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Characterization of gonadal transcriptomes from Nile tilapia (Oreochromis niloticus) reveals differentially expressed genes.

Tao W, Yuan J, Zhou L, Sun L, Sun Y, Yang S, Li M, Zeng S, Huang B, Wang D - PLoS ONE (2013)

Bottom Line: Of these, 259 genes were found to be specifically expressed in XY gonads, and 69 were found to be specific to XX gonads.Totally, 187 XX- and 1,358 XY-enhanced genes were identified, and 2,978 genes were found to be co-expressed in XX and XY gonads.Both estrogen and androgen receptors were found to be expressed in XX gonads, but only estrogen receptors were expressed in XY gonads at 5 dah.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing, PR China.

ABSTRACT
Four pairs of XX and XY gonads from Nile tilapia were sequenced at four developmental stages, 5, 30, 90, and 180 days after hatching (dah) using Illumina Hiseq(TM) technology. This produced 28 Gb sequences, which were mapped to 21,334 genes. Of these, 259 genes were found to be specifically expressed in XY gonads, and 69 were found to be specific to XX gonads. Totally, 187 XX- and 1,358 XY-enhanced genes were identified, and 2,978 genes were found to be co-expressed in XX and XY gonads. Almost all steroidogenic enzymes, including cyp19a1a, were up-regulated in XX gonads at 5 dah; but in XY gonads these enzymes, including cyp11b2, were significantly up-regulated at 90 dah, indicating that, at a time critical to sex determination, the XX fish produced estrogen and the XY fish did not produce androgens. The most pronounced expression of steroidogenic enzyme genes was observed at 30 and 90 dah for XX and XY gonads, corresponding to the initiation of germ cell meiosis in the female and male gonads, respectively. Both estrogen and androgen receptors were found to be expressed in XX gonads, but only estrogen receptors were expressed in XY gonads at 5 dah. This could explain why exogenous steroid treatment induced XX and XY sex reversal. The XX-enhanced expression of cyp19a1a and cyp19a1b at all stages suggests an important role for estrogen in female sex determination and maintenance of phenotypic sex. This work is the largest collection of gonadal transcriptome data in tilapia and lays the foundation for future studies into the molecular mechanisms of sex determination and maintenance of phenotypic sex in non-model teleosts.

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Venn diagram showing the number of genes expressed in XX (21,075) and XY (21,265) gonads throughout at the four stages of development.A total of 21,006 genes were expressed in both XX and XY gonads, but 69 and 259 genes were specifically expressed in XX and XY gonads, respectively.
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pone-0063604-g002: Venn diagram showing the number of genes expressed in XX (21,075) and XY (21,265) gonads throughout at the four stages of development.A total of 21,006 genes were expressed in both XX and XY gonads, but 69 and 259 genes were specifically expressed in XX and XY gonads, respectively.

Mentions: Of the 21,334 genes from eight transcriptomes, 21,006 were found to be co-expressed in both XX and XY gonads, and 69 and 259 genes were found to be expressed exclusively in XX and XY gonads, respectively (Figure 2). The number of XY-SEGs from all four developmental stages was nearly four-fold greater than for XX-SEGs.


Characterization of gonadal transcriptomes from Nile tilapia (Oreochromis niloticus) reveals differentially expressed genes.

Tao W, Yuan J, Zhou L, Sun L, Sun Y, Yang S, Li M, Zeng S, Huang B, Wang D - PLoS ONE (2013)

Venn diagram showing the number of genes expressed in XX (21,075) and XY (21,265) gonads throughout at the four stages of development.A total of 21,006 genes were expressed in both XX and XY gonads, but 69 and 259 genes were specifically expressed in XX and XY gonads, respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3643912&req=5

pone-0063604-g002: Venn diagram showing the number of genes expressed in XX (21,075) and XY (21,265) gonads throughout at the four stages of development.A total of 21,006 genes were expressed in both XX and XY gonads, but 69 and 259 genes were specifically expressed in XX and XY gonads, respectively.
Mentions: Of the 21,334 genes from eight transcriptomes, 21,006 were found to be co-expressed in both XX and XY gonads, and 69 and 259 genes were found to be expressed exclusively in XX and XY gonads, respectively (Figure 2). The number of XY-SEGs from all four developmental stages was nearly four-fold greater than for XX-SEGs.

Bottom Line: Of these, 259 genes were found to be specifically expressed in XY gonads, and 69 were found to be specific to XX gonads.Totally, 187 XX- and 1,358 XY-enhanced genes were identified, and 2,978 genes were found to be co-expressed in XX and XY gonads.Both estrogen and androgen receptors were found to be expressed in XX gonads, but only estrogen receptors were expressed in XY gonads at 5 dah.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing, PR China.

ABSTRACT
Four pairs of XX and XY gonads from Nile tilapia were sequenced at four developmental stages, 5, 30, 90, and 180 days after hatching (dah) using Illumina Hiseq(TM) technology. This produced 28 Gb sequences, which were mapped to 21,334 genes. Of these, 259 genes were found to be specifically expressed in XY gonads, and 69 were found to be specific to XX gonads. Totally, 187 XX- and 1,358 XY-enhanced genes were identified, and 2,978 genes were found to be co-expressed in XX and XY gonads. Almost all steroidogenic enzymes, including cyp19a1a, were up-regulated in XX gonads at 5 dah; but in XY gonads these enzymes, including cyp11b2, were significantly up-regulated at 90 dah, indicating that, at a time critical to sex determination, the XX fish produced estrogen and the XY fish did not produce androgens. The most pronounced expression of steroidogenic enzyme genes was observed at 30 and 90 dah for XX and XY gonads, corresponding to the initiation of germ cell meiosis in the female and male gonads, respectively. Both estrogen and androgen receptors were found to be expressed in XX gonads, but only estrogen receptors were expressed in XY gonads at 5 dah. This could explain why exogenous steroid treatment induced XX and XY sex reversal. The XX-enhanced expression of cyp19a1a and cyp19a1b at all stages suggests an important role for estrogen in female sex determination and maintenance of phenotypic sex. This work is the largest collection of gonadal transcriptome data in tilapia and lays the foundation for future studies into the molecular mechanisms of sex determination and maintenance of phenotypic sex in non-model teleosts.

Show MeSH