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The protective effects of beta-casomorphin-7 against glucose -induced renal oxidative stress in vivo and vitro.

Zhang W, Miao J, Wang S, Zhang Y - PLoS ONE (2013)

Bottom Line: Furthermore, BCM7 alleviated high glucose-induced decreasement in SOD and GPx activity, increasement in MDA contents in the NRK-52E cells.Moreover losartan (antagonist of angiotensin II type I receptor) lowered the high glucose-induced oxidative stress in the NRK-52E cells.Our results suggest that administration of BCM7 would alleviate high glucose-induced renal oxidative stress in vivo and in vitro, which may be associated with down regulation of the concentration of Ang II partly.

View Article: PubMed Central - PubMed

Affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agriculture University, Nanjing, People's Republic of China.

ABSTRACT
Oxidative stress is implicated in the pathogenesis of diabetic nephropathy. The present study aimed to investigate the effect of β-casomorphin-7 (BCM7) on the oxidative stress occurring in kidney tissue in streptozotocin (STZ)-induced diabetic rats and proximal tubular epithelial cells (NRK-52E) exposure to high glucose (HG) by using biochemical methods. There is a significant decrease in plasma insulin and a significant increase in plasma glucagon in the rats of diabetic group. Oral administration of BCM7 for 30 days to rats with STZ-induced diabetes resulted in a significant increase in serum level of insulin, and a decrease in the level of glucagon. Moreover, rats with STZ-induced diabetes had lower levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidative capacity (T-AOC), higher levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in the kidney than that in the control rats. The administration of BCM7 altered the changes of SOD, GPx, T-AOC, MDA and H2O2 in the kidney of diabetic rats. Furthermore, BCM7 alleviated high glucose-induced decreasement in SOD and GPx activity, increasement in MDA contents in the NRK-52E cells. BCM7 ameliorated the changes of angiotensin converting enzyme (ACE) and ACE2 levels in the kidney of diabetic rats and BCM7 lowered the levels of angiotensin (Ang)II in the kidney of diabetic rats and culture medium for cells. Moreover losartan (antagonist of angiotensin II type I receptor) lowered the high glucose-induced oxidative stress in the NRK-52E cells. Our results suggest that administration of BCM7 would alleviate high glucose-induced renal oxidative stress in vivo and in vitro, which may be associated with down regulation of the concentration of Ang II partly.

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Changes of ACE2 and ACE in the kidney (n = 8).Data are expressed as mean±SEM (n = 8). (C) Control group; (D) Diabetic group; (BCM7) β-casomorphin-7 treatment group. *P<0.05, comparison between C group and D group. #P<0.05, comparison between D group and BCM7.
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pone-0063472-g008: Changes of ACE2 and ACE in the kidney (n = 8).Data are expressed as mean±SEM (n = 8). (C) Control group; (D) Diabetic group; (BCM7) β-casomorphin-7 treatment group. *P<0.05, comparison between C group and D group. #P<0.05, comparison between D group and BCM7.

Mentions: Real-time PCR revealed that compared to the rats of the control group, the diabetic rats had elevated expression of ACE mRNA and decreased expression of ACE2 mRNA. The expression of ACE mRNA was attenuated and ACE2 mRNA was increased by BCM7 treatment (Fig. 8).


The protective effects of beta-casomorphin-7 against glucose -induced renal oxidative stress in vivo and vitro.

Zhang W, Miao J, Wang S, Zhang Y - PLoS ONE (2013)

Changes of ACE2 and ACE in the kidney (n = 8).Data are expressed as mean±SEM (n = 8). (C) Control group; (D) Diabetic group; (BCM7) β-casomorphin-7 treatment group. *P<0.05, comparison between C group and D group. #P<0.05, comparison between D group and BCM7.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3643933&req=5

pone-0063472-g008: Changes of ACE2 and ACE in the kidney (n = 8).Data are expressed as mean±SEM (n = 8). (C) Control group; (D) Diabetic group; (BCM7) β-casomorphin-7 treatment group. *P<0.05, comparison between C group and D group. #P<0.05, comparison between D group and BCM7.
Mentions: Real-time PCR revealed that compared to the rats of the control group, the diabetic rats had elevated expression of ACE mRNA and decreased expression of ACE2 mRNA. The expression of ACE mRNA was attenuated and ACE2 mRNA was increased by BCM7 treatment (Fig. 8).

Bottom Line: Furthermore, BCM7 alleviated high glucose-induced decreasement in SOD and GPx activity, increasement in MDA contents in the NRK-52E cells.Moreover losartan (antagonist of angiotensin II type I receptor) lowered the high glucose-induced oxidative stress in the NRK-52E cells.Our results suggest that administration of BCM7 would alleviate high glucose-induced renal oxidative stress in vivo and in vitro, which may be associated with down regulation of the concentration of Ang II partly.

View Article: PubMed Central - PubMed

Affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agriculture University, Nanjing, People's Republic of China.

ABSTRACT
Oxidative stress is implicated in the pathogenesis of diabetic nephropathy. The present study aimed to investigate the effect of β-casomorphin-7 (BCM7) on the oxidative stress occurring in kidney tissue in streptozotocin (STZ)-induced diabetic rats and proximal tubular epithelial cells (NRK-52E) exposure to high glucose (HG) by using biochemical methods. There is a significant decrease in plasma insulin and a significant increase in plasma glucagon in the rats of diabetic group. Oral administration of BCM7 for 30 days to rats with STZ-induced diabetes resulted in a significant increase in serum level of insulin, and a decrease in the level of glucagon. Moreover, rats with STZ-induced diabetes had lower levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidative capacity (T-AOC), higher levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in the kidney than that in the control rats. The administration of BCM7 altered the changes of SOD, GPx, T-AOC, MDA and H2O2 in the kidney of diabetic rats. Furthermore, BCM7 alleviated high glucose-induced decreasement in SOD and GPx activity, increasement in MDA contents in the NRK-52E cells. BCM7 ameliorated the changes of angiotensin converting enzyme (ACE) and ACE2 levels in the kidney of diabetic rats and BCM7 lowered the levels of angiotensin (Ang)II in the kidney of diabetic rats and culture medium for cells. Moreover losartan (antagonist of angiotensin II type I receptor) lowered the high glucose-induced oxidative stress in the NRK-52E cells. Our results suggest that administration of BCM7 would alleviate high glucose-induced renal oxidative stress in vivo and in vitro, which may be associated with down regulation of the concentration of Ang II partly.

Show MeSH
Related in: MedlinePlus