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Expression of Bmi-1, P16, and CD44v6 in Uterine Cervical Carcinoma and Its Clinical Significance.

Weng MY, Li L, Feng SY, Hong SJ - Cancer Biol Med (2012)

Bottom Line: Nevertheless, the positive expression of p16 protein in UCC was not significantly associated with the clinicopathologic features (P>0.05).The Kaplan-Meier survival analysis showed that the over-expression of Bmi-1 significantly decreased the survival rate of UCC patients (P<0.05).The positive expression of p16 protein is possibly associated with the tumorigenesis, but not with the metastasis or prognosis of UCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, Sun Yat-sen Memorial Hospital of Zhongshan University, Guangzhou 510120, China.

ABSTRACT

Objective: Bmi-1, a putative proto-oncogene, is a core member of the polycomb gene family, which is expressed in many human tumors. The p16 protein negatively regulated cell proliferation, whereas CD44v6 is associated with proliferation as an important protein. Additionally, CD44v6 is an important nuclear antigen closely correlated to tumor metastasis. The present study aims to investigate the expression and significance of Bmi-1, p16, and CD44v6 in uterine cervical carcinoma (UCC).

Methods: A total of 62 UCC, 30 cervical neoplasic, and 20 normal cervical mucosal tissues were used in the current study. The expression of Bmi-1, p16, and CD44v6 in these tissues was determined using immunohistochemical assay. The relationships among the expression of these indices, the clinicopathologic features of UCC, and the survival rate of UCC patients were also discussed. The correlation between Bmi-1 protein expression and p16 or CD44v6 protein in UCC was analyzed.

Results: The expression of Bmi-1, p16, and CD44v6 was significantly high in cervical carcinoma compared with that in the cervical neoplasia and normal colorectal mucosa (P<0.05). The over-expression of Bmi-1 protein in UCC was apparently related to the distant metastasis (P<0.01) and the tumor, nodes and metastasis-classification, i.e. the TNM staging, World Health Organization (P<0.05). Nevertheless, the positive expression of p16 protein in UCC was not significantly associated with the clinicopathologic features (P>0.05). The Kaplan-Meier survival analysis showed that the over-expression of Bmi-1 significantly decreased the survival rate of UCC patients (P<0.05). A strong correlation indicated that there was statistical significance between the expression of Bmi-1 and CD44V6 proteins in UCC (r=0.419, P=0.001).

Conclusions: The over-expression of Bmi-1 and CD44v6 protein closely correlate to the tumorigenesis, metastasis, and prognosis of UCC. Bmi-1 and CD44v6 may be used to predict the prognosis of cervical carcinoma. Bmi-1 may indirectly regulate the expression of CD44v6 in UCC patients. The positive expression of p16 protein is possibly associated with the tumorigenesis, but not with the metastasis or prognosis of UCC.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical staining showed Bmi-1, P16, and CD44v6 protein expression in cervical mucous membrane. A, Bmi-1 protein; B, P16 protein; C, CD44v6 protein.
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f1: Immunohistochemical staining showed Bmi-1, P16, and CD44v6 protein expression in cervical mucous membrane. A, Bmi-1 protein; B, P16 protein; C, CD44v6 protein.

Mentions: The expression of Bmi-1 protein mainly presented as the pale or dark-brown particles in the nucleus. A few light-brown particles were also observed in the cytoplasm (Figure 1). In the present study, the positive rate of Bmi-1 was 54.8% for the 62 UCC cases (34/62), among which the over-expression rate was 29.0% (18/62). The over-expression rates of Bmi-1 for the 30 cases with cervical neoplasia and the 20 cases with normal cervical mucosa were 3.3% (1/30) and 0%, respectively. Thus, the expression of Bmi-1 protein was significantly higher in the UCC group than in the adenoma and normal controls (P<0.05). No statistically significant difference was observed between the protein expression in the adenoma and normal control groups (P>0.05). The positive coloration was indicated by the light-brown particles, with coloration of the cell membrane in most cells, and that of the cytoplasm in a few cells (Figure 1). For the normal cervical mucosa, cervical neoplasia, and UCC tissues, the positive expression rates of P16 protein were 5.0% (1/20), 6.7% (2/30), and 61.3% (38/62), respectively. Statistical significant differences were observed among the three. The p16 protein expression (Figure 1) in UCC was separately compared with that in cervical neoplasia and the normal cervical mucous membrane, showing significant differences in both comparisons (P<0.01). However, there was no statistical difference between the protein expression in cervical neoplasia and normal cervical mucosa (P>0.05). Among the 62 UCC cases in the present study, 14 were over-expressed in the expression of CD44v6 protein (Figure 1), showing an over-expression rate of 22.6% (14/62). In the 30 cases with cervical neoplasia, over-expression was found in only one case (severe atypical hyperplasia), with an over-expression rate of 3.33% (1/30). From the 20 cases with normal cervical mucosa, all were under-expressed. Thus, the expression of CD44v6 protein was obviously higher in the UCC than in the adenoma and normal control groups (P<0.05). No statistical difference was observed between the expression in the adenoma and normal control groups (P>0.05).


