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Induction of spermatogenic synchrony by retinoic acid in neonatal mice.

Davis JC, Snyder EM, Hogarth CA, Small C, Griswold MD - Spermatogenesis (2013)

Bottom Line: The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood.In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood.The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Biosciences; Washington State University; Pullman, WA USA.

ABSTRACT
Retinoic acid (RA) is required for the successful differentiation and meiotic entry of germ cells in the murine testis. The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood. It has been shown that synchronous spermatogenesis can be induced in 2 d postpartum mice, but not in adult mice, by treating vitamin A sufficient males with RA. In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood. The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

No MeSH data available.


Related in: MedlinePlus

Figure 2. Quantification of STRA8-immunopositive cells per tubule 24, 48 and 72 h post-RA injection at 4, 6 and 8 dpp, respectively. Filled bars represent data from vehicle treated animals and open bars represent data from RA treated animals. All error bars represent the standard error of the mean. * p < 0.05 ** p < 0.005
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Figure 2: Figure 2. Quantification of STRA8-immunopositive cells per tubule 24, 48 and 72 h post-RA injection at 4, 6 and 8 dpp, respectively. Filled bars represent data from vehicle treated animals and open bars represent data from RA treated animals. All error bars represent the standard error of the mean. * p < 0.05 ** p < 0.005

Mentions: The short-term effects of exogenous RA on germ cell development in this system was examined in testes from mice injected with RA at 4, 6 and 8 dpp and collected 24, 48 and 72 h post-treatment. Stimulated by retinoic acid gene 8 (Stra8) protein is a very sensitive and reliable marker for the short-term action of RA on germ cells and is required for the transition of Aal spermatogonia into A1 spermatogonia.12 At 24 h post-treatment, immunohistochemical detection of STRA8 revealed a significantly higher number of immunopositive cells per tubule in samples treated with RA when compared with vehicle-treated sections in each age group (Fig. 2). By 48 h after treatment there was very little difference in the number of cells positive for STRA8 when RA-treated samples were compared with vehicle controls for each age group. In contrast, by 72 h after treatment, the STRA8 immunopositive cells had nearly disappeared from the testes of the 4 dpp animal samples, were decreased by more than 50% in the testes of the 6 dpp samples and were unchanged in the testes of the 8 dpp samples. This relative number of STRA8 immunopositive cells at each age and time-point correlated with similar changes in the expression of Stra8 transcript, as detected via real time RT-PCR (data not shown).


Induction of spermatogenic synchrony by retinoic acid in neonatal mice.

Davis JC, Snyder EM, Hogarth CA, Small C, Griswold MD - Spermatogenesis (2013)

Figure 2. Quantification of STRA8-immunopositive cells per tubule 24, 48 and 72 h post-RA injection at 4, 6 and 8 dpp, respectively. Filled bars represent data from vehicle treated animals and open bars represent data from RA treated animals. All error bars represent the standard error of the mean. * p < 0.05 ** p < 0.005
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3644044&req=5

Figure 2: Figure 2. Quantification of STRA8-immunopositive cells per tubule 24, 48 and 72 h post-RA injection at 4, 6 and 8 dpp, respectively. Filled bars represent data from vehicle treated animals and open bars represent data from RA treated animals. All error bars represent the standard error of the mean. * p < 0.05 ** p < 0.005
Mentions: The short-term effects of exogenous RA on germ cell development in this system was examined in testes from mice injected with RA at 4, 6 and 8 dpp and collected 24, 48 and 72 h post-treatment. Stimulated by retinoic acid gene 8 (Stra8) protein is a very sensitive and reliable marker for the short-term action of RA on germ cells and is required for the transition of Aal spermatogonia into A1 spermatogonia.12 At 24 h post-treatment, immunohistochemical detection of STRA8 revealed a significantly higher number of immunopositive cells per tubule in samples treated with RA when compared with vehicle-treated sections in each age group (Fig. 2). By 48 h after treatment there was very little difference in the number of cells positive for STRA8 when RA-treated samples were compared with vehicle controls for each age group. In contrast, by 72 h after treatment, the STRA8 immunopositive cells had nearly disappeared from the testes of the 4 dpp animal samples, were decreased by more than 50% in the testes of the 6 dpp samples and were unchanged in the testes of the 8 dpp samples. This relative number of STRA8 immunopositive cells at each age and time-point correlated with similar changes in the expression of Stra8 transcript, as detected via real time RT-PCR (data not shown).

Bottom Line: The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood.In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood.The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Biosciences; Washington State University; Pullman, WA USA.

ABSTRACT
Retinoic acid (RA) is required for the successful differentiation and meiotic entry of germ cells in the murine testis. The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood. It has been shown that synchronous spermatogenesis can be induced in 2 d postpartum mice, but not in adult mice, by treating vitamin A sufficient males with RA. In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood. The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

No MeSH data available.


Related in: MedlinePlus