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Expression of Angiotensin II and Aldosterone in Radiation-induced Lung Injury.

Cao S, Wu R - Cancer Biol Med (2012)

Bottom Line: Considering its negative impact on patients and restrictions to efficacy, the mechanism of RILI was studied.Moreover, the expression level of the angiotensin II-aldosterone system increased with increasing doses, and the difference was still observed as time progressed.Angiotensin II-aldosterone system has an important pathophysiological function in the progression of RILI.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Shengjing Hospital of China Medical University, Shenyang 110022, China.

ABSTRACT

Objective: Radiation-induced lung injury (RILI) is the most common, dose-limiting complication in thoracic malignancy radiotherapy. Considering its negative impact on patients and restrictions to efficacy, the mechanism of RILI was studied.

Methods: Wistar rats were locally irradiated with a single dose of 0, 16, and 20 Gy to the right half of the lung to establish a lung injury model. Two and six months after irradiation, the right half of the rat lung tissue was removed, and the concentrations of TGF-β1, angiotensin II, and aldosterone were determined via enzyme-linked immunosorbent assay.

Results: Statistical differences were observed in the expression levels of angiotensin II and aldosterone between the non-irradiation and irradiation groups. Moreover, the expression level of the angiotensin II-aldosterone system increased with increasing doses, and the difference was still observed as time progressed.

Conclusions: Angiotensin II-aldosterone system has an important pathophysiological function in the progression of RILI.

No MeSH data available.


Related in: MedlinePlus

Concentration of aldosterone at two months.
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f5: Concentration of aldosterone at two months.

Mentions: As demonstrated in Table 1 and Figure 1, the mean TGF-β1 level (at two months) was (406.43±120.59) pg/mL for the control group, and (550.17±90.51) pg/mL and (774.20±160.77) pg/mL for the treated groups (16 Gy and 20 Gy, respectively). The rats that developed RILI showed a statistically significant higher level of TGF-β1 (P=0.005, P<0.001). The TGF-β1 level increased with increasing radiation dose, and the value for the 20 Gy group was higher than that for the 16 Gy group (P<0.001). However, Table 2 and Figure 2 show the TGF-β1 level at six months, where the mean level was (498.55±49.55)pg/mL, (802.23±136.71) pg/mL, (1004.59±86.89) pg/mL for the control and treated groups (16 Gy and 20 Gy), respectively. The concentrations for the treated groups were higher (P=0.003, P<0.001), and differences were detected between the two irradiation groups (P=0.037). The mean values of the expression of angiotensin II for two months are shown in Table 3 and Figure 3. Significant differences in the expression level of angiotensin II were observed between non-irradiation and irradiation groups (P=0.005, P<0.001). Moreover, statistical differences were observed between the two irradiation groups (P<0.001). As shown in Table 4 and Figure 4, the angiotensin II concentration in the irradiation groups was higher than that in the control group (P=0.006, P<0.001), and differences were also detected between the two irradiation groups (P=0.004). The mean values of the aldosterone expression at two months are shown in Table 5 and Figure 5. The aldosterone concentration in the irradiation groups was higher than that in the control group (P=0.01, P<0.001), but significant differences were not detected between the two irradiation groups (P=0.131). Table 6 and Figure 6 show the aldosterone level at six months. The concentration in the treated groups was higher than that in the control group (P=0.006, P<0.001), and differences were detected between the two irradiation groups (P=0.03). Lung tissue inflammatory lesions and fibrosis were observed by H&E stains in the irradiation groups (Figures 7,8,9,10,11,12).


Expression of Angiotensin II and Aldosterone in Radiation-induced Lung Injury.

Cao S, Wu R - Cancer Biol Med (2012)

Concentration of aldosterone at two months.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643675&req=5

f5: Concentration of aldosterone at two months.
Mentions: As demonstrated in Table 1 and Figure 1, the mean TGF-β1 level (at two months) was (406.43±120.59) pg/mL for the control group, and (550.17±90.51) pg/mL and (774.20±160.77) pg/mL for the treated groups (16 Gy and 20 Gy, respectively). The rats that developed RILI showed a statistically significant higher level of TGF-β1 (P=0.005, P<0.001). The TGF-β1 level increased with increasing radiation dose, and the value for the 20 Gy group was higher than that for the 16 Gy group (P<0.001). However, Table 2 and Figure 2 show the TGF-β1 level at six months, where the mean level was (498.55±49.55)pg/mL, (802.23±136.71) pg/mL, (1004.59±86.89) pg/mL for the control and treated groups (16 Gy and 20 Gy), respectively. The concentrations for the treated groups were higher (P=0.003, P<0.001), and differences were detected between the two irradiation groups (P=0.037). The mean values of the expression of angiotensin II for two months are shown in Table 3 and Figure 3. Significant differences in the expression level of angiotensin II were observed between non-irradiation and irradiation groups (P=0.005, P<0.001). Moreover, statistical differences were observed between the two irradiation groups (P<0.001). As shown in Table 4 and Figure 4, the angiotensin II concentration in the irradiation groups was higher than that in the control group (P=0.006, P<0.001), and differences were also detected between the two irradiation groups (P=0.004). The mean values of the aldosterone expression at two months are shown in Table 5 and Figure 5. The aldosterone concentration in the irradiation groups was higher than that in the control group (P=0.01, P<0.001), but significant differences were not detected between the two irradiation groups (P=0.131). Table 6 and Figure 6 show the aldosterone level at six months. The concentration in the treated groups was higher than that in the control group (P=0.006, P<0.001), and differences were detected between the two irradiation groups (P=0.03). Lung tissue inflammatory lesions and fibrosis were observed by H&E stains in the irradiation groups (Figures 7,8,9,10,11,12).

Bottom Line: Considering its negative impact on patients and restrictions to efficacy, the mechanism of RILI was studied.Moreover, the expression level of the angiotensin II-aldosterone system increased with increasing doses, and the difference was still observed as time progressed.Angiotensin II-aldosterone system has an important pathophysiological function in the progression of RILI.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Shengjing Hospital of China Medical University, Shenyang 110022, China.

ABSTRACT

Objective: Radiation-induced lung injury (RILI) is the most common, dose-limiting complication in thoracic malignancy radiotherapy. Considering its negative impact on patients and restrictions to efficacy, the mechanism of RILI was studied.

Methods: Wistar rats were locally irradiated with a single dose of 0, 16, and 20 Gy to the right half of the lung to establish a lung injury model. Two and six months after irradiation, the right half of the rat lung tissue was removed, and the concentrations of TGF-β1, angiotensin II, and aldosterone were determined via enzyme-linked immunosorbent assay.

Results: Statistical differences were observed in the expression levels of angiotensin II and aldosterone between the non-irradiation and irradiation groups. Moreover, the expression level of the angiotensin II-aldosterone system increased with increasing doses, and the difference was still observed as time progressed.

Conclusions: Angiotensin II-aldosterone system has an important pathophysiological function in the progression of RILI.

No MeSH data available.


Related in: MedlinePlus