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Effect of Casilan(®) on (13)C-caffeine metabolism in overnight-fasted healthy Nigerian children.

Oshikoya KA, Smith K - J Pharmacol Pharmacother (2013)

Bottom Line: To determine the effect of Casilan(®) on (13)C-caffeine metabolism in healthy Nigerian children.This suggests that Casilan(®) did not affect the CYP1A2 metabolic pathway.Casilan(®) is a safe, reliable and quantitative food supplement for overnight-fasted children undergoing caffeine breath test.

View Article: PubMed Central - PubMed

Affiliation: Department of Child Health, Medical School in Derby, University of Nottingham, UK.

ABSTRACT

Objective: To determine the effect of Casilan(®) on (13)C-caffeine metabolism in healthy Nigerian children.

Materials and methods: TWELVE HEALTHY NIGERIAN CHILDREN (MALE: six, female: six) aged 3-8 years were studied on three occasions. After an overnight fast, the children were studied after ingesting Casilan(®) only (Week 1). They were restudied after ingesting 3 mg/kg of labeled caffeine only (Week 2), and further re-studied after ingesting both Casilan(®) and labeled caffeine (Week 3). Breath samples were collected by blowing via a straw into an exentainer bottle. The cumulative percentage of (13)C-caffeine exhaled as (13)CO2 was measured over 2 h.

Results: The time courses of (13)C-enrichments in exhaled CO2 for all the children, after they had ingested labeled caffeine only and after they had ingested both Casilan(®) and labeled caffeine, were identical. There was a gradual rise and peak of the enrichments at about 60-75 min, followed by a gradual fall (II) or a plateau (III). Contrarily, the time course of (13)C-enrichments for all the children was consistently low and stable after they had ingested Casilan(®) only (I). The mean values of cumulative percent (13)C-doses recovered in the CO2 exhaled over a 2-h period, after ingesting labeled caffeine only (8.59 ± 1.10 δ%/mg) and after ingesting both Casilan(®) and labeled caffeine (8.58 ± 1.33 δ%/mg), were identical, with no statistically significant difference (P = 0.972). This suggests that Casilan(®) did not affect the CYP1A2 metabolic pathway.

Conclusions: Casilan(®) is a safe, reliable and quantitative food supplement for overnight-fasted children undergoing caffeine breath test.

No MeSH data available.


Related in: MedlinePlus

Time course of 13C-enrichment for normal healthy Nigerian children after ingesting Casilan® or labeled caffeine only, and after ingesting both Casilan® and caffeine
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Figure 1: Time course of 13C-enrichment for normal healthy Nigerian children after ingesting Casilan® or labeled caffeine only, and after ingesting both Casilan® and caffeine

Mentions: The average 13C-enrichment for the 12 children was calculated at each time the breath samples were collected over a 2-h study period. The average 13C-enrichments were determined for the children after they had ingested Casilan® only (study I), after they had ingested labeled caffeine only (study II) and after they had also ingested both Casilan® and labeled caffeine (study III). The time courses of 13C enrichments for the children, at different stages of the study (I-III), are shown in Figure 1. The curves differed according to the intake of a food supplement, i.e. the children had either ingested or not ingested Casilan® (a food supplement). After ingesting Casilan® only, the 13C enrichments in the exhaled CO2 were consistently low, fluctuating between ±10-3 delta%, and yielded almost a plateau line [I in Figure 1]. This suggests a low level of 13C in the Casilan® . The 13C enrichments in the exhaled CO2 of the children were identical after they had ingested labeled caffeine only and after they had ingested both Casilan® and labeled caffeine. The 13C-enrichments followed a typical time course for CBT. The values increased gradually, reaching their peak values at about 60–75 min [II and III in Figure 1], followed by a gradual fall [II in Figure 1]. However, the 13C enrichments approached a plateau after attaining the peak value in children who ingested both Casilan® and caffeine [III of Figure 1].


