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Diurnal-and sex-related difference of metallothionein expression in mice.

Zhang D, Jin T, Xu YQ, Lu YF, Wu Q, Zhang YK, Liu J - J Circadian Rhythms (2012)

Bottom Line: The diurnal variation of MT mRNAs resembled the clock gene albumin site D-binding protein (Dbp), and was anti-phase to the clock gene Brain and Muscle ARNT-like Protein 1 (Bmal1) in liver and kidneys.The peaks of MT mRNA levels were higher in females than in males.Hepatic MT protein followed a similar pattern, with about a 3-fold difference.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dept of Pharmacology and Key Lab of Basic Pharmacology of Guizhou, Zunyi Medical College, Zunyi, Guizhou 563003, China. JLiu@kumc.edu.

ABSTRACT

Background: Metallothionein (MT) is a small, cysteine-rich, metal-binding protein that plays an important role in protecting against toxicity of heavy metal and chemicals. This study was aimed to define diurnal and sex variation of MT in mice.

Methods: Adult mice were maintained in light- and temperature-controlled facilities for 2 weeks with light on at 8:00 and light off at 20:00. The blood, liver, and kidneys were collected every 4 h during the 24 h period. Total RNA was isolated, purified, and subjected to real-time RT-PCR analysis and MT protein was determined by western blot and the Cd/hemoglobin assay.

Results: The diurnal variations in mRNA levels of MT-1 and MT-2in liver were dramatic, up to a 40-foldpeak/trough ratio. MT mRNA levels in kidneys and blood also showed diurnal variation, up to 5-fold peak/trough ratio. The diurnal variation of MT mRNAs resembled the clock gene albumin site D-binding protein (Dbp), and was anti-phase to the clock gene Brain and Muscle ARNT-like Protein 1 (Bmal1) in liver and kidneys. The peaks of MT mRNA levels were higher in females than in males. Hepatic MT protein followed a similar pattern, with about a 3-fold difference.

Conclusion: MT mRNA levels and protein showed diurnal- and sex-variation in liver, kidney, and blood of mice, which could impact the body defense against toxic stimuli.

No MeSH data available.


Related in: MedlinePlus

Diurnal variations of mRNA levels of the clock gene Bmal1 in adult female and male KM mouse livers and kidneys (n = 4/sex/time point). Values are means ± SEM. Circadian (t = 24 h) rhythms was confirmed by the cosine algorithm method (p < 0.05).
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Figure 1: Diurnal variations of mRNA levels of the clock gene Bmal1 in adult female and male KM mouse livers and kidneys (n = 4/sex/time point). Values are means ± SEM. Circadian (t = 24 h) rhythms was confirmed by the cosine algorithm method (p < 0.05).

Mentions: To verify the circadian clock gene expression pattern in KM mice, we analyzed the clock gene Bmal1 in livers and kidneys of KM mice using real-time quantitative RT-PCR. The expression of Bmal1 had a nadir at 18:00 and a peak at 6:00. The differences of the nadirs and peaks were 14-fold for females and 54-fold for males in liver; while in kidneys were 26-fold for females and 37.5-fold for males. Statistically significant 24 h rhythms were confirmed by cosine algorithm method (p < 0.05) (Figure 1).


Diurnal-and sex-related difference of metallothionein expression in mice.

Zhang D, Jin T, Xu YQ, Lu YF, Wu Q, Zhang YK, Liu J - J Circadian Rhythms (2012)

Diurnal variations of mRNA levels of the clock gene Bmal1 in adult female and male KM mouse livers and kidneys (n = 4/sex/time point). Values are means ± SEM. Circadian (t = 24 h) rhythms was confirmed by the cosine algorithm method (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585924&req=5

Figure 1: Diurnal variations of mRNA levels of the clock gene Bmal1 in adult female and male KM mouse livers and kidneys (n = 4/sex/time point). Values are means ± SEM. Circadian (t = 24 h) rhythms was confirmed by the cosine algorithm method (p < 0.05).
Mentions: To verify the circadian clock gene expression pattern in KM mice, we analyzed the clock gene Bmal1 in livers and kidneys of KM mice using real-time quantitative RT-PCR. The expression of Bmal1 had a nadir at 18:00 and a peak at 6:00. The differences of the nadirs and peaks were 14-fold for females and 54-fold for males in liver; while in kidneys were 26-fold for females and 37.5-fold for males. Statistically significant 24 h rhythms were confirmed by cosine algorithm method (p < 0.05) (Figure 1).

Bottom Line: The diurnal variation of MT mRNAs resembled the clock gene albumin site D-binding protein (Dbp), and was anti-phase to the clock gene Brain and Muscle ARNT-like Protein 1 (Bmal1) in liver and kidneys.The peaks of MT mRNA levels were higher in females than in males.Hepatic MT protein followed a similar pattern, with about a 3-fold difference.

View Article: PubMed Central - HTML - PubMed

Affiliation: Dept of Pharmacology and Key Lab of Basic Pharmacology of Guizhou, Zunyi Medical College, Zunyi, Guizhou 563003, China. JLiu@kumc.edu.

ABSTRACT

Background: Metallothionein (MT) is a small, cysteine-rich, metal-binding protein that plays an important role in protecting against toxicity of heavy metal and chemicals. This study was aimed to define diurnal and sex variation of MT in mice.

Methods: Adult mice were maintained in light- and temperature-controlled facilities for 2 weeks with light on at 8:00 and light off at 20:00. The blood, liver, and kidneys were collected every 4 h during the 24 h period. Total RNA was isolated, purified, and subjected to real-time RT-PCR analysis and MT protein was determined by western blot and the Cd/hemoglobin assay.

Results: The diurnal variations in mRNA levels of MT-1 and MT-2in liver were dramatic, up to a 40-foldpeak/trough ratio. MT mRNA levels in kidneys and blood also showed diurnal variation, up to 5-fold peak/trough ratio. The diurnal variation of MT mRNAs resembled the clock gene albumin site D-binding protein (Dbp), and was anti-phase to the clock gene Brain and Muscle ARNT-like Protein 1 (Bmal1) in liver and kidneys. The peaks of MT mRNA levels were higher in females than in males. Hepatic MT protein followed a similar pattern, with about a 3-fold difference.

Conclusion: MT mRNA levels and protein showed diurnal- and sex-variation in liver, kidney, and blood of mice, which could impact the body defense against toxic stimuli.

No MeSH data available.


Related in: MedlinePlus