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Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus.

Zheng HY, Zhang XY, Wang XF, Sun BC - Cancer Biol Med (2012)

Bottom Line: LC3B was expressed both in cancer cells and normal epithelial cells.LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002).Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

ABSTRACT

Objective: To investigate LC3B-II and active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy, and to explore the relationship of autophagy with apoptosis in human colorectal cancer.

Methods: LC3B expression was detected by immunohistochemistry in 53 human colorectal cancer tissues and 20 normal colon tissues. The protein levels of LC3B-II and active caspase-3 were also determined by Western blot analysis in 23 human colorectal cancer tissues and 10 normal colon tissues.

Results: LC3B was expressed both in cancer cells and normal epithelial cells. LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002). The protein level of LC3B-II in cancer tissue was significantly higher than in normal tissue (P=0.038), but the expression of active forms of procaspase-3 in cancer tissue was lower (P=0.041). There was a statistically significant positive correlation between the expression levels of LC3B-II and the active forms of procaspase-3 (r=0.537, P=0.008).

Conclusions: Autophagy has a prosurvival role in human colorectal cancer. Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

No MeSH data available.


Related in: MedlinePlus

LC3B protein was detected in human colorectal cancer tissues and normal tissues by immunohistochemical staining with LC3B antibody. A: Columnar epithelial cells of normal colon mucosa expressing LC3B; original magnification, × 40. B, D: LC3B expression in poorly differentiated cancer; original magnification, × 20 and × 40. C: LC3B expression in moderately differentiated cancer; original magnification, × 20.
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f2: LC3B protein was detected in human colorectal cancer tissues and normal tissues by immunohistochemical staining with LC3B antibody. A: Columnar epithelial cells of normal colon mucosa expressing LC3B; original magnification, × 40. B, D: LC3B expression in poorly differentiated cancer; original magnification, × 20 and × 40. C: LC3B expression in moderately differentiated cancer; original magnification, × 20.

Mentions: Almost all 53 primary colorectal cancer specimens expressed LC3B as revealed by immunohistochemistry with anti-LC3B antibody (Figure 2). However, LC3B expression in a tumor specimen was usually heterogeneous, with the expression in the peripheral area of the cancer tissue (Figure 3) stronger than in the central area (Figure 3) in most cases. Thus, the cancer tissues were divided into central and peripheral areas. An analysis was performed to explore the relationships of the levels of LC3B expression (strongly positive or weakly positive) in the central or peripheral area of the cancer tissues with the clinicopathologic factors, including age, gender, tumor site, tumor size, differentiation, tumor margin, pT, pN, pStage, tumor necrosis, as well as vessel and nerve plexus invasion. The intensity level of LC3B expression in the peripheral area of the tumor was significantly correlated with the differentiation (P=0.002), tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002) (Table 1). However, the intensity level of LC3B expression in the central area was not correlated with these clinicopathologic factors (data not shown).


Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus.

Zheng HY, Zhang XY, Wang XF, Sun BC - Cancer Biol Med (2012)

LC3B protein was detected in human colorectal cancer tissues and normal tissues by immunohistochemical staining with LC3B antibody. A: Columnar epithelial cells of normal colon mucosa expressing LC3B; original magnification, × 40. B, D: LC3B expression in poorly differentiated cancer; original magnification, × 20 and × 40. C: LC3B expression in moderately differentiated cancer; original magnification, × 20.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643655&req=5

f2: LC3B protein was detected in human colorectal cancer tissues and normal tissues by immunohistochemical staining with LC3B antibody. A: Columnar epithelial cells of normal colon mucosa expressing LC3B; original magnification, × 40. B, D: LC3B expression in poorly differentiated cancer; original magnification, × 20 and × 40. C: LC3B expression in moderately differentiated cancer; original magnification, × 20.
Mentions: Almost all 53 primary colorectal cancer specimens expressed LC3B as revealed by immunohistochemistry with anti-LC3B antibody (Figure 2). However, LC3B expression in a tumor specimen was usually heterogeneous, with the expression in the peripheral area of the cancer tissue (Figure 3) stronger than in the central area (Figure 3) in most cases. Thus, the cancer tissues were divided into central and peripheral areas. An analysis was performed to explore the relationships of the levels of LC3B expression (strongly positive or weakly positive) in the central or peripheral area of the cancer tissues with the clinicopathologic factors, including age, gender, tumor site, tumor size, differentiation, tumor margin, pT, pN, pStage, tumor necrosis, as well as vessel and nerve plexus invasion. The intensity level of LC3B expression in the peripheral area of the tumor was significantly correlated with the differentiation (P=0.002), tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002) (Table 1). However, the intensity level of LC3B expression in the central area was not correlated with these clinicopathologic factors (data not shown).

Bottom Line: LC3B was expressed both in cancer cells and normal epithelial cells.LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002).Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

ABSTRACT

Objective: To investigate LC3B-II and active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy, and to explore the relationship of autophagy with apoptosis in human colorectal cancer.

Methods: LC3B expression was detected by immunohistochemistry in 53 human colorectal cancer tissues and 20 normal colon tissues. The protein levels of LC3B-II and active caspase-3 were also determined by Western blot analysis in 23 human colorectal cancer tissues and 10 normal colon tissues.

Results: LC3B was expressed both in cancer cells and normal epithelial cells. LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002). The protein level of LC3B-II in cancer tissue was significantly higher than in normal tissue (P=0.038), but the expression of active forms of procaspase-3 in cancer tissue was lower (P=0.041). There was a statistically significant positive correlation between the expression levels of LC3B-II and the active forms of procaspase-3 (r=0.537, P=0.008).

Conclusions: Autophagy has a prosurvival role in human colorectal cancer. Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

No MeSH data available.


Related in: MedlinePlus