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Induction of spermatogenic synchrony by retinoic acid in neonatal mice.

Davis JC, Snyder EM, Hogarth CA, Small C, Griswold MD - Spermatogenesis (2013)

Bottom Line: The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood.In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood.The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Biosciences; Washington State University; Pullman, WA USA.

ABSTRACT
Retinoic acid (RA) is required for the successful differentiation and meiotic entry of germ cells in the murine testis. The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood. It has been shown that synchronous spermatogenesis can be induced in 2 d postpartum mice, but not in adult mice, by treating vitamin A sufficient males with RA. In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood. The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

No MeSH data available.


Related in: MedlinePlus

Figure 1. Degrees of synchrony resulting from RA treatment of mice at different neonatal ages. (A–D) Representative cross sections of an adult male testis after vehicle treatment (A), RA treatment at 4 dpp (B), 6 dpp (C) and 8 dpp (D) followed by recovery into adulthood. (E–H) Graphical representation of all 12 stages in cross sections of the seminiferous epithelium after vehicle treatment (E), RA treatment at 4 (F), 6 (G), and 8 (H) dpp and recovery to 65 dpp. (I–K) Resulting synchrony factors with treatment of vehicle or RA at 4 (I), 6 (J), and 8 (K) dpp. * p < 0.05. ** p < 0.005. All error bars represent standard error of the mean. Scale bars represent 100 µm.
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Figure 1: Figure 1. Degrees of synchrony resulting from RA treatment of mice at different neonatal ages. (A–D) Representative cross sections of an adult male testis after vehicle treatment (A), RA treatment at 4 dpp (B), 6 dpp (C) and 8 dpp (D) followed by recovery into adulthood. (E–H) Graphical representation of all 12 stages in cross sections of the seminiferous epithelium after vehicle treatment (E), RA treatment at 4 (F), 6 (G), and 8 (H) dpp and recovery to 65 dpp. (I–K) Resulting synchrony factors with treatment of vehicle or RA at 4 (I), 6 (J), and 8 (K) dpp. * p < 0.05. ** p < 0.005. All error bars represent standard error of the mean. Scale bars represent 100 µm.

Mentions: To determine the age at which RA treatment no longer results in synchronous spermatogenesis, murine male pups at 4, 6 and 8 dpp were treated with a single injection of RA and then maintained until they were 65 d of age (Fig. 1). At least 200 tubules from each treated animal were staged and are shown as a fraction of the total stages represented in each sample. In addition, the average synchrony factors were calculated for each sample.10 The synchrony factor is expressed as a fraction of the cycle of the seminiferous epithelium, and is not influenced by the differing duration of the stages of this cycle.


Induction of spermatogenic synchrony by retinoic acid in neonatal mice.

Davis JC, Snyder EM, Hogarth CA, Small C, Griswold MD - Spermatogenesis (2013)

Figure 1. Degrees of synchrony resulting from RA treatment of mice at different neonatal ages. (A–D) Representative cross sections of an adult male testis after vehicle treatment (A), RA treatment at 4 dpp (B), 6 dpp (C) and 8 dpp (D) followed by recovery into adulthood. (E–H) Graphical representation of all 12 stages in cross sections of the seminiferous epithelium after vehicle treatment (E), RA treatment at 4 (F), 6 (G), and 8 (H) dpp and recovery to 65 dpp. (I–K) Resulting synchrony factors with treatment of vehicle or RA at 4 (I), 6 (J), and 8 (K) dpp. * p < 0.05. ** p < 0.005. All error bars represent standard error of the mean. Scale bars represent 100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3644044&req=5

Figure 1: Figure 1. Degrees of synchrony resulting from RA treatment of mice at different neonatal ages. (A–D) Representative cross sections of an adult male testis after vehicle treatment (A), RA treatment at 4 dpp (B), 6 dpp (C) and 8 dpp (D) followed by recovery into adulthood. (E–H) Graphical representation of all 12 stages in cross sections of the seminiferous epithelium after vehicle treatment (E), RA treatment at 4 (F), 6 (G), and 8 (H) dpp and recovery to 65 dpp. (I–K) Resulting synchrony factors with treatment of vehicle or RA at 4 (I), 6 (J), and 8 (K) dpp. * p < 0.05. ** p < 0.005. All error bars represent standard error of the mean. Scale bars represent 100 µm.
Mentions: To determine the age at which RA treatment no longer results in synchronous spermatogenesis, murine male pups at 4, 6 and 8 dpp were treated with a single injection of RA and then maintained until they were 65 d of age (Fig. 1). At least 200 tubules from each treated animal were staged and are shown as a fraction of the total stages represented in each sample. In addition, the average synchrony factors were calculated for each sample.10 The synchrony factor is expressed as a fraction of the cycle of the seminiferous epithelium, and is not influenced by the differing duration of the stages of this cycle.

Bottom Line: The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood.In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood.The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

View Article: PubMed Central - PubMed

Affiliation: School of Molecular Biosciences; Washington State University; Pullman, WA USA.

ABSTRACT
Retinoic acid (RA) is required for the successful differentiation and meiotic entry of germ cells in the murine testis. The availability of RA to undifferentiated germ cells begins in a variable, uneven pattern during the first few days after birth and establishes the asynchronous pattern of germ cell differentiation in adulthood. It has been shown that synchronous spermatogenesis can be induced in 2 d postpartum mice, but not in adult mice, by treating vitamin A sufficient males with RA. In this study, neonatal males were treated at different ages with a single dose of RA and spermatogenesis was examined after recovery to adulthood. The failure of exogenous RA to alter asynchrony correlates with the appearance of meiotic preleptotene spermatocytes within the seminiferous epithelium.

No MeSH data available.


Related in: MedlinePlus