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Gene expression profile analysis of type 2 diabetic mouse liver.

Zhang F, Xu X, Zhang Y, Zhou B, He Z, Zhai Q - PLoS ONE (2013)

Bottom Line: Analysis of the key enzyme genes in fatty acid and glucose metabolism showed that some key enzyme genes were significantly increased and none of the detected key enzyme genes were decreased.In addition, FunDo analysis showed that liver cancer and hepatitis were most likely to be associated with diabetes.Taken together, this study provides the digital gene expression profile of diabetic mouse liver, and demonstrates the main diabetes-associated hepatic biological processes, pathways, key enzyme genes in fatty acid and glucose metabolism and potential hepatic diseases.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

ABSTRACT
Liver plays a key role in glucose metabolism and homeostasis, and impaired hepatic glucose metabolism contributes to the development of type 2 diabetes. However, the precise gene expression profile of diabetic liver and its association with diabetes and related diseases are yet to be further elucidated. In this study, we detected the gene expression profile by high-throughput sequencing in 9-week-old normal and type 2 diabetic db/db mouse liver. Totally 12132 genes were detected, and 2627 genes were significantly changed in diabetic mouse liver. Biological process analysis showed that the upregulated genes in diabetic mouse liver were mainly enriched in metabolic processes. Surprisingly, the downregulated genes in diabetic mouse liver were mainly enriched in immune-related processes, although all the altered genes were still mainly enriched in metabolic processes. Similarly, KEGG pathway analysis showed that metabolic pathways were the major pathways altered in diabetic mouse liver, and downregulated genes were enriched in immune and cancer pathways. Analysis of the key enzyme genes in fatty acid and glucose metabolism showed that some key enzyme genes were significantly increased and none of the detected key enzyme genes were decreased. In addition, FunDo analysis showed that liver cancer and hepatitis were most likely to be associated with diabetes. Taken together, this study provides the digital gene expression profile of diabetic mouse liver, and demonstrates the main diabetes-associated hepatic biological processes, pathways, key enzyme genes in fatty acid and glucose metabolism and potential hepatic diseases.

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Diabetes is correlated with different liver diseases at a transcriptional view.(A) The map of top 5 liver diseases enriched with the genes altered in 9-week-old db/db mouse liver. 2627 altered genes were assigned to different diseases using the web tool FunDO. The sizes of the disease nodes are proportional to the number of enriched genes. (B) The number of hit genes and P-value of the top 5 enriched liver diseases in (A).
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pone-0057766-g006: Diabetes is correlated with different liver diseases at a transcriptional view.(A) The map of top 5 liver diseases enriched with the genes altered in 9-week-old db/db mouse liver. 2627 altered genes were assigned to different diseases using the web tool FunDO. The sizes of the disease nodes are proportional to the number of enriched genes. (B) The number of hit genes and P-value of the top 5 enriched liver diseases in (A).

Mentions: To further elucidate the correlations between diabetes and hepatic diseases, we assigned the differentially expressed genes in the diabetic mouse liver to different diseases using web tool FunDO. As shown in Figure 6, 2627 differentially expressed genes were mainly related with liver cancer, hepatitis, liver disease, adenovirus infection and liver tumor. These results are in line with the correlation of diabetes with immunity and cancer [30], [31], [32].


Gene expression profile analysis of type 2 diabetic mouse liver.

Zhang F, Xu X, Zhang Y, Zhou B, He Z, Zhai Q - PLoS ONE (2013)

Diabetes is correlated with different liver diseases at a transcriptional view.(A) The map of top 5 liver diseases enriched with the genes altered in 9-week-old db/db mouse liver. 2627 altered genes were assigned to different diseases using the web tool FunDO. The sizes of the disease nodes are proportional to the number of enriched genes. (B) The number of hit genes and P-value of the top 5 enriched liver diseases in (A).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585940&req=5

pone-0057766-g006: Diabetes is correlated with different liver diseases at a transcriptional view.(A) The map of top 5 liver diseases enriched with the genes altered in 9-week-old db/db mouse liver. 2627 altered genes were assigned to different diseases using the web tool FunDO. The sizes of the disease nodes are proportional to the number of enriched genes. (B) The number of hit genes and P-value of the top 5 enriched liver diseases in (A).
Mentions: To further elucidate the correlations between diabetes and hepatic diseases, we assigned the differentially expressed genes in the diabetic mouse liver to different diseases using web tool FunDO. As shown in Figure 6, 2627 differentially expressed genes were mainly related with liver cancer, hepatitis, liver disease, adenovirus infection and liver tumor. These results are in line with the correlation of diabetes with immunity and cancer [30], [31], [32].

Bottom Line: Analysis of the key enzyme genes in fatty acid and glucose metabolism showed that some key enzyme genes were significantly increased and none of the detected key enzyme genes were decreased.In addition, FunDo analysis showed that liver cancer and hepatitis were most likely to be associated with diabetes.Taken together, this study provides the digital gene expression profile of diabetic mouse liver, and demonstrates the main diabetes-associated hepatic biological processes, pathways, key enzyme genes in fatty acid and glucose metabolism and potential hepatic diseases.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

ABSTRACT
Liver plays a key role in glucose metabolism and homeostasis, and impaired hepatic glucose metabolism contributes to the development of type 2 diabetes. However, the precise gene expression profile of diabetic liver and its association with diabetes and related diseases are yet to be further elucidated. In this study, we detected the gene expression profile by high-throughput sequencing in 9-week-old normal and type 2 diabetic db/db mouse liver. Totally 12132 genes were detected, and 2627 genes were significantly changed in diabetic mouse liver. Biological process analysis showed that the upregulated genes in diabetic mouse liver were mainly enriched in metabolic processes. Surprisingly, the downregulated genes in diabetic mouse liver were mainly enriched in immune-related processes, although all the altered genes were still mainly enriched in metabolic processes. Similarly, KEGG pathway analysis showed that metabolic pathways were the major pathways altered in diabetic mouse liver, and downregulated genes were enriched in immune and cancer pathways. Analysis of the key enzyme genes in fatty acid and glucose metabolism showed that some key enzyme genes were significantly increased and none of the detected key enzyme genes were decreased. In addition, FunDo analysis showed that liver cancer and hepatitis were most likely to be associated with diabetes. Taken together, this study provides the digital gene expression profile of diabetic mouse liver, and demonstrates the main diabetes-associated hepatic biological processes, pathways, key enzyme genes in fatty acid and glucose metabolism and potential hepatic diseases.

Show MeSH
Related in: MedlinePlus