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Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells.

Mussá T, Rodríguez-Cariño C, Sánchez-Chardi A, Baratelli M, Costa-Hurtado M, Fraile L, Domínguez J, Aragon V, Montoya M - Vet. Res. (2012)

Bottom Line: The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone.Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki.These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain. maria.montoya@cresa.uab.es.

ABSTRACT
Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial infections, including those by H. parasuis. However, the mechanisms involved in host innate responses towards H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki. These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.

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Staining of H. parasuis SW114 or Nagasaki at 1 h (a) and 8h (b) after infection. Porcine BMDC were infected and stained using anti-SW14 or anti-Nagasaki rabbit serum for 1 h at 4°C, and then with the anti-rabbit IgG-FITC antibody. Mock (grey histograms), SW114 (dotted line), Nagasaki (continuous line). Representative of independent experiments using poBMDC derived from four animals.
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Figure 2: Staining of H. parasuis SW114 or Nagasaki at 1 h (a) and 8h (b) after infection. Porcine BMDC were infected and stained using anti-SW14 or anti-Nagasaki rabbit serum for 1 h at 4°C, and then with the anti-rabbit IgG-FITC antibody. Mock (grey histograms), SW114 (dotted line), Nagasaki (continuous line). Representative of independent experiments using poBMDC derived from four animals.

Mentions: Measuring H. parasuis interaction with swine DC may be a suitable indicator of virulence since DC ability to capture antigens is important to elicit a specific immune response. At 1 hpi poBMDC infected with SW114 or Nagasaki showed higher fluorescence intensity compared to mock cells (Figure 2a), however, at 8 hpi it seems that both strains interacted equally with poBMDC compared to the mock cells (Figure 2b).


Differential interactions of virulent and non-virulent H. parasuis strains with naïve or swine influenza virus pre-infected dendritic cells.

Mussá T, Rodríguez-Cariño C, Sánchez-Chardi A, Baratelli M, Costa-Hurtado M, Fraile L, Domínguez J, Aragon V, Montoya M - Vet. Res. (2012)

Staining of H. parasuis SW114 or Nagasaki at 1 h (a) and 8h (b) after infection. Porcine BMDC were infected and stained using anti-SW14 or anti-Nagasaki rabbit serum for 1 h at 4°C, and then with the anti-rabbit IgG-FITC antibody. Mock (grey histograms), SW114 (dotted line), Nagasaki (continuous line). Representative of independent experiments using poBMDC derived from four animals.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585918&req=5

Figure 2: Staining of H. parasuis SW114 or Nagasaki at 1 h (a) and 8h (b) after infection. Porcine BMDC were infected and stained using anti-SW14 or anti-Nagasaki rabbit serum for 1 h at 4°C, and then with the anti-rabbit IgG-FITC antibody. Mock (grey histograms), SW114 (dotted line), Nagasaki (continuous line). Representative of independent experiments using poBMDC derived from four animals.
Mentions: Measuring H. parasuis interaction with swine DC may be a suitable indicator of virulence since DC ability to capture antigens is important to elicit a specific immune response. At 1 hpi poBMDC infected with SW114 or Nagasaki showed higher fluorescence intensity compared to mock cells (Figure 2a), however, at 8 hpi it seems that both strains interacted equally with poBMDC compared to the mock cells (Figure 2b).

Bottom Line: The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone.Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki.These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.

View Article: PubMed Central - HTML - PubMed

Affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain. maria.montoya@cresa.uab.es.

ABSTRACT
Pigs possess a microbiota in the upper respiratory tract that includes Haemophilus parasuis. Pigs are also considered the reservoir of influenza viruses and infection with this virus commonly results in increased impact of bacterial infections, including those by H. parasuis. However, the mechanisms involved in host innate responses towards H. parasuis and their implications in a co-infection with influenza virus are unknown. Therefore, the ability of a non-virulent H. parasuis serovar 3 (SW114) and a virulent serovar 5 (Nagasaki) strains to interact with porcine bone marrow dendritic cells (poBMDC) and their modulation in a co-infection with swine influenza virus (SwIV) H3N2 was examined. At 1 hour post infection (hpi), SW114 interaction with poBMDC was higher than that of Nagasaki, while at 8 hpi both strains showed similar levels of interaction. The co-infection with H3N2 SwIV and either SW114 or Nagasaki induced higher levels of IL-1β, TNF-α, IL-6, IL-12 and IL-10 compared to mock or H3N2 SwIV infection alone. Moreover, IL-12 and IFN-α secretion differentially increased in cells co-infected with H3N2 SwIV and Nagasaki. These results pave the way for understanding the differences in the interaction of non-virulent and virulent strains of H. parasuis with the swine immune system and their modulation in a viral co-infection.

Show MeSH
Related in: MedlinePlus