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Visualizing the prostate gland by MR imaging in young and old mice.

Ravoori M, Duggal J, Gagea M, Han L, Singh S, Liu P, Wei W, Ragan DK, Bankson JA, Ma J, Kundra V - PLoS ONE (2013)

Bottom Line: Prostate volume and signal characteristics were compared and histology was performed.The decrease in T2 and T2-based Dixon "water only" signal with age corresponds with glandular hyperplasia.Findings suggest age should be an important determinant when choosing models of prostate biology and disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America.

ABSTRACT

Purpose: Prostate imaging requires optimization in young and old mouse models. We tested which MR sequences and field strengths best depict the prostate gland in young and old mice; and, whether prostate MR signal, size, and architecture change with age.

Technique: Magnetic resonance imaging (MRI) of the prostate of young (2 months) and old (18 months) male nude mice (n = 6) was performed at 4.7 and 7 T and SCID mice (n = 6) at 7 T field strengths, using T1, fat suppressed T1, DWI, T2, fat suppressed T2, as well as T2-based- and proton density-based Dixon "water only" sequences. Images were ranked for best overall sequence for prostate visualization, prostate delineation, and quality of fat suppression. Prostate volume and signal characteristics were compared and histology was performed.

Results: T2-based-Dixon "water only" images ranked best overall for prostate visualization and delineation as well as fat suppression (n = 6, P<0.001) at both 4.7 T and 7 T in nude and 7T in SCID mice. Evaluated in nude mice, T2-based Dixon "water only" had greater prostate CNR and lower fat SNR at 7 T than 4.7 T (P<0.001). Prostate volume was less in older than younger mice (n = 6, P<0.02 nude mice; n = 6, P<0.002 SCID mice). Prostate T2 FSE as well as proton density-based and T2-based-Dixon "water only" signal intensity was higher in younger than older mice (P<0.001 nude mice; P<0.01 SCID mice) both at 4.7 and 7 T. This corresponded to an increase in glandular hyperplasia in older mice by histology (P<0.01, n = 6).

Conclusion: T2-based Dixon "water only" images best depict the mouse prostate in young and old nude mice at 4.7 and 7 T. The mouse prostate decreases in size with age. The decrease in T2 and T2-based Dixon "water only" signal with age corresponds with glandular hyperplasia. Findings suggest age should be an important determinant when choosing models of prostate biology and disease.

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Prostate gland histology of young and old mice, H&E stain, ×400.A) Nude young mice, minimal hyperplasia, grade 1; B) Nude old mice, mild hyperplasia, grade 2; C) SCID young mice, minimal hyperplasia, grade 1; D) SCID old mice, moderate hyperplasia, grade 3.
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pone-0055746-g005: Prostate gland histology of young and old mice, H&E stain, ×400.A) Nude young mice, minimal hyperplasia, grade 1; B) Nude old mice, mild hyperplasia, grade 2; C) SCID young mice, minimal hyperplasia, grade 1; D) SCID old mice, moderate hyperplasia, grade 3.

Mentions: Histologic examination of the mice demonstrated that the degree of glandular hyperplasia of the prostate gland increased in older mice in comparison with younger mice in both nude (P≤0.003, Table 5, Fig. 5A & B) and SCID mice (P≤0.007, Table 5, Fig. 5C & D). Young nude mice had normal or only minimal hyperplasia of the prostate gland epithelium (an average score of 0.67 on a scale of 0 to 4, Table 5, Fig. 5A) in comparison with old nude mice that commonly had mild or moderate hyperplasia (an average score of 2.0, Table 5, Fig. 5B). A similar pattern of changes was observed in the SCID mice (Table 5, Fig. 5C & D). No neoplastic lesions were observed in any of the mice of this study. Visually, younger mice had more proteinaceous secretion within the lumen of prostate glands in comparison with older mice in both nude and SCID mice.


Visualizing the prostate gland by MR imaging in young and old mice.

