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Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells.

Mussá T, Rodríguez-Cariño C, Sánchez-Chardi A, Baratelli M, Costa-Hurtado M, Fraile L, Domínguez J, Aragon V, Montoya M - Vet. Res. (2012)

Bottom Line: The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone.Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki.These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain. maria.montoya@cresa.uab.es.

ABSTRACT
Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial infections, including those by H. parasuis. However, the mechanisms involved in host innate responses towards H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki. These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.

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Cytokine secretion by poBMDC at 8 hpi. poBMDC were infected or co-infected for 8 h with H3N2 SwIV and SW114 or Nagasaki. Supernatants of mock, single or co-infection were analysed for cytokine secretion using ELISA. Bars are mean ± SEM of three independent experiments using poBMDC derived from three animals. The presence of significant differences (p < 0.05) between groups are represented by (a, b or c).
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Figure 9: Cytokine secretion by poBMDC at 8 hpi. poBMDC were infected or co-infected for 8 h with H3N2 SwIV and SW114 or Nagasaki. Supernatants of mock, single or co-infection were analysed for cytokine secretion using ELISA. Bars are mean ± SEM of three independent experiments using poBMDC derived from three animals. The presence of significant differences (p < 0.05) between groups are represented by (a, b or c).

Mentions: Once the profile of poBMDC activation after H. parasuis infection was characterised, we analysed the cytokine profile to determine the type of immune response that these pathogens may induce in poBMDC. Consequently, IL-1β, TNF-α, IL-6, IL-10, IL-8, IL-12, IL-18 and IFN-α were analysed at 8 hpi. Statistically significant differences (p < 0.05) were found in the secretion of IL-1β, IL-6, TNF-α and IL-10 between SW114 and Nagasaki-infected poBMDC compared to mock cells. IL-12 was secreted in higher levels by SW114-infected cells compared to Nagasaki-infected cells and to mock cells (Figure 9). No significant differences were found in IL-8, IL-18 or IFN-α secretion from SW114 or Nagasaki infected cells compared to the mock controls (Figure 9e, 9f and 9h).


Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells.

Mussá T, Rodríguez-Cariño C, Sánchez-Chardi A, Baratelli M, Costa-Hurtado M, Fraile L, Domínguez J, Aragon V, Montoya M - Vet. Res. (2012)

Cytokine secretion by poBMDC at 8 hpi. poBMDC were infected or co-infected for 8 h with H3N2 SwIV and SW114 or Nagasaki. Supernatants of mock, single or co-infection were analysed for cytokine secretion using ELISA. Bars are mean ± SEM of three independent experiments using poBMDC derived from three animals. The presence of significant differences (p < 0.05) between groups are represented by (a, b or c).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585918&req=5

Figure 9: Cytokine secretion by poBMDC at 8 hpi. poBMDC were infected or co-infected for 8 h with H3N2 SwIV and SW114 or Nagasaki. Supernatants of mock, single or co-infection were analysed for cytokine secretion using ELISA. Bars are mean ± SEM of three independent experiments using poBMDC derived from three animals. The presence of significant differences (p < 0.05) between groups are represented by (a, b or c).
Mentions: Once the profile of poBMDC activation after H. parasuis infection was characterised, we analysed the cytokine profile to determine the type of immune response that these pathogens may induce in poBMDC. Consequently, IL-1β, TNF-α, IL-6, IL-10, IL-8, IL-12, IL-18 and IFN-α were analysed at 8 hpi. Statistically significant differences (p < 0.05) were found in the secretion of IL-1β, IL-6, TNF-α and IL-10 between SW114 and Nagasaki-infected poBMDC compared to mock cells. IL-12 was secreted in higher levels by SW114-infected cells compared to Nagasaki-infected cells and to mock cells (Figure 9). No significant differences were found in IL-8, IL-18 or IFN-α secretion from SW114 or Nagasaki infected cells compared to the mock controls (Figure 9e, 9f and 9h).

Bottom Line: The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone.Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki.These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain. maria.montoya@cresa.uab.es.

ABSTRACT
Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial infections, including those by H. parasuis. However, the mechanisms involved in host innate responses towards H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki. These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.

Show MeSH
Related in: MedlinePlus