Expression of Bmi-1, P16, and CD44v6 in Uterine Cervical Carcinoma and Its Clinical Significance.

Weng MY, Li L, Feng SY, Hong SJ - Cancer Biol Med (2012)

Immunohistochemical staining showed Bmi-1, P16, and CD44v6 protein expression in cervical mucous membrane. A, Bmi-1 protein; B, P16 protein; C, CD44v6 protein.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643647&req=5

f1: Immunohistochemical staining showed Bmi-1, P16, and CD44v6 protein expression in cervical mucous membrane. A, Bmi-1 protein; B, P16 protein; C, CD44v6 protein.
Mentions: The expression of Bmi-1 protein mainly presented as the pale or dark-brown particles in the nucleus. A few light-brown particles were also observed in the cytoplasm (Figure 1). In the present study, the positive rate of Bmi-1 was 54.8% for the 62 UCC cases (34/62), among which the over-expression rate was 29.0% (18/62). The over-expression rates of Bmi-1 for the 30 cases with cervical neoplasia and the 20 cases with normal cervical mucosa were 3.3% (1/30) and 0%, respectively. Thus, the expression of Bmi-1 protein was significantly higher in the UCC group than in the adenoma and normal controls (P<0.05). No statistically significant difference was observed between the protein expression in the adenoma and normal control groups (P>0.05). The positive coloration was indicated by the light-brown particles, with coloration of the cell membrane in most cells, and that of the cytoplasm in a few cells (Figure 1). For the normal cervical mucosa, cervical neoplasia, and UCC tissues, the positive expression rates of P16 protein were 5.0% (1/20), 6.7% (2/30), and 61.3% (38/62), respectively. Statistical significant differences were observed among the three. The p16 protein expression (Figure 1) in UCC was separately compared with that in cervical neoplasia and the normal cervical mucous membrane, showing significant differences in both comparisons (P<0.01). However, there was no statistical difference between the protein expression in cervical neoplasia and normal cervical mucosa (P>0.05). Among the 62 UCC cases in the present study, 14 were over-expressed in the expression of CD44v6 protein (Figure 1), showing an over-expression rate of 22.6% (14/62). In the 30 cases with cervical neoplasia, over-expression was found in only one case (severe atypical hyperplasia), with an over-expression rate of 3.33% (1/30). From the 20 cases with normal cervical mucosa, all were under-expressed. Thus, the expression of CD44v6 protein was obviously higher in the UCC than in the adenoma and normal control groups (P<0.05). No statistical difference was observed between the expression in the adenoma and normal control groups (P>0.05).

Bottom Line: Nevertheless, the positive expression of p16 protein in UCC was not significantly associated with the clinicopathologic features (P>0.05).The Kaplan-Meier survival analysis showed that the over-expression of Bmi-1 significantly decreased the survival rate of UCC patients (P<0.05).The positive expression of p16 protein is possibly associated with the tumorigenesis, but not with the metastasis or prognosis of UCC.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology and Obstetrics, Sun Yat-sen Memorial Hospital of Zhongshan University, Guangzhou 510120, China.

ABSTRACT

Objective: Bmi-1, a putative proto-oncogene, is a core member of the polycomb gene family, which is expressed in many human tumors. The p16 protein negatively regulated cell proliferation, whereas CD44v6 is associated with proliferation as an important protein. Additionally, CD44v6 is an important nuclear antigen closely correlated to tumor metastasis. The present study aims to investigate the expression and significance of Bmi-1, p16, and CD44v6 in uterine cervical carcinoma (UCC).

Methods: A total of 62 UCC, 30 cervical neoplasic, and 20 normal cervical mucosal tissues were used in the current study. The expression of Bmi-1, p16, and CD44v6 in these tissues was determined using immunohistochemical assay. The relationships among the expression of these indices, the clinicopathologic features of UCC, and the survival rate of UCC patients were also discussed. The correlation between Bmi-1 protein expression and p16 or CD44v6 protein in UCC was analyzed.

Results: The expression of Bmi-1, p16, and CD44v6 was significantly high in cervical carcinoma compared with that in the cervical neoplasia and normal colorectal mucosa (P<0.05). The over-expression of Bmi-1 protein in UCC was apparently related to the distant metastasis (P<0.01) and the tumor, nodes and metastasis-classification, i.e. the TNM staging, World Health Organization (P<0.05). Nevertheless, the positive expression of p16 protein in UCC was not significantly associated with the clinicopathologic features (P>0.05). The Kaplan-Meier survival analysis showed that the over-expression of Bmi-1 significantly decreased the survival rate of UCC patients (P<0.05). A strong correlation indicated that there was statistical significance between the expression of Bmi-1 and CD44V6 proteins in UCC (r=0.419, P=0.001).

Conclusions: The over-expression of Bmi-1 and CD44v6 protein closely correlate to the tumorigenesis, metastasis, and prognosis of UCC. Bmi-1 and CD44v6 may be used to predict the prognosis of cervical carcinoma. Bmi-1 may indirectly regulate the expression of CD44v6 in UCC patients. The positive expression of p16 protein is possibly associated with the tumorigenesis, but not with the metastasis or prognosis of UCC.

No MeSH data available.


Related in: MedlinePlus