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Effect of Casilan(®) on (13)C-caffeine metabolism in overnight-fasted healthy Nigerian children.

Oshikoya KA, Smith K - J Pharmacol Pharmacother (2013)

Bottom Line: To determine the effect of Casilan(®) on (13)C-caffeine metabolism in healthy Nigerian children.This suggests that Casilan(®) did not affect the CYP1A2 metabolic pathway.Casilan(®) is a safe, reliable and quantitative food supplement for overnight-fasted children undergoing caffeine breath test.

View Article: PubMed Central - PubMed

Affiliation: Department of Child Health, Medical School in Derby, University of Nottingham, UK.

ABSTRACT

Objective: To determine the effect of Casilan(®) on (13)C-caffeine metabolism in healthy Nigerian children.

Materials and methods: TWELVE HEALTHY NIGERIAN CHILDREN (MALE: six, female: six) aged 3-8 years were studied on three occasions. After an overnight fast, the children were studied after ingesting Casilan(®) only (Week 1). They were restudied after ingesting 3 mg/kg of labeled caffeine only (Week 2), and further re-studied after ingesting both Casilan(®) and labeled caffeine (Week 3). Breath samples were collected by blowing via a straw into an exentainer bottle. The cumulative percentage of (13)C-caffeine exhaled as (13)CO2 was measured over 2 h.

Results: The time courses of (13)C-enrichments in exhaled CO2 for all the children, after they had ingested labeled caffeine only and after they had ingested both Casilan(®) and labeled caffeine, were identical. There was a gradual rise and peak of the enrichments at about 60-75 min, followed by a gradual fall (II) or a plateau (III). Contrarily, the time course of (13)C-enrichments for all the children was consistently low and stable after they had ingested Casilan(®) only (I). The mean values of cumulative percent (13)C-doses recovered in the CO2 exhaled over a 2-h period, after ingesting labeled caffeine only (8.59 ± 1.10 δ%/mg) and after ingesting both Casilan(®) and labeled caffeine (8.58 ± 1.33 δ%/mg), were identical, with no statistically significant difference (P = 0.972). This suggests that Casilan(®) did not affect the CYP1A2 metabolic pathway.

Conclusions: Casilan(®) is a safe, reliable and quantitative food supplement for overnight-fasted children undergoing caffeine breath test.

No MeSH data available.


Related in: MedlinePlus

Caffeine breath test following the ingestion of Casilan®, labeled caffeine and both Casilan® and labeled caffeine
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Figure 2: Caffeine breath test following the ingestion of Casilan®, labeled caffeine and both Casilan® and labeled caffeine

Mentions: The CBT of the children, after they ingested Casilan® or labeled caffeine only and after they ingested both Casilan® and caffeine, is presented in Figure 2. Data were expressed as mean ± SD. The mean values of the percentage caffeine dose exhaled over 2 h were identical after the children had ingested caffeine only (8.59 ± 1.10 δ6%/mg) and after they had ingested both Casilan® and labeled caffeine (8.58 ± 1.33 δ %/mg). There was no statistically significant difference in the mean values (P = 0.972) [Table 1]. However, after the children had ingested Casilan® only, the mean value of the percentage caffeine dose exhaled over 2 h (0.58 ± 1.39 δ %/mg) was significantly lower than the values after they had ingested caffeine only (P = 0.000) and after they had ingested both Casilan® and labeled caffeine (P = 0.000). The cumulative 13CO2 exhaled over a 2-h period is dependent on the 3-N-demethylation of caffeine. The very low level observed after the children had ingested Casilan® only and the identically high levels observed after the children had ingested caffeine only and after they had ingested both Casilan® and labeled caffeine would suggest that Casilan®, as a food supplement, did not significantly affect the CYP1A2 metabolic pathway.


Effect of Casilan(®) on (13)C-caffeine metabolism in overnight-fasted healthy Nigerian children.

Oshikoya KA, Smith K - J Pharmacol Pharmacother (2013)

Caffeine breath test following the ingestion of Casilan®, labeled caffeine and both Casilan® and labeled caffeine
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643338&req=5

Figure 2: Caffeine breath test following the ingestion of Casilan®, labeled caffeine and both Casilan® and labeled caffeine
Mentions: The CBT of the children, after they ingested Casilan® or labeled caffeine only and after they ingested both Casilan® and caffeine, is presented in Figure 2. Data were expressed as mean ± SD. The mean values of the percentage caffeine dose exhaled over 2 h were identical after the children had ingested caffeine only (8.59 ± 1.10 δ6%/mg) and after they had ingested both Casilan® and labeled caffeine (8.58 ± 1.33 δ %/mg). There was no statistically significant difference in the mean values (P = 0.972) [Table 1]. However, after the children had ingested Casilan® only, the mean value of the percentage caffeine dose exhaled over 2 h (0.58 ± 1.39 δ %/mg) was significantly lower than the values after they had ingested caffeine only (P = 0.000) and after they had ingested both Casilan® and labeled caffeine (P = 0.000). The cumulative 13CO2 exhaled over a 2-h period is dependent on the 3-N-demethylation of caffeine. The very low level observed after the children had ingested Casilan® only and the identically high levels observed after the children had ingested caffeine only and after they had ingested both Casilan® and labeled caffeine would suggest that Casilan®, as a food supplement, did not significantly affect the CYP1A2 metabolic pathway.

Bottom Line: To determine the effect of Casilan(®) on (13)C-caffeine metabolism in healthy Nigerian children.This suggests that Casilan(®) did not affect the CYP1A2 metabolic pathway.Casilan(®) is a safe, reliable and quantitative food supplement for overnight-fasted children undergoing caffeine breath test.

View Article: PubMed Central - PubMed

Affiliation: Department of Child Health, Medical School in Derby, University of Nottingham, UK.

ABSTRACT

Objective: To determine the effect of Casilan(®) on (13)C-caffeine metabolism in healthy Nigerian children.

Materials and methods: TWELVE HEALTHY NIGERIAN CHILDREN (MALE: six, female: six) aged 3-8 years were studied on three occasions. After an overnight fast, the children were studied after ingesting Casilan(®) only (Week 1). They were restudied after ingesting 3 mg/kg of labeled caffeine only (Week 2), and further re-studied after ingesting both Casilan(®) and labeled caffeine (Week 3). Breath samples were collected by blowing via a straw into an exentainer bottle. The cumulative percentage of (13)C-caffeine exhaled as (13)CO2 was measured over 2 h.

Results: The time courses of (13)C-enrichments in exhaled CO2 for all the children, after they had ingested labeled caffeine only and after they had ingested both Casilan(®) and labeled caffeine, were identical. There was a gradual rise and peak of the enrichments at about 60-75 min, followed by a gradual fall (II) or a plateau (III). Contrarily, the time course of (13)C-enrichments for all the children was consistently low and stable after they had ingested Casilan(®) only (I). The mean values of cumulative percent (13)C-doses recovered in the CO2 exhaled over a 2-h period, after ingesting labeled caffeine only (8.59 ± 1.10 δ%/mg) and after ingesting both Casilan(®) and labeled caffeine (8.58 ± 1.33 δ%/mg), were identical, with no statistically significant difference (P = 0.972). This suggests that Casilan(®) did not affect the CYP1A2 metabolic pathway.

Conclusions: Casilan(®) is a safe, reliable and quantitative food supplement for overnight-fasted children undergoing caffeine breath test.

No MeSH data available.


Related in: MedlinePlus