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Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus.

Zheng HY, Zhang XY, Wang XF, Sun BC - Cancer Biol Med (2012)

Bottom Line: LC3B was expressed both in cancer cells and normal epithelial cells.LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002).Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

ABSTRACT

Objective: To investigate LC3B-II and active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy, and to explore the relationship of autophagy with apoptosis in human colorectal cancer.

Methods: LC3B expression was detected by immunohistochemistry in 53 human colorectal cancer tissues and 20 normal colon tissues. The protein levels of LC3B-II and active caspase-3 were also determined by Western blot analysis in 23 human colorectal cancer tissues and 10 normal colon tissues.

Results: LC3B was expressed both in cancer cells and normal epithelial cells. LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002). The protein level of LC3B-II in cancer tissue was significantly higher than in normal tissue (P=0.038), but the expression of active forms of procaspase-3 in cancer tissue was lower (P=0.041). There was a statistically significant positive correlation between the expression levels of LC3B-II and the active forms of procaspase-3 (r=0.537, P=0.008).

Conclusions: Autophagy has a prosurvival role in human colorectal cancer. Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

No MeSH data available.


Related in: MedlinePlus

Expression of LC3B-II, procaspase-3, and active forms of caspase-3 in normal tissues (1N, 2N) and cancer tissues (1C, 2C) detected by Western blot analysis.
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f4: Expression of LC3B-II, procaspase-3, and active forms of caspase-3 in normal tissues (1N, 2N) and cancer tissues (1C, 2C) detected by Western blot analysis.

Mentions: The expression of LC3B-II and active caspase-3 in 23 human colorectal cancer tissues and 10 normal colorectal tissues was detected by Western blot analysis. As shown in Figure 4, LC3B-II, procaspase-3, and its corresponding active form caspase-3 were observed in normal colon and cancer tissues. The Western blot showed that the protein levels of LC3B-II in cancer tissue were significantly higher than those in normal tissue (P=0.038). The active forms of procaspase-3 were markedly reduced in cancer tissue than in normal tissue (P=0.041). Analysis of the correlation of LC3B-II with the active forms of caspase-3 indicated a statistically significant positive correlation between the expression of LC3B-II and the active forms of caspase-3 (r=0.537, P=0.008).


Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus.

Zheng HY, Zhang XY, Wang XF, Sun BC - Cancer Biol Med (2012)

Expression of LC3B-II, procaspase-3, and active forms of caspase-3 in normal tissues (1N, 2N) and cancer tissues (1C, 2C) detected by Western blot analysis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643655&req=5

f4: Expression of LC3B-II, procaspase-3, and active forms of caspase-3 in normal tissues (1N, 2N) and cancer tissues (1C, 2C) detected by Western blot analysis.
Mentions: The expression of LC3B-II and active caspase-3 in 23 human colorectal cancer tissues and 10 normal colorectal tissues was detected by Western blot analysis. As shown in Figure 4, LC3B-II, procaspase-3, and its corresponding active form caspase-3 were observed in normal colon and cancer tissues. The Western blot showed that the protein levels of LC3B-II in cancer tissue were significantly higher than those in normal tissue (P=0.038). The active forms of procaspase-3 were markedly reduced in cancer tissue than in normal tissue (P=0.041). Analysis of the correlation of LC3B-II with the active forms of caspase-3 indicated a statistically significant positive correlation between the expression of LC3B-II and the active forms of caspase-3 (r=0.537, P=0.008).

Bottom Line: LC3B was expressed both in cancer cells and normal epithelial cells.LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002).Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

ABSTRACT

Objective: To investigate LC3B-II and active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy, and to explore the relationship of autophagy with apoptosis in human colorectal cancer.

Methods: LC3B expression was detected by immunohistochemistry in 53 human colorectal cancer tissues and 20 normal colon tissues. The protein levels of LC3B-II and active caspase-3 were also determined by Western blot analysis in 23 human colorectal cancer tissues and 10 normal colon tissues.

Results: LC3B was expressed both in cancer cells and normal epithelial cells. LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002). The protein level of LC3B-II in cancer tissue was significantly higher than in normal tissue (P=0.038), but the expression of active forms of procaspase-3 in cancer tissue was lower (P=0.041). There was a statistically significant positive correlation between the expression levels of LC3B-II and the active forms of procaspase-3 (r=0.537, P=0.008).

Conclusions: Autophagy has a prosurvival role in human colorectal cancer. Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

No MeSH data available.


Related in: MedlinePlus