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A new method of establishing orthotopic bladder transplantable tumor in mice.

Yang XH, Ren LS, Wang GP, Zhao LL, Zhang H, Mi ZG, Bai X - Cancer Biol Med (2012)

Bottom Line: The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity.The drift angle stylet was inserted into the pipe casing slowly, rotated for five times, and then pulled out.A cell suspension (0.1 mL) of approximately 1×10(6) T24 cells (or BTT cells) was then injected immediately.

View Article: PubMed Central - PubMed

Affiliation: Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China.

ABSTRACT

Objective: The present study aims to find a convenient, rapid, and stable method to establish bladder tumor in mice.

Methods: Female Balb/C-nu-nu nude mice (or female T739 mice) were narcotized by sodium pentobarbital at a dosage of 60 mg/kg. The stylet of the 24# venous retention needles was bent in a 5° to 7° angle at a distance of 15 mm from the needlepoint to form a circle with 2.61 mm to 3.66 mm radius when the stylet is rotated. The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity. The drift angle stylet was inserted into the pipe casing slowly, rotated for five times, and then pulled out. A cell suspension (0.1 mL) of approximately 1×10(6) T24 cells (or BTT cells) was then injected immediately.

Results: A total of 60 T739 mice and 60 Balb/C-nu-nu nude mice were inoculated with BTT cells and T24 cells, respectively. The bladder tumor incidence and the average survival time of the tumor-bearing mice were 100% and (26.69±9.24) d and 100% and (34.59±9.8) d for the T739 mice and Balb/C-nu-nu nude mice, respectively.

Conclusions: Using the drift angle stylet to injure the mucous membrane of the urinary bladder can establish a stable bladder transplantable tumor model in mice.

No MeSH data available.


Related in: MedlinePlus

Comparison between the mucosa injured and mucosa uninjured urinary bladder. A: mucosa uninjured; B: mucosa injured. The bladders were injected with physiological saline.
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f2: Comparison between the mucosa injured and mucosa uninjured urinary bladder. A: mucosa uninjured; B: mucosa injured. The bladders were injected with physiological saline.

Mentions: The mice in the experimental group were narcotized by sodium pentobarbital at a dosage of 60 mg/kg. The pipe casing was lubricated with liquid paraffin and inserted into the bladder cavity, and then urine was removed. The drift angle stylet was slowly inserted into the pipe casing. The pipe casing was fixed with one hand, the stylet was rotated for five rounds with the other hand, and then pulled out. Cell suspension (100 µL) of approximately 1×106 cells was injected immediately. The mice in the normal control group were injected with 1×106 cells directly without mucosa injury. The comparison results between the mucosa injured and uninjured urinary bladder are shown in Figure 2.


A new method of establishing orthotopic bladder transplantable tumor in mice.

Yang XH, Ren LS, Wang GP, Zhao LL, Zhang H, Mi ZG, Bai X - Cancer Biol Med (2012)

Comparison between the mucosa injured and mucosa uninjured urinary bladder. A: mucosa uninjured; B: mucosa injured. The bladders were injected with physiological saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643680&req=5

f2: Comparison between the mucosa injured and mucosa uninjured urinary bladder. A: mucosa uninjured; B: mucosa injured. The bladders were injected with physiological saline.
Mentions: The mice in the experimental group were narcotized by sodium pentobarbital at a dosage of 60 mg/kg. The pipe casing was lubricated with liquid paraffin and inserted into the bladder cavity, and then urine was removed. The drift angle stylet was slowly inserted into the pipe casing. The pipe casing was fixed with one hand, the stylet was rotated for five rounds with the other hand, and then pulled out. Cell suspension (100 µL) of approximately 1×106 cells was injected immediately. The mice in the normal control group were injected with 1×106 cells directly without mucosa injury. The comparison results between the mucosa injured and uninjured urinary bladder are shown in Figure 2.

Bottom Line: The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity.The drift angle stylet was inserted into the pipe casing slowly, rotated for five times, and then pulled out.A cell suspension (0.1 mL) of approximately 1×10(6) T24 cells (or BTT cells) was then injected immediately.

View Article: PubMed Central - PubMed

Affiliation: Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China.

ABSTRACT

Objective: The present study aims to find a convenient, rapid, and stable method to establish bladder tumor in mice.

Methods: Female Balb/C-nu-nu nude mice (or female T739 mice) were narcotized by sodium pentobarbital at a dosage of 60 mg/kg. The stylet of the 24# venous retention needles was bent in a 5° to 7° angle at a distance of 15 mm from the needlepoint to form a circle with 2.61 mm to 3.66 mm radius when the stylet is rotated. The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity. The drift angle stylet was inserted into the pipe casing slowly, rotated for five times, and then pulled out. A cell suspension (0.1 mL) of approximately 1×10(6) T24 cells (or BTT cells) was then injected immediately.

Results: A total of 60 T739 mice and 60 Balb/C-nu-nu nude mice were inoculated with BTT cells and T24 cells, respectively. The bladder tumor incidence and the average survival time of the tumor-bearing mice were 100% and (26.69±9.24) d and 100% and (34.59±9.8) d for the T739 mice and Balb/C-nu-nu nude mice, respectively.

Conclusions: Using the drift angle stylet to injure the mucous membrane of the urinary bladder can establish a stable bladder transplantable tumor model in mice.

No MeSH data available.


Related in: MedlinePlus