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Evaluation of applicability of the Sartorius Airport MD8 sampler for detection of Bacillus endospores in indoor air.

Lewandowski R, Kozłowska K, Szpakowska M, Trafny EA - Environ Monit Assess (2012)

Bottom Line: Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with the ambient room air.The microorganisms present in the ambient room air interfere with precise quantification of Bacillus endospores when their concentration is relatively low.The results of this study may be helpful in critical assessment of the results obtained from monitoring the air for bacterial endospores.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland.

ABSTRACT
This study was designed to evaluate the measuring range and lowest limit of detection of Bacillus endospores in the ambient room air when the Sartorius MD8 sampler, and two different culture methods for bacterial enumeration were used. Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with the ambient room air. The detection of endospores in the HEPA-filtered air was achievable: (1) when they were aerosolized at a concentration above 7.56 × 10(3) CFU/m(3) and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 4.00 × 10(2) CFU/m(3) and analyzed with pour plate method. The detection of endospores in the ambient room air was possible: (1) when they were aerosolized at a concentration above 9.1 × 10(3) CFU/m(3) and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 5.6 × 10(2) CFU/m(3) and analyzed with pour plate method. The microorganisms present in the ambient room air interfere with precise quantification of Bacillus endospores when their concentration is relatively low. The results of this study may be helpful in critical assessment of the results obtained from monitoring the air for bacterial endospores.

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Comparison of the number of culturable microorganisms in the samples of ambient air collected using the following air samplers: MAS100 (light gray bars), six-stage Andersen impactor (dark gray bars), and Airport MD8 (black bars) in the laboratory room. The bars are shown as the log values (light or dark gray bars) or as the mean log values (±SD) (black bars)
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Fig2: Comparison of the number of culturable microorganisms in the samples of ambient air collected using the following air samplers: MAS100 (light gray bars), six-stage Andersen impactor (dark gray bars), and Airport MD8 (black bars) in the laboratory room. The bars are shown as the log values (light or dark gray bars) or as the mean log values (±SD) (black bars)

Mentions: Every time before start of the aerosolization of B. atrophaeus endospores in the test chamber filled with the ambient room air, the air samples were taken outside the chamber for microbiological analysis. A total of three samples were collected per sampling event with use of the three air samplers (Airport MD8, the six-stage Andersen impactor, and MAS-100), as well as three different volumes of air samples (0.1, 0.5, and 1.0 m3) were taken. As it was shown in Fig. 2, the mean number of culturable microorganisms collected using the Airport MD8 sampler was similar when the volume of the air sampled were 0.1, 0.5, and 1.0 m3, and was equal to 4.0 × 102 (±1.73 × 102), 4.02 × 102 (±1.84 × 102), and 3.24 × 102 (±3.91 × 102) CFU/m3, respectively. The number of microorganisms recovered with use of MAS100 was comparable to the number of microorganisms collected with the six-stage Andersen.Fig. 2


Evaluation of applicability of the Sartorius Airport MD8 sampler for detection of Bacillus endospores in indoor air.

Lewandowski R, Kozłowska K, Szpakowska M, Trafny EA - Environ Monit Assess (2012)

Comparison of the number of culturable microorganisms in the samples of ambient air collected using the following air samplers: MAS100 (light gray bars), six-stage Andersen impactor (dark gray bars), and Airport MD8 (black bars) in the laboratory room. The bars are shown as the log values (light or dark gray bars) or as the mean log values (±SD) (black bars)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585950&req=5

Fig2: Comparison of the number of culturable microorganisms in the samples of ambient air collected using the following air samplers: MAS100 (light gray bars), six-stage Andersen impactor (dark gray bars), and Airport MD8 (black bars) in the laboratory room. The bars are shown as the log values (light or dark gray bars) or as the mean log values (±SD) (black bars)
Mentions: Every time before start of the aerosolization of B. atrophaeus endospores in the test chamber filled with the ambient room air, the air samples were taken outside the chamber for microbiological analysis. A total of three samples were collected per sampling event with use of the three air samplers (Airport MD8, the six-stage Andersen impactor, and MAS-100), as well as three different volumes of air samples (0.1, 0.5, and 1.0 m3) were taken. As it was shown in Fig. 2, the mean number of culturable microorganisms collected using the Airport MD8 sampler was similar when the volume of the air sampled were 0.1, 0.5, and 1.0 m3, and was equal to 4.0 × 102 (±1.73 × 102), 4.02 × 102 (±1.84 × 102), and 3.24 × 102 (±3.91 × 102) CFU/m3, respectively. The number of microorganisms recovered with use of MAS100 was comparable to the number of microorganisms collected with the six-stage Andersen.Fig. 2

Bottom Line: Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with the ambient room air.The microorganisms present in the ambient room air interfere with precise quantification of Bacillus endospores when their concentration is relatively low.The results of this study may be helpful in critical assessment of the results obtained from monitoring the air for bacterial endospores.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Military Institute of Hygiene and Epidemiology, Kozielska 4, 01-163 Warsaw, Poland.

ABSTRACT
This study was designed to evaluate the measuring range and lowest limit of detection of Bacillus endospores in the ambient room air when the Sartorius MD8 sampler, and two different culture methods for bacterial enumeration were used. Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with the ambient room air. The detection of endospores in the HEPA-filtered air was achievable: (1) when they were aerosolized at a concentration above 7.56 × 10(3) CFU/m(3) and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 4.00 × 10(2) CFU/m(3) and analyzed with pour plate method. The detection of endospores in the ambient room air was possible: (1) when they were aerosolized at a concentration above 9.1 × 10(3) CFU/m(3) and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 5.6 × 10(2) CFU/m(3) and analyzed with pour plate method. The microorganisms present in the ambient room air interfere with precise quantification of Bacillus endospores when their concentration is relatively low. The results of this study may be helpful in critical assessment of the results obtained from monitoring the air for bacterial endospores.

Show MeSH