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Functionalized gold nanorods for tumor imaging and targeted therapy.

Gui C, Cui DX - Cancer Biol Med (2012)

Bottom Line: Gold nanorods, as an emerging noble metal nanomaterial with unique properties, have become the new exciting focus of theoretical and experimental studies in the past few years.The structure and function of gold nanorods, especially their biocompatibility, optical property, and photothermal effects, have been attracting more and more attention.We also explore other prospective applications and discuss the corresponding concepts, issues, approaches, and challenges, with the aim of stimulating broader interest in gold nanorod-based nanotechnology and improving its practical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiaotong University, Shanghai 200240, China.

ABSTRACT
Gold nanorods, as an emerging noble metal nanomaterial with unique properties, have become the new exciting focus of theoretical and experimental studies in the past few years. The structure and function of gold nanorods, especially their biocompatibility, optical property, and photothermal effects, have been attracting more and more attention. Gold nanorods exhibit great potential in applications such as tumor molecular imaging and photothermal therapy. In this article, we review some of the main advances made over the past few years in the application of gold nanorods in surface functionalization, molecular imaging, and photothermal therapy. We also explore other prospective applications and discuss the corresponding concepts, issues, approaches, and challenges, with the aim of stimulating broader interest in gold nanorod-based nanotechnology and improving its practical application.

No MeSH data available.


Related in: MedlinePlus

Dark-field scattering images. A: Low-magnification image; B-D: High-magnification images of the MGC803 cells incubated with 50 µmol/L of GNFs for 2 h and monitored by dark-field microscopy; E, Effect of photothermal therapy on MGC803 cells at the boundary of the laser spot incubated with 10 µmol/L GNFs for 24 h at 37°C in the dark prior to irradiation for 5 min with a 632.8 nm He–Ne laser with energy density of ~30 mW/cm2; F: MGC803 cells on the laser spot. Reprinted with permission from [40], Huang P, Bao L, Zhang CL, et al. Folic acid-conjugated Silica-modified gold nanorods for X-ray/CT imaging-guided dual-mode radiation and photo-thermal therapy. Biomaterials 2011; 32: 9796-9809.
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f16: Dark-field scattering images. A: Low-magnification image; B-D: High-magnification images of the MGC803 cells incubated with 50 µmol/L of GNFs for 2 h and monitored by dark-field microscopy; E, Effect of photothermal therapy on MGC803 cells at the boundary of the laser spot incubated with 10 µmol/L GNFs for 24 h at 37°C in the dark prior to irradiation for 5 min with a 632.8 nm He–Ne laser with energy density of ~30 mW/cm2; F: MGC803 cells on the laser spot. Reprinted with permission from [40], Huang P, Bao L, Zhang CL, et al. Folic acid-conjugated Silica-modified gold nanorods for X-ray/CT imaging-guided dual-mode radiation and photo-thermal therapy. Biomaterials 2011; 32: 9796-9809.

Mentions: To analyze further the biomedical application of chiral GNFs, Huang et al.[41] also measured the dark cytotoxity of the cells. The results showed that chiral GNFs were non-cytotoxic and had excellent biocompatibility with MGC803 cells within the concentration range of 0 to 200 µmol/L. To determine the intracellular uptake of chiral GNFs, dark-field scattering imaging was performed on the MGC803 cells (Figure 16). As shown in Figure 16 (C and D), the cells displayed an intense homogeneous cytoplasmic golden color around the nucleus. This observation indicated that GNFs accumulated in the cells. Through irradiation by a 632.8 nm He-Ne laser, the GNFs could convert the absorbed photons into thermal energy for tumor cell PTT. On the boundary of the laser spots, the cells without irradiation were in good physiological state and appeared spindle-like (Figure 16E). On the laser spot, all the cells were killed. The dead cells exhibited blue nuclei (Figure 16F). The above results suggested that the as-synthesized GNFs with good biocompatibility can be utilized in cellular dark-field imaging and photothermal therapy.


Functionalized gold nanorods for tumor imaging and targeted therapy.