Ravoori M, Duggal J, Gagea M, Han L, Singh S, Liu P, Wei W, Ragan DK, Bankson JA, Ma J, Kundra V - PLoS ONE (2013)

Prostate gland histology of young and old mice, H&E stain, ×400.A) Nude young mice, minimal hyperplasia, grade 1; B) Nude old mice, mild hyperplasia, grade 2; C) SCID young mice, minimal hyperplasia, grade 1; D) SCID old mice, moderate hyperplasia, grade 3.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585879&req=5

pone-0055746-g005: Prostate gland histology of young and old mice, H&E stain, ×400.A) Nude young mice, minimal hyperplasia, grade 1; B) Nude old mice, mild hyperplasia, grade 2; C) SCID young mice, minimal hyperplasia, grade 1; D) SCID old mice, moderate hyperplasia, grade 3.
Mentions: Histologic examination of the mice demonstrated that the degree of glandular hyperplasia of the prostate gland increased in older mice in comparison with younger mice in both nude (P≤0.003, Table 5, Fig. 5A & B) and SCID mice (P≤0.007, Table 5, Fig. 5C & D). Young nude mice had normal or only minimal hyperplasia of the prostate gland epithelium (an average score of 0.67 on a scale of 0 to 4, Table 5, Fig. 5A) in comparison with old nude mice that commonly had mild or moderate hyperplasia (an average score of 2.0, Table 5, Fig. 5B). A similar pattern of changes was observed in the SCID mice (Table 5, Fig. 5C & D). No neoplastic lesions were observed in any of the mice of this study. Visually, younger mice had more proteinaceous secretion within the lumen of prostate glands in comparison with older mice in both nude and SCID mice.

Bottom Line: Prostate volume and signal characteristics were compared and histology was performed.The decrease in T2 and T2-based Dixon "water only" signal with age corresponds with glandular hyperplasia.Findings suggest age should be an important determinant when choosing models of prostate biology and disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Diagnostic Imaging, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America.

ABSTRACT

Purpose: Prostate imaging requires optimization in young and old mouse models. We tested which MR sequences and field strengths best depict the prostate gland in young and old mice; and, whether prostate MR signal, size, and architecture change with age.

Technique: Magnetic resonance imaging (MRI) of the prostate of young (2 months) and old (18 months) male nude mice (n = 6) was performed at 4.7 and 7 T and SCID mice (n = 6) at 7 T field strengths, using T1, fat suppressed T1, DWI, T2, fat suppressed T2, as well as T2-based- and proton density-based Dixon "water only" sequences. Images were ranked for best overall sequence for prostate visualization, prostate delineation, and quality of fat suppression. Prostate volume and signal characteristics were compared and histology was performed.

Results: T2-based-Dixon "water only" images ranked best overall for prostate visualization and delineation as well as fat suppression (n = 6, P<0.001) at both 4.7 T and 7 T in nude and 7T in SCID mice. Evaluated in nude mice, T2-based Dixon "water only" had greater prostate CNR and lower fat SNR at 7 T than 4.7 T (P<0.001). Prostate volume was less in older than younger mice (n = 6, P<0.02 nude mice; n = 6, P<0.002 SCID mice). Prostate T2 FSE as well as proton density-based and T2-based-Dixon "water only" signal intensity was higher in younger than older mice (P<0.001 nude mice; P<0.01 SCID mice) both at 4.7 and 7 T. This corresponded to an increase in glandular hyperplasia in older mice by histology (P<0.01, n = 6).

Conclusion: T2-based Dixon "water only" images best depict the mouse prostate in young and old nude mice at 4.7 and 7 T. The mouse prostate decreases in size with age. The decrease in T2 and T2-based Dixon "water only" signal with age corresponds with glandular hyperplasia. Findings suggest age should be an important determinant when choosing models of prostate biology and disease.

Show MeSH
Related in: MedlinePlus