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Mixed protein-templated luminescent metal clusters (Au and Pt) for H2O2 sensing.

Li M, Yang DP, Wang X, Lu J, Cui D - Nanoscale Res Lett (2013)

Bottom Line: A simple and cost-effective method to synthesize the luminescent noble metal clusters (Au and Pt) in chicken egg white aqueous solution at room temperature is reported.The red-emitting Au cluster is used as fluorescent probe for sensitive detection of H2O2.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical College, Wenzhou, Zhejiang, 325035, China. boyydp@sjtu.edu.cn.

ABSTRACT
A simple and cost-effective method to synthesize the luminescent noble metal clusters (Au and Pt) in chicken egg white aqueous solution at room temperature is reported. The red-emitting Au cluster is used as fluorescent probe for sensitive detection of H2O2.

No MeSH data available.


Spectral Analysis of aqueous solution of chicken egg white and metal clusters. (a) UV-vis absorption spectra; (b) CD spectra.
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Figure 2: Spectral Analysis of aqueous solution of chicken egg white and metal clusters. (a) UV-vis absorption spectra; (b) CD spectra.

Mentions: As well known, metal clusters show obviously different absorption features compared to their corresponding nanoparticles. As shown in Figure 2a, the UV absorption spectra of these sample solutions prepared at various Au3+ concentrations did not indicate any formation of AuNPs due to the absence of localized surface plasmon resonance bands (ca. 520 nm). The absorption peaks at 280 nm could be attributed to the features of aromatic amino acids in proteins. Due to the addition of exogenous agents, the absorption profile of Au and Pt at 280 nm is relatively wider than that of pure egg white, indicating that the variation of the microenvironment has an evident effect to protein conformations. Since circular dichroism (CD) is a kind of effect tool to study proteins’ conformational changes, therefore, we performed CD spectroscopy to reveal their secondary structure changes in detail before and after the formation of metal clusters. As shown in Figure 2b, the CD spectrum of pure egg white aqueous solution displays a negative band around 215 nm and a positive band around 195 nm from the β-sheet as the main structures. However, a negative band around 200 nm from the random coil structure was dominantly observed for the egg white-templated metal clusters. The conformational change indicates that egg white has given rise to denaturation due to the addition of metal ions and strong base.


Mixed protein-templated luminescent metal clusters (Au and Pt) for H2O2 sensing.

Li M, Yang DP, Wang X, Lu J, Cui D - Nanoscale Res Lett (2013)

Spectral Analysis of aqueous solution of chicken egg white and metal clusters. (a) UV-vis absorption spectra; (b) CD spectra.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643886&req=5

Figure 2: Spectral Analysis of aqueous solution of chicken egg white and metal clusters. (a) UV-vis absorption spectra; (b) CD spectra.
Mentions: As well known, metal clusters show obviously different absorption features compared to their corresponding nanoparticles. As shown in Figure 2a, the UV absorption spectra of these sample solutions prepared at various Au3+ concentrations did not indicate any formation of AuNPs due to the absence of localized surface plasmon resonance bands (ca. 520 nm). The absorption peaks at 280 nm could be attributed to the features of aromatic amino acids in proteins. Due to the addition of exogenous agents, the absorption profile of Au and Pt at 280 nm is relatively wider than that of pure egg white, indicating that the variation of the microenvironment has an evident effect to protein conformations. Since circular dichroism (CD) is a kind of effect tool to study proteins’ conformational changes, therefore, we performed CD spectroscopy to reveal their secondary structure changes in detail before and after the formation of metal clusters. As shown in Figure 2b, the CD spectrum of pure egg white aqueous solution displays a negative band around 215 nm and a positive band around 195 nm from the β-sheet as the main structures. However, a negative band around 200 nm from the random coil structure was dominantly observed for the egg white-templated metal clusters. The conformational change indicates that egg white has given rise to denaturation due to the addition of metal ions and strong base.

Bottom Line: A simple and cost-effective method to synthesize the luminescent noble metal clusters (Au and Pt) in chicken egg white aqueous solution at room temperature is reported.The red-emitting Au cluster is used as fluorescent probe for sensitive detection of H2O2.

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory of Medical Genetics, Wenzhou Medical College, Wenzhou, Zhejiang, 325035, China. boyydp@sjtu.edu.cn.

ABSTRACT
A simple and cost-effective method to synthesize the luminescent noble metal clusters (Au and Pt) in chicken egg white aqueous solution at room temperature is reported. The red-emitting Au cluster is used as fluorescent probe for sensitive detection of H2O2.

No MeSH data available.