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Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus.

Zheng HY, Zhang XY, Wang XF, Sun BC - Cancer Biol Med (2012)

Bottom Line: LC3B was expressed both in cancer cells and normal epithelial cells.LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002).Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

ABSTRACT

Objective: To investigate LC3B-II and active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy, and to explore the relationship of autophagy with apoptosis in human colorectal cancer.

Methods: LC3B expression was detected by immunohistochemistry in 53 human colorectal cancer tissues and 20 normal colon tissues. The protein levels of LC3B-II and active caspase-3 were also determined by Western blot analysis in 23 human colorectal cancer tissues and 10 normal colon tissues.

Results: LC3B was expressed both in cancer cells and normal epithelial cells. LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002). The protein level of LC3B-II in cancer tissue was significantly higher than in normal tissue (P=0.038), but the expression of active forms of procaspase-3 in cancer tissue was lower (P=0.041). There was a statistically significant positive correlation between the expression levels of LC3B-II and the active forms of procaspase-3 (r=0.537, P=0.008).

Conclusions: Autophagy has a prosurvival role in human colorectal cancer. Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

No MeSH data available.


Related in: MedlinePlus

LC3B protein was detected in human colorectal cancer tissues by immunohistochemical staining with LC3B antibody. A: LC3B expression in cancer cells showing equal or stronger intensity than in nerve cells (arrow) were deemed “strongly positive.” B: LC3B expression in cancer cells showing weaker intensity than in nerve cells (arrow) were deemed “weakly positive”. Original magnification, × 40.
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f1: LC3B protein was detected in human colorectal cancer tissues by immunohistochemical staining with LC3B antibody. A: LC3B expression in cancer cells showing equal or stronger intensity than in nerve cells (arrow) were deemed “strongly positive.” B: LC3B expression in cancer cells showing weaker intensity than in nerve cells (arrow) were deemed “weakly positive”. Original magnification, × 40.

Mentions: The nerve plexus cells in the submucosal or muscularis portions of the colorectal wall showed constantly intensive staining for LC3B [19]. Thus, each section had its own positive internal control. On this basis, LC3B expression in tumor cells or in normal epithelia was compared with that in nerve cells of the same sections. The following subgroups of nerve cells were then identified: i) those with similar or enhanced LC3B expression in tumor cells or normal epithelia compared with those in nerve cells were judged as ‘‘strongly positive” (Figure 1A), and ii) those with weaker LC3B expression in tumor cells or normal epithelia than those in nerve cells were designated as ‘‘weakly positive’’ (Figure 1B). All immunostaining experiments were evaluated twice by 2 pathologists blinded to the patient outcome and other clinical findings.


Autophagy enhances the aggressiveness of human colorectal cancer cells and their ability to adapt to apoptotic stimulus.

Zheng HY, Zhang XY, Wang XF, Sun BC - Cancer Biol Med (2012)

LC3B protein was detected in human colorectal cancer tissues by immunohistochemical staining with LC3B antibody. A: LC3B expression in cancer cells showing equal or stronger intensity than in nerve cells (arrow) were deemed “strongly positive.” B: LC3B expression in cancer cells showing weaker intensity than in nerve cells (arrow) were deemed “weakly positive”. Original magnification, × 40.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643655&req=5

f1: LC3B protein was detected in human colorectal cancer tissues by immunohistochemical staining with LC3B antibody. A: LC3B expression in cancer cells showing equal or stronger intensity than in nerve cells (arrow) were deemed “strongly positive.” B: LC3B expression in cancer cells showing weaker intensity than in nerve cells (arrow) were deemed “weakly positive”. Original magnification, × 40.
Mentions: The nerve plexus cells in the submucosal or muscularis portions of the colorectal wall showed constantly intensive staining for LC3B [19]. Thus, each section had its own positive internal control. On this basis, LC3B expression in tumor cells or in normal epithelia was compared with that in nerve cells of the same sections. The following subgroups of nerve cells were then identified: i) those with similar or enhanced LC3B expression in tumor cells or normal epithelia compared with those in nerve cells were judged as ‘‘strongly positive” (Figure 1A), and ii) those with weaker LC3B expression in tumor cells or normal epithelia than those in nerve cells were designated as ‘‘weakly positive’’ (Figure 1B). All immunostaining experiments were evaluated twice by 2 pathologists blinded to the patient outcome and other clinical findings.

Bottom Line: LC3B was expressed both in cancer cells and normal epithelial cells.LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002).Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China.

ABSTRACT

Objective: To investigate LC3B-II and active caspase-3 expression in human colorectal cancer to elucidate the role of autophagy, and to explore the relationship of autophagy with apoptosis in human colorectal cancer.

Methods: LC3B expression was detected by immunohistochemistry in 53 human colorectal cancer tissues and 20 normal colon tissues. The protein levels of LC3B-II and active caspase-3 were also determined by Western blot analysis in 23 human colorectal cancer tissues and 10 normal colon tissues.

Results: LC3B was expressed both in cancer cells and normal epithelial cells. LC3B expression in the peripheral area of cancer tissues was correlated with several clinicopathological factors, including tumor differentiation (P=0.002), growth pattern of the tumor margin (P=0.028), pN (P=0.002), pStage (P=0.032), as well as vessel and nerve plexus invasion (P=0.002). The protein level of LC3B-II in cancer tissue was significantly higher than in normal tissue (P=0.038), but the expression of active forms of procaspase-3 in cancer tissue was lower (P=0.041). There was a statistically significant positive correlation between the expression levels of LC3B-II and the active forms of procaspase-3 (r=0.537, P=0.008).

Conclusions: Autophagy has a prosurvival role in human colorectal cancer. Autophagy enhances the aggressiveness of colorectal cancer cells and their ability to adapt to apoptotic stimulus.

No MeSH data available.


Related in: MedlinePlus