Effect of Casilan(®) on (13)C-caffeine metabolism in overnight-fasted healthy Nigerian children.

Oshikoya KA, Smith K - J Pharmacol Pharmacother (2013)

Time course of 13C-enrichment for normal healthy Nigerian children after ingesting Casilan® or labeled caffeine only, and after ingesting both Casilan® and caffeine
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3643338&req=5

Figure 1: Time course of 13C-enrichment for normal healthy Nigerian children after ingesting Casilan® or labeled caffeine only, and after ingesting both Casilan® and caffeine
Mentions: The average 13C-enrichment for the 12 children was calculated at each time the breath samples were collected over a 2-h study period. The average 13C-enrichments were determined for the children after they had ingested Casilan® only (study I), after they had ingested labeled caffeine only (study II) and after they had also ingested both Casilan® and labeled caffeine (study III). The time courses of 13C enrichments for the children, at different stages of the study (I-III), are shown in Figure 1. The curves differed according to the intake of a food supplement, i.e. the children had either ingested or not ingested Casilan® (a food supplement). After ingesting Casilan® only, the 13C enrichments in the exhaled CO2 were consistently low, fluctuating between ±10-3 delta%, and yielded almost a plateau line [I in Figure 1]. This suggests a low level of 13C in the Casilan® . The 13C enrichments in the exhaled CO2 of the children were identical after they had ingested labeled caffeine only and after they had ingested both Casilan® and labeled caffeine. The 13C-enrichments followed a typical time course for CBT. The values increased gradually, reaching their peak values at about 60–75 min [II and III in Figure 1], followed by a gradual fall [II in Figure 1]. However, the 13C enrichments approached a plateau after attaining the peak value in children who ingested both Casilan® and caffeine [III of Figure 1].

Bottom Line: To determine the effect of Casilan(®) on (13)C-caffeine metabolism in healthy Nigerian children.This suggests that Casilan(®) did not affect the CYP1A2 metabolic pathway.Casilan(®) is a safe, reliable and quantitative food supplement for overnight-fasted children undergoing caffeine breath test.

View Article: PubMed Central - PubMed

Affiliation: Department of Child Health, Medical School in Derby, University of Nottingham, UK.

ABSTRACT

Objective: To determine the effect of Casilan(®) on (13)C-caffeine metabolism in healthy Nigerian children.

Materials and methods: TWELVE HEALTHY NIGERIAN CHILDREN (MALE: six, female: six) aged 3-8 years were studied on three occasions. After an overnight fast, the children were studied after ingesting Casilan(®) only (Week 1). They were restudied after ingesting 3 mg/kg of labeled caffeine only (Week 2), and further re-studied after ingesting both Casilan(®) and labeled caffeine (Week 3). Breath samples were collected by blowing via a straw into an exentainer bottle. The cumulative percentage of (13)C-caffeine exhaled as (13)CO2 was measured over 2 h.

Results: The time courses of (13)C-enrichments in exhaled CO2 for all the children, after they had ingested labeled caffeine only and after they had ingested both Casilan(®) and labeled caffeine, were identical. There was a gradual rise and peak of the enrichments at about 60-75 min, followed by a gradual fall (II) or a plateau (III). Contrarily, the time course of (13)C-enrichments for all the children was consistently low and stable after they had ingested Casilan(®) only (I). The mean values of cumulative percent (13)C-doses recovered in the CO2 exhaled over a 2-h period, after ingesting labeled caffeine only (8.59 ± 1.10 δ%/mg) and after ingesting both Casilan(®) and labeled caffeine (8.58 ± 1.33 δ%/mg), were identical, with no statistically significant difference (P = 0.972). This suggests that Casilan(®) did not affect the CYP1A2 metabolic pathway.

Conclusions: Casilan(®) is a safe, reliable and quantitative food supplement for overnight-fasted children undergoing caffeine breath test.

No MeSH data available.


Related in: MedlinePlus