labelled streptavidin and AEC; counterstaining with hematoxylin is shown in blue. Sections are representative for three independent experiments. Black bars, 200 μm.

Mentions: Within 18 h, a large number of cells had migrated into the paLN, whereas no cells could be detected in the popliteal or inguinal lymph nodes (Fig. 3, top). However, after 24 h a very limited number of transferred splenocytes were found scattered in the popliteal lymph node (Fig. 3, bottom, black arrows). Thus, our data strongly implies that the paLN is the primary draining lymph node for the knee joint cavity.


A chronic model of arthritis supported by a strain-specific periarticular lymph node in BALB/c mice.

Baddack U, Hartmann S, Bang H, Grobe J, Loddenkemper C, Lipp M, Müller G - Nat Commun (2013)

The paLN acts as the primary draining lymph node for the synovial cavity.Biotinylated splenocytes were injected intra-articularly into the knee joint of healthy BALB/c mice. Periarticular (left), popliteal (middle) or inguinal (right) lymph nodes were prepared 18 h (top) or 24 h (bottom) after injection. Splenocytes were visualized in red by staining with peroxidase-labelled streptavidin and AEC; counterstaining with hematoxylin is shown in blue. Sections are representative for three independent experiments. Black bars, 200 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3644064&req=5

f3: The paLN acts as the primary draining lymph node for the synovial cavity.Biotinylated splenocytes were injected intra-articularly into the knee joint of healthy BALB/c mice. Periarticular (left), popliteal (middle) or inguinal (right) lymph nodes were prepared 18 h (top) or 24 h (bottom) after injection. Splenocytes were visualized in red by staining with peroxidase-labelled streptavidin and AEC; counterstaining with hematoxylin is shown in blue. Sections are representative for three independent experiments. Black bars, 200 μm.
Mentions: Within 18 h, a large number of cells had migrated into the paLN, whereas no cells could be detected in the popliteal or inguinal lymph nodes (Fig. 3, top). However, after 24 h a very limited number of transferred splenocytes were found scattered in the popliteal lymph node (Fig. 3, bottom, black arrows). Thus, our data strongly implies that the paLN is the primary draining lymph node for the knee joint cavity.

Bottom Line: However, BALB/c mice generate significantly higher titres of anticollagen and anticitrullinated peptide antibodies, show a stronger progressive joint destruction, and in the chronic phase the disease spreads between joints.Concomitant to the observation of a more severe pathology, we discovered a previously undescribed small periarticular lymph node in close proximity to the knee joint of BALB/c mice, which acts as the primary draining lymph node for the synovial cavity.Our model more closely reflects the pathology of rheumatoid arthritis than classical models of arthritis and is hence particularly suitable for further studies of disease pathogenesis.

View Article: PubMed Central - PubMed

Affiliation: Max-Delbrück-Center of Molecular Medicine, Department of Tumor Genetics and Immunogenetics, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.

ABSTRACT
Current animal models of arthritis only partially reflect the complexity of rheumatoid arthritis and typically lack either chronicity or autoantibody formation. Here we describe a model that combines features of antigen-induced arthritis and collagen-induced arthritis, which can be efficiently induced in BALB/c and C57BL/6 mice. However, BALB/c mice generate significantly higher titres of anticollagen and anticitrullinated peptide antibodies, show a stronger progressive joint destruction, and in the chronic phase the disease spreads between joints. Concomitant to the observation of a more severe pathology, we discovered a previously undescribed small periarticular lymph node in close proximity to the knee joint of BALB/c mice, which acts as the primary draining lymph node for the synovial cavity. Our model more closely reflects the pathology of rheumatoid arthritis than classical models of arthritis and is hence particularly suitable for further studies of disease pathogenesis.

Show MeSH
Related in: MedlinePlus