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Evaluation of applicability of the Sartorius Airport MD8 sampler for detection of Bacillus endospores in indoor air.

Lewandowski R, Kozłowska K, Szpakowska M, Trafny EA - Environ Monit Assess (2012)

Bottom Line: Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with the ambient room air.The microorganisms present in the ambient room air interfere with precise quantification of Bacillus endospores when their concentration is relatively low.The results of this study may be helpful in critical assessment of the results obtained from monitoring the air for bacterial endospores.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland.

ABSTRACT
This study was designed to evaluate the measuring range and lowest limit of detection of Bacillus endospores in the ambient room air when the Sartorius MD8 sampler, and two different culture methods for bacterial enumeration were used. Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with the ambient room air. The detection of endospores in the HEPA-filtered air was achievable: (1) when they were aerosolized at a concentration above 7.56 × 10(3) CFU/m(3) and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 4.00 × 10(2) CFU/m(3) and analyzed with pour plate method. The detection of endospores in the ambient room air was possible: (1) when they were aerosolized at a concentration above 9.1 × 10(3) CFU/m(3) and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 5.6 × 10(2) CFU/m(3) and analyzed with pour plate method. The microorganisms present in the ambient room air interfere with precise quantification of Bacillus endospores when their concentration is relatively low. The results of this study may be helpful in critical assessment of the results obtained from monitoring the air for bacterial endospores.

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The correlation between the numbers of B. atrophaeus spores collected using Airport MD8 sampler and the total number of spores aerosolized within the test chamber filled either with the ambient air (square) or with the HEPA-filtered air (circle). The culturable bacteria were enumerated using the pour plate method. Data are shown as the mean log values (±SD) per cubic meter of air. Linear regression coefficients (R2) are shown beside the each graph
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Fig3: The correlation between the numbers of B. atrophaeus spores collected using Airport MD8 sampler and the total number of spores aerosolized within the test chamber filled either with the ambient air (square) or with the HEPA-filtered air (circle). The culturable bacteria were enumerated using the pour plate method. Data are shown as the mean log values (±SD) per cubic meter of air. Linear regression coefficients (R2) are shown beside the each graph

Mentions: When the Airport MD8 sampler was used to collect bioaerosol samples and the pour plate method was employed for analysis of the number of culturable bacteria in the samples, a high correlation (R2 = 0.92) was observed between the number of aerosolized endospores and the number of the bacteria detected (Fig. 3). However, it holds true only when the experimental chamber was filled with HEPA-filtered air. When the sampling and analysis was made in the ambient air, the linear regression coefficient was significantly lower (R2 = 0.76). Therefore, the presence of other organisms and dust particles in ambient air might interfere with Bacillus endospores collection in gelatin membrane filters and their enumeration with microbial culture techniques.Fig. 3


Evaluation of applicability of the Sartorius Airport MD8 sampler for detection of Bacillus endospores in indoor air.

Lewandowski R, Kozłowska K, Szpakowska M, Trafny EA - Environ Monit Assess (2012)

The correlation between the numbers of B. atrophaeus spores collected using Airport MD8 sampler and the total number of spores aerosolized within the test chamber filled either with the ambient air (square) or with the HEPA-filtered air (circle). The culturable bacteria were enumerated using the pour plate method. Data are shown as the mean log values (±SD) per cubic meter of air. Linear regression coefficients (R2) are shown beside the each graph
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585950&req=5

Fig3: The correlation between the numbers of B. atrophaeus spores collected using Airport MD8 sampler and the total number of spores aerosolized within the test chamber filled either with the ambient air (square) or with the HEPA-filtered air (circle). The culturable bacteria were enumerated using the pour plate method. Data are shown as the mean log values (±SD) per cubic meter of air. Linear regression coefficients (R2) are shown beside the each graph
Mentions: When the Airport MD8 sampler was used to collect bioaerosol samples and the pour plate method was employed for analysis of the number of culturable bacteria in the samples, a high correlation (R2 = 0.92) was observed between the number of aerosolized endospores and the number of the bacteria detected (Fig. 3). However, it holds true only when the experimental chamber was filled with HEPA-filtered air. When the sampling and analysis was made in the ambient air, the linear regression coefficient was significantly lower (R2 = 0.76). Therefore, the presence of other organisms and dust particles in ambient air might interfere with Bacillus endospores collection in gelatin membrane filters and their enumeration with microbial culture techniques.Fig. 3

Bottom Line: Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with the ambient room air.The microorganisms present in the ambient room air interfere with precise quantification of Bacillus endospores when their concentration is relatively low.The results of this study may be helpful in critical assessment of the results obtained from monitoring the air for bacterial endospores.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland.

ABSTRACT
This study was designed to evaluate the measuring range and lowest limit of detection of Bacillus endospores in the ambient room air when the Sartorius MD8 sampler, and two different culture methods for bacterial enumeration were used. Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with the ambient room air. The detection of endospores in the HEPA-filtered air was achievable: (1) when they were aerosolized at a concentration above 7.56 × 10(3) CFU/m(3) and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 4.00 × 10(2) CFU/m(3) and analyzed with pour plate method. The detection of endospores in the ambient room air was possible: (1) when they were aerosolized at a concentration above 9.1 × 10(3) CFU/m(3) and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 5.6 × 10(2) CFU/m(3) and analyzed with pour plate method. The microorganisms present in the ambient room air interfere with precise quantification of Bacillus endospores when their concentration is relatively low. The results of this study may be helpful in critical assessment of the results obtained from monitoring the air for bacterial endospores.

Show MeSH
Related in: MedlinePlus