Gui C, Cui DX - Cancer Biol Med (2012)

Dark-field scattering images. A: Low-magnification image; B-D: High-magnification images of the MGC803 cells incubated with 50 µmol/L of GNFs for 2 h and monitored by dark-field microscopy; E, Effect of photothermal therapy on MGC803 cells at the boundary of the laser spot incubated with 10 µmol/L GNFs for 24 h at 37°C in the dark prior to irradiation for 5 min with a 632.8 nm He–Ne laser with energy density of ~30 mW/cm2; F: MGC803 cells on the laser spot. Reprinted with permission from [40], Huang P, Bao L, Zhang CL, et al. Folic acid-conjugated Silica-modified gold nanorods for X-ray/CT imaging-guided dual-mode radiation and photo-thermal therapy. Biomaterials 2011; 32: 9796-9809.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643678&req=5

f16: Dark-field scattering images. A: Low-magnification image; B-D: High-magnification images of the MGC803 cells incubated with 50 µmol/L of GNFs for 2 h and monitored by dark-field microscopy; E, Effect of photothermal therapy on MGC803 cells at the boundary of the laser spot incubated with 10 µmol/L GNFs for 24 h at 37°C in the dark prior to irradiation for 5 min with a 632.8 nm He–Ne laser with energy density of ~30 mW/cm2; F: MGC803 cells on the laser spot. Reprinted with permission from [40], Huang P, Bao L, Zhang CL, et al. Folic acid-conjugated Silica-modified gold nanorods for X-ray/CT imaging-guided dual-mode radiation and photo-thermal therapy. Biomaterials 2011; 32: 9796-9809.
Mentions: To analyze further the biomedical application of chiral GNFs, Huang et al.[41] also measured the dark cytotoxity of the cells. The results showed that chiral GNFs were non-cytotoxic and had excellent biocompatibility with MGC803 cells within the concentration range of 0 to 200 µmol/L. To determine the intracellular uptake of chiral GNFs, dark-field scattering imaging was performed on the MGC803 cells (Figure 16). As shown in Figure 16 (C and D), the cells displayed an intense homogeneous cytoplasmic golden color around the nucleus. This observation indicated that GNFs accumulated in the cells. Through irradiation by a 632.8 nm He-Ne laser, the GNFs could convert the absorbed photons into thermal energy for tumor cell PTT. On the boundary of the laser spots, the cells without irradiation were in good physiological state and appeared spindle-like (Figure 16E). On the laser spot, all the cells were killed. The dead cells exhibited blue nuclei (Figure 16F). The above results suggested that the as-synthesized GNFs with good biocompatibility can be utilized in cellular dark-field imaging and photothermal therapy.

Bottom Line: Gold nanorods, as an emerging noble metal nanomaterial with unique properties, have become the new exciting focus of theoretical and experimental studies in the past few years.The structure and function of gold nanorods, especially their biocompatibility, optical property, and photothermal effects, have been attracting more and more attention.We also explore other prospective applications and discuss the corresponding concepts, issues, approaches, and challenges, with the aim of stimulating broader interest in gold nanorod-based nanotechnology and improving its practical application.

View Article: PubMed Central - PubMed

Affiliation: Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Institute of Micro-Nano Science and Technology, Shanghai Jiaotong University, Shanghai 200240, China.

ABSTRACT
Gold nanorods, as an emerging noble metal nanomaterial with unique properties, have become the new exciting focus of theoretical and experimental studies in the past few years. The structure and function of gold nanorods, especially their biocompatibility, optical property, and photothermal effects, have been attracting more and more attention. Gold nanorods exhibit great potential in applications such as tumor molecular imaging and photothermal therapy. In this article, we review some of the main advances made over the past few years in the application of gold nanorods in surface functionalization, molecular imaging, and photothermal therapy. We also explore other prospective applications and discuss the corresponding concepts, issues, approaches, and challenges, with the aim of stimulating broader interest in gold nanorod-based nanotechnology and improving its practical application.

No MeSH data available.


Related in: MedlinePlus