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Induction of spermatogenic synchrony by retinoic acid in neonatal mice.

Davis JC, Snyder EM, Hogarth CA, Small C, Griswold MD - Spermatogenesis (2013)

Bottom Line: The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood.In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood.The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Biosciences; Washington State University; Pullman, WA USA.

ABSTRACT
Retinoic acid (RA) is required for the successful differentiation and meiotic entry of germ cells in the murine testis. The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood. It has been shown that synchronous spermatogenesis can be induced in 2 d postpartum mice, but not in adult mice, by treating vitamin A sufficient males with RA. In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood. The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

No MeSH data available.


Related in: MedlinePlus

Figure 3. RA induces cell apoptosis in the neonatal male testis. The average number of TUNEL-positive cells per tubule after vehicle or RA treatment at each treatment age is shown. Filled bars represent data from vehicle treated animals and open bars represent data from RA treated animals. All error bars represent the standard error of the mean. *p < 0.05.
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Figure 3: Figure 3. RA induces cell apoptosis in the neonatal male testis. The average number of TUNEL-positive cells per tubule after vehicle or RA treatment at each treatment age is shown. Filled bars represent data from vehicle treated animals and open bars represent data from RA treated animals. All error bars represent the standard error of the mean. *p < 0.05.

Mentions: In our previous study we showed that when 2 dpp mice were treated with RA, there was a large increase in the numbers of apoptoic germ cells 48 h post-treatment.9 When testes of 4, 6 and 8 dpp RA-treated mice were analyzed for the presence of apoptotic cells by TUNEL 48 h post-treatment, we observed a similar increase in TUNEL-positive cells when compared with control for the 4 and 6 dpp samples. At 8 dpp, there was a general increase in apoptosis but the number of TUNEL-positive cells in the RA-treated samples was not significantly greater than those counted in the control samples (Fig. 3). Apoptosis in Sertoli cells was not observed at any of the ages examined.


Induction of spermatogenic synchrony by retinoic acid in neonatal mice.

Davis JC, Snyder EM, Hogarth CA, Small C, Griswold MD - Spermatogenesis (2013)

Figure 3. RA induces cell apoptosis in the neonatal male testis. The average number of TUNEL-positive cells per tubule after vehicle or RA treatment at each treatment age is shown. Filled bars represent data from vehicle treated animals and open bars represent data from RA treated animals. All error bars represent the standard error of the mean. *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3644044&req=5

Figure 3: Figure 3. RA induces cell apoptosis in the neonatal male testis. The average number of TUNEL-positive cells per tubule after vehicle or RA treatment at each treatment age is shown. Filled bars represent data from vehicle treated animals and open bars represent data from RA treated animals. All error bars represent the standard error of the mean. *p < 0.05.
Mentions: In our previous study we showed that when 2 dpp mice were treated with RA, there was a large increase in the numbers of apoptoic germ cells 48 h post-treatment.9 When testes of 4, 6 and 8 dpp RA-treated mice were analyzed for the presence of apoptotic cells by TUNEL 48 h post-treatment, we observed a similar increase in TUNEL-positive cells when compared with control for the 4 and 6 dpp samples. At 8 dpp, there was a general increase in apoptosis but the number of TUNEL-positive cells in the RA-treated samples was not significantly greater than those counted in the control samples (Fig. 3). Apoptosis in Sertoli cells was not observed at any of the ages examined.

Bottom Line: The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood.In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood.The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Biosciences; Washington State University; Pullman, WA USA.

ABSTRACT
Retinoic acid (RA) is required for the successful differentiation and meiotic entry of germ cells in the murine testis. The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood. It has been shown that synchronous spermatogenesis can be induced in 2 d postpartum mice, but not in adult mice, by treating vitamin A sufficient males with RA. In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood. The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

No MeSH data available.


Related in: MedlinePlus