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Quantum dots for cancer research: current status, remaining issues, and future perspectives.

Fang M, Peng CW, Pang DW, Li Y - Cancer Biol Med (2012)

Bottom Line: Cancer is a major threat to public health in the 21st century because it is one of the leading causes of death worldwide.The mechanisms of carcinogenesis, cancer invasion, and metastasis remain unclear.Thus, the development of a novel approach for cancer detection is urgent, and real-time monitoring is crucial in revealing its underlying biological mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, China.

ABSTRACT
Cancer is a major threat to public health in the 21st century because it is one of the leading causes of death worldwide. The mechanisms of carcinogenesis, cancer invasion, and metastasis remain unclear. Thus, the development of a novel approach for cancer detection is urgent, and real-time monitoring is crucial in revealing its underlying biological mechanisms. With the optical and chemical advantages of quantum dots (QDs), QD-based nanotechnology is helpful in constructing a biomedical imaging platform for cancer behavior study. This review mainly focuses on the application of QD-based nanotechnology in cancer cell imaging and tumor microenvironment studies both in vivo and in vitro, as well as the remaining issues and future perspectives.

No MeSH data available.


Related in: MedlinePlus

The establishment of multiplexed QD imaging and spectrum analysis. A1, B1, and C1: Infiltrating macrophages (Green arrows), type IV collagen (Yellow arrows), and neovessels (Red arrows) are labeled simultaneously in gastric cancer tissues with nanoprobes QDs-525, QDs-585, and QDs-655, respectively. A2, B2, and C2: Corresponding unmixed image of A1, B1, and C1 obtained by spectrum analysis with differentiable autofluorescence (Blue arrows). D: QD emission spectra and tissue autofluorescence data used for unmixed image. Magnification: ×100 (A1 and A2), ×200 (B1 and B2), and ×400 (C1 and C2); Scale bar: 100 mm (A1 and A2), 50 mm (B1 and B2), and 20 mm (C1 and C2). Reproduced with permission from [103].
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f3: The establishment of multiplexed QD imaging and spectrum analysis. A1, B1, and C1: Infiltrating macrophages (Green arrows), type IV collagen (Yellow arrows), and neovessels (Red arrows) are labeled simultaneously in gastric cancer tissues with nanoprobes QDs-525, QDs-585, and QDs-655, respectively. A2, B2, and C2: Corresponding unmixed image of A1, B1, and C1 obtained by spectrum analysis with differentiable autofluorescence (Blue arrows). D: QD emission spectra and tissue autofluorescence data used for unmixed image. Magnification: ×100 (A1 and A2), ×200 (B1 and B2), and ×400 (C1 and C2); Scale bar: 100 mm (A1 and A2), 50 mm (B1 and B2), and 20 mm (C1 and C2). Reproduced with permission from [103].

Mentions: Our group recently confirmed the benefit of QD-based multiplexed imaging and spectrum analysis technology to study the co-evolution of cancer cells and tumor stroma by type IV collagen, tumor angiogenesis, macrophage infiltration, and tissue destructive proteolytic enzyme MMP9[107,108], which revealed the related molecular features of tumor microenvironment during cancer invasion[108] (Figure 3). Four invasive patterns with distinctive cancer cell-stroma interactions were identified, namely, washing, amoeba-like, polar, and linear patterns. Another research on QDbased doublecolor imaging of HER2 on BC cells and the type IV collagen in the ECM also showcase the dynamic processes of BC invasion (Figure 4)[69].


Quantum dots for cancer research: current status, remaining issues, and future perspectives.

Fang M, Peng CW, Pang DW, Li Y - Cancer Biol Med (2012)

The establishment of multiplexed QD imaging and spectrum analysis. A1, B1, and C1: Infiltrating macrophages (Green arrows), type IV collagen (Yellow arrows), and neovessels (Red arrows) are labeled simultaneously in gastric cancer tissues with nanoprobes QDs-525, QDs-585, and QDs-655, respectively. A2, B2, and C2: Corresponding unmixed image of A1, B1, and C1 obtained by spectrum analysis with differentiable autofluorescence (Blue arrows). D: QD emission spectra and tissue autofluorescence data used for unmixed image. Magnification: ×100 (A1 and A2), ×200 (B1 and B2), and ×400 (C1 and C2); Scale bar: 100 mm (A1 and A2), 50 mm (B1 and B2), and 20 mm (C1 and C2). Reproduced with permission from [103].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643664&req=5

f3: The establishment of multiplexed QD imaging and spectrum analysis. A1, B1, and C1: Infiltrating macrophages (Green arrows), type IV collagen (Yellow arrows), and neovessels (Red arrows) are labeled simultaneously in gastric cancer tissues with nanoprobes QDs-525, QDs-585, and QDs-655, respectively. A2, B2, and C2: Corresponding unmixed image of A1, B1, and C1 obtained by spectrum analysis with differentiable autofluorescence (Blue arrows). D: QD emission spectra and tissue autofluorescence data used for unmixed image. Magnification: ×100 (A1 and A2), ×200 (B1 and B2), and ×400 (C1 and C2); Scale bar: 100 mm (A1 and A2), 50 mm (B1 and B2), and 20 mm (C1 and C2). Reproduced with permission from [103].
Mentions: Our group recently confirmed the benefit of QD-based multiplexed imaging and spectrum analysis technology to study the co-evolution of cancer cells and tumor stroma by type IV collagen, tumor angiogenesis, macrophage infiltration, and tissue destructive proteolytic enzyme MMP9[107,108], which revealed the related molecular features of tumor microenvironment during cancer invasion[108] (Figure 3). Four invasive patterns with distinctive cancer cell-stroma interactions were identified, namely, washing, amoeba-like, polar, and linear patterns. Another research on QDbased doublecolor imaging of HER2 on BC cells and the type IV collagen in the ECM also showcase the dynamic processes of BC invasion (Figure 4)[69].

Bottom Line: Cancer is a major threat to public health in the 21st century because it is one of the leading causes of death worldwide.The mechanisms of carcinogenesis, cancer invasion, and metastasis remain unclear.Thus, the development of a novel approach for cancer detection is urgent, and real-time monitoring is crucial in revealing its underlying biological mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors & Hubei Cancer Clinical Study Center, Wuhan 430071, China.

ABSTRACT
Cancer is a major threat to public health in the 21st century because it is one of the leading causes of death worldwide. The mechanisms of carcinogenesis, cancer invasion, and metastasis remain unclear. Thus, the development of a novel approach for cancer detection is urgent, and real-time monitoring is crucial in revealing its underlying biological mechanisms. With the optical and chemical advantages of quantum dots (QDs), QD-based nanotechnology is helpful in constructing a biomedical imaging platform for cancer behavior study. This review mainly focuses on the application of QD-based nanotechnology in cancer cell imaging and tumor microenvironment studies both in vivo and in vitro, as well as the remaining issues and future perspectives.

No MeSH data available.


Related in: MedlinePlus