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An androgenic agricultural contaminant impairs female reproductive behaviour in a freshwater fish.

Saaristo M, Tomkins P, Allinson M, Allinson G, Wong BB - PLoS ONE (2013)

Bottom Line: We found that females exposed to trenbolone approached males less and spent more time swimming away from males than non-exposed (control) females.By contrast, we found no difference in the behaviour of exposed and non-exposed males.Our study illustrates how anthropogenic contaminants can have sex-specific effects, and highlights the need to examine the behavioural responses of environmental contaminants in both sexes.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, Monash University, Melbourne, Victoria, Australia. minna.saaristo@monash.edu

ABSTRACT
Endocrine disrupting chemicals (EDCs) are a large group of environmental pollutants that can interfere with the endocrine system function of organisms at very low levels. One compound of great concern is trenbolone, which is widely used as a growth promoter in the cattle industry in many parts of the world. The aim of this study was to test how short-term (21-day) exposure to an environmentally relevant concentration of 17β-trenbolone (measured concentration 6 ng/L) affects reproductive behaviour and fin morphology in the eastern mosquitofish (Gambusia holbrooki). The mosquitofish is a sexually dimorphic livebearer with males inseminating females using their modified anal fin, the gonopodium, as an intromittent organ. Although the species has a coercive mating system, females are able to exert some control over the success of male mating attempts by selectively associating with, or avoiding, certain males over others. We found that females exposed to trenbolone approached males less and spent more time swimming away from males than non-exposed (control) females. By contrast, we found no difference in the behaviour of exposed and non-exposed males. Furthermore, exposure did not affect the anal fin morphology of males or females. This is the first study to demonstrate that exposure to an androgenic EDC can impair female (but not male) behaviour. Our study illustrates how anthropogenic contaminants can have sex-specific effects, and highlights the need to examine the behavioural responses of environmental contaminants in both sexes.

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Related in: MedlinePlus

Mean percentage (± SE) of (A) time and (B) frequency females spent swimming away from the male during each trial.The two treatments are: Control = fish exposed to freshwater (n = 18), and Trenbolone = fish exposed to 6 ng/L of 17β-trenbolone (n = 19); Asterisk indicates a significant difference (p<0.05) between the TB treatment and control.
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pone-0062782-g002: Mean percentage (± SE) of (A) time and (B) frequency females spent swimming away from the male during each trial.The two treatments are: Control = fish exposed to freshwater (n = 18), and Trenbolone = fish exposed to 6 ng/L of 17β-trenbolone (n = 19); Asterisk indicates a significant difference (p<0.05) between the TB treatment and control.

Mentions: Trenbolone-exposed females spent less time associating with the males (duration of time (ms): Mann-Whitney: U = 106.000, p = 0.048, n = 37; number of times: U = 102.000, p = 0.035, n = 37; Fig. 1a,b). Females, instead, spent more time swimming away from the males (duration of time (ms): Mann-Whitney: U = 102.000, p = 0.036, n = 37; Fig. 2a), although the frequency of this behaviour did not differ between the treatments (Mann-Whitney: U = 127.000, p = 0.181, n = 37; Fig. 2b). Aggressive behaviours were not significantly affected (all p>0.05).


An androgenic agricultural contaminant impairs female reproductive behaviour in a freshwater fish.

Saaristo M, Tomkins P, Allinson M, Allinson G, Wong BB - PLoS ONE (2013)

Mean percentage (± SE) of (A) time and (B) frequency females spent swimming away from the male during each trial.The two treatments are: Control = fish exposed to freshwater (n = 18), and Trenbolone = fish exposed to 6 ng/L of 17β-trenbolone (n = 19); Asterisk indicates a significant difference (p<0.05) between the TB treatment and control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3643955&req=5

pone-0062782-g002: Mean percentage (± SE) of (A) time and (B) frequency females spent swimming away from the male during each trial.The two treatments are: Control = fish exposed to freshwater (n = 18), and Trenbolone = fish exposed to 6 ng/L of 17β-trenbolone (n = 19); Asterisk indicates a significant difference (p<0.05) between the TB treatment and control.
Mentions: Trenbolone-exposed females spent less time associating with the males (duration of time (ms): Mann-Whitney: U = 106.000, p = 0.048, n = 37; number of times: U = 102.000, p = 0.035, n = 37; Fig. 1a,b). Females, instead, spent more time swimming away from the males (duration of time (ms): Mann-Whitney: U = 102.000, p = 0.036, n = 37; Fig. 2a), although the frequency of this behaviour did not differ between the treatments (Mann-Whitney: U = 127.000, p = 0.181, n = 37; Fig. 2b). Aggressive behaviours were not significantly affected (all p>0.05).

Bottom Line: We found that females exposed to trenbolone approached males less and spent more time swimming away from males than non-exposed (control) females.By contrast, we found no difference in the behaviour of exposed and non-exposed males.Our study illustrates how anthropogenic contaminants can have sex-specific effects, and highlights the need to examine the behavioural responses of environmental contaminants in both sexes.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, Monash University, Melbourne, Victoria, Australia. minna.saaristo@monash.edu

ABSTRACT
Endocrine disrupting chemicals (EDCs) are a large group of environmental pollutants that can interfere with the endocrine system function of organisms at very low levels. One compound of great concern is trenbolone, which is widely used as a growth promoter in the cattle industry in many parts of the world. The aim of this study was to test how short-term (21-day) exposure to an environmentally relevant concentration of 17β-trenbolone (measured concentration 6 ng/L) affects reproductive behaviour and fin morphology in the eastern mosquitofish (Gambusia holbrooki). The mosquitofish is a sexually dimorphic livebearer with males inseminating females using their modified anal fin, the gonopodium, as an intromittent organ. Although the species has a coercive mating system, females are able to exert some control over the success of male mating attempts by selectively associating with, or avoiding, certain males over others. We found that females exposed to trenbolone approached males less and spent more time swimming away from males than non-exposed (control) females. By contrast, we found no difference in the behaviour of exposed and non-exposed males. Furthermore, exposure did not affect the anal fin morphology of males or females. This is the first study to demonstrate that exposure to an androgenic EDC can impair female (but not male) behaviour. Our study illustrates how anthropogenic contaminants can have sex-specific effects, and highlights the need to examine the behavioural responses of environmental contaminants in both sexes.

Show MeSH
Related in: MedlinePlus