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Euphorbia tirucalli L.-comprehensive characterization of a drought tolerant plant with a potential as biofuel source.

Hastilestari BR, Mudersbach M, Tomala F, Vogt H, Biskupek-Korell B, Van Damme P, Guretzki S, Papenbrock J - PLoS ONE (2013)

Bottom Line: Of late, decrease in mineral oil supplies has stimulated research on use of biomass as an alternative energy source.Climate change has brought problems such as increased drought and erratic rains.Malate measurement indicates that there is induction of CAM in leaves following drought stress.

View Article: PubMed Central - PubMed

Affiliation: Institute of Botany, Gottfried Wilhelm Leibniz University Hannover, Hannover, Germany.

ABSTRACT
Of late, decrease in mineral oil supplies has stimulated research on use of biomass as an alternative energy source. Climate change has brought problems such as increased drought and erratic rains. This, together with a rise in land degeneration problems with concomitant loss in soil fertility has inspired the scientific world to look for alternative bio-energy species. Euphorbia tirucalli L., a tree with C3/CAM metabolism in leaves/stem, can be cultivated on marginal, arid land and could be a good alternative source of biofuel. We analyzed a broad variety of E. tirucalli plants collected from different countries for their genetic diversity using AFLP. Physiological responses to induced drought stress were determined in a number of genotypes by monitoring growth parameters and influence on photosynthesis. For future breeding of economically interesting genotypes, rubber content and biogas production were quantified. Cluster analysis shows that the studied genotypes are divided into two groups, African and mostly non-African genotypes. Different genotypes respond significantly different to various levels of water. Malate measurement indicates that there is induction of CAM in leaves following drought stress. Rubber content varies strongly between genotypes. An investigation of the biogas production capacities of six E. tirucalli genotypes reveals biogas yields higher than from rapeseed but lower than maize silage.

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Related in: MedlinePlus

Dendrogram of twelve E.tirucalli genotypes calculated with UPGMA showing the phenetic relationships within the colletion.Bootstrap values≥50% are above the branches.
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pone-0063501-g001: Dendrogram of twelve E.tirucalli genotypes calculated with UPGMA showing the phenetic relationships within the colletion.Bootstrap values≥50% are above the branches.

Mentions: AFLP technique was used as a tool for assessing species relationships within the E. tirucalli collection. Seven primer combinations were selected for AFLP analysis (Table 1). Total number of polymorphic bands was 243 with a mean of 34.7. We were able to derive two main groups from the phylogenetic analysis of the 12 accessions of E. tirucalli cluster analysis using UPGMA with 1000 bootstrap replicates (Fig. 1). Nevertheless, the genotypes tested share a lot of similarities as evidenced from the low bootstrap values. The first group consists of two clades and comprises mainly genotypes from Africa: Burundi, Morocco, Senegal and Togo accessions that are clustered with a bootstrap value of 63. The second group consists of four clades with mainly non-African genotypes (except Kenya and Rwanda): Ajmer (India), Hannover (Germany), Indonesia, Italy, Jaipur (India), Kenya, Rwanda and USA with a bootstrap value of 72. A dendrogram derived from NJ calculation showed the same pattern (data not shown). All genotypes have been propagated by cuttings and cultivated in the greenhouse since a long time or at least for a couple of years. Therefore they should have the same amount of endophytes, if any. In our AFLP analysis the genotypes differ in several hundred bands. In case there are some bands originating from endophytes they would not influence the results significantly.


Euphorbia tirucalli L.-comprehensive characterization of a drought tolerant plant with a potential as biofuel source.

Hastilestari BR, Mudersbach M, Tomala F, Vogt H, Biskupek-Korell B, Van Damme P, Guretzki S, Papenbrock J - PLoS ONE (2013)

Dendrogram of twelve E.tirucalli genotypes calculated with UPGMA showing the phenetic relationships within the colletion.Bootstrap values≥50% are above the branches.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3643915&req=5

pone-0063501-g001: Dendrogram of twelve E.tirucalli genotypes calculated with UPGMA showing the phenetic relationships within the colletion.Bootstrap values≥50% are above the branches.
Mentions: AFLP technique was used as a tool for assessing species relationships within the E. tirucalli collection. Seven primer combinations were selected for AFLP analysis (Table 1). Total number of polymorphic bands was 243 with a mean of 34.7. We were able to derive two main groups from the phylogenetic analysis of the 12 accessions of E. tirucalli cluster analysis using UPGMA with 1000 bootstrap replicates (Fig. 1). Nevertheless, the genotypes tested share a lot of similarities as evidenced from the low bootstrap values. The first group consists of two clades and comprises mainly genotypes from Africa: Burundi, Morocco, Senegal and Togo accessions that are clustered with a bootstrap value of 63. The second group consists of four clades with mainly non-African genotypes (except Kenya and Rwanda): Ajmer (India), Hannover (Germany), Indonesia, Italy, Jaipur (India), Kenya, Rwanda and USA with a bootstrap value of 72. A dendrogram derived from NJ calculation showed the same pattern (data not shown). All genotypes have been propagated by cuttings and cultivated in the greenhouse since a long time or at least for a couple of years. Therefore they should have the same amount of endophytes, if any. In our AFLP analysis the genotypes differ in several hundred bands. In case there are some bands originating from endophytes they would not influence the results significantly.

Bottom Line: Of late, decrease in mineral oil supplies has stimulated research on use of biomass as an alternative energy source.Climate change has brought problems such as increased drought and erratic rains.Malate measurement indicates that there is induction of CAM in leaves following drought stress.

View Article: PubMed Central - PubMed

Affiliation: Institute of Botany, Gottfried Wilhelm Leibniz University Hannover, Hannover, Germany.

ABSTRACT
Of late, decrease in mineral oil supplies has stimulated research on use of biomass as an alternative energy source. Climate change has brought problems such as increased drought and erratic rains. This, together with a rise in land degeneration problems with concomitant loss in soil fertility has inspired the scientific world to look for alternative bio-energy species. Euphorbia tirucalli L., a tree with C3/CAM metabolism in leaves/stem, can be cultivated on marginal, arid land and could be a good alternative source of biofuel. We analyzed a broad variety of E. tirucalli plants collected from different countries for their genetic diversity using AFLP. Physiological responses to induced drought stress were determined in a number of genotypes by monitoring growth parameters and influence on photosynthesis. For future breeding of economically interesting genotypes, rubber content and biogas production were quantified. Cluster analysis shows that the studied genotypes are divided into two groups, African and mostly non-African genotypes. Different genotypes respond significantly different to various levels of water. Malate measurement indicates that there is induction of CAM in leaves following drought stress. Rubber content varies strongly between genotypes. An investigation of the biogas production capacities of six E. tirucalli genotypes reveals biogas yields higher than from rapeseed but lower than maize silage.

Show MeSH
Related in: MedlinePlus