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A new method of establishing orthotopic bladder transplantable tumor in mice.

Yang XH, Ren LS, Wang GP, Zhao LL, Zhang H, Mi ZG, Bai X - Cancer Biol Med (2012)

Bottom Line: The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity.The drift angle stylet was inserted into the pipe casing slowly, rotated for five times, and then pulled out.A cell suspension (0.1 mL) of approximately 1×10(6) T24 cells (or BTT cells) was then injected immediately.

View Article: PubMed Central - PubMed

Affiliation: Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China.

ABSTRACT

Objective: The present study aims to find a convenient, rapid, and stable method to establish bladder tumor in mice.

Methods: Female Balb/C-nu-nu nude mice (or female T739 mice) were narcotized by sodium pentobarbital at a dosage of 60 mg/kg. The stylet of the 24# venous retention needles was bent in a 5° to 7° angle at a distance of 15 mm from the needlepoint to form a circle with 2.61 mm to 3.66 mm radius when the stylet is rotated. The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity. The drift angle stylet was inserted into the pipe casing slowly, rotated for five times, and then pulled out. A cell suspension (0.1 mL) of approximately 1×10(6) T24 cells (or BTT cells) was then injected immediately.

Results: A total of 60 T739 mice and 60 Balb/C-nu-nu nude mice were inoculated with BTT cells and T24 cells, respectively. The bladder tumor incidence and the average survival time of the tumor-bearing mice were 100% and (26.69±9.24) d and 100% and (34.59±9.8) d for the T739 mice and Balb/C-nu-nu nude mice, respectively.

Conclusions: Using the drift angle stylet to injure the mucous membrane of the urinary bladder can establish a stable bladder transplantable tumor model in mice.

No MeSH data available.


Related in: MedlinePlus

Pathological images of different groups. A: The normal control group bladder (H&E staining, ×100); B: The model group bladder with tumor (H&E staining, ×100); C: The metastasis in liver (H&E staining, ×200); D: The metastasis in kidney (H&E staining, ×200).
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f6: Pathological images of different groups. A: The normal control group bladder (H&E staining, ×100); B: The model group bladder with tumor (H&E staining, ×100); C: The metastasis in liver (H&E staining, ×200); D: The metastasis in kidney (H&E staining, ×200).

Mentions: The bladder mucosa of the normal control mice was intact, and no metastasis was observed (Figure 6A). Huge solid tumors were observed under the microscope. The tumor cells infiltrated deeply into the muscular layer (Figure 6B). Cellular atypia was observed in the pathological section of the liver and kidney (Figures 6C and 6D).


A new method of establishing orthotopic bladder transplantable tumor in mice.

Yang XH, Ren LS, Wang GP, Zhao LL, Zhang H, Mi ZG, Bai X - Cancer Biol Med (2012)

Pathological images of different groups. A: The normal control group bladder (H&E staining, ×100); B: The model group bladder with tumor (H&E staining, ×100); C: The metastasis in liver (H&E staining, ×200); D: The metastasis in kidney (H&E staining, ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643680&req=5

f6: Pathological images of different groups. A: The normal control group bladder (H&E staining, ×100); B: The model group bladder with tumor (H&E staining, ×100); C: The metastasis in liver (H&E staining, ×200); D: The metastasis in kidney (H&E staining, ×200).
Mentions: The bladder mucosa of the normal control mice was intact, and no metastasis was observed (Figure 6A). Huge solid tumors were observed under the microscope. The tumor cells infiltrated deeply into the muscular layer (Figure 6B). Cellular atypia was observed in the pathological section of the liver and kidney (Figures 6C and 6D).

Bottom Line: The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity.The drift angle stylet was inserted into the pipe casing slowly, rotated for five times, and then pulled out.A cell suspension (0.1 mL) of approximately 1×10(6) T24 cells (or BTT cells) was then injected immediately.

View Article: PubMed Central - PubMed

Affiliation: Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China.

ABSTRACT

Objective: The present study aims to find a convenient, rapid, and stable method to establish bladder tumor in mice.

Methods: Female Balb/C-nu-nu nude mice (or female T739 mice) were narcotized by sodium pentobarbital at a dosage of 60 mg/kg. The stylet of the 24# venous retention needles was bent in a 5° to 7° angle at a distance of 15 mm from the needlepoint to form a circle with 2.61 mm to 3.66 mm radius when the stylet is rotated. The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity. The drift angle stylet was inserted into the pipe casing slowly, rotated for five times, and then pulled out. A cell suspension (0.1 mL) of approximately 1×10(6) T24 cells (or BTT cells) was then injected immediately.

Results: A total of 60 T739 mice and 60 Balb/C-nu-nu nude mice were inoculated with BTT cells and T24 cells, respectively. The bladder tumor incidence and the average survival time of the tumor-bearing mice were 100% and (26.69±9.24) d and 100% and (34.59±9.8) d for the T739 mice and Balb/C-nu-nu nude mice, respectively.

Conclusions: Using the drift angle stylet to injure the mucous membrane of the urinary bladder can establish a stable bladder transplantable tumor model in mice.

No MeSH data available.


Related in: MedlinePlus