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Therapeutic effects of radix dipsaci, pyrola herb, and Cynomorium songaricum on bone metabolism of ovariectomized rats.

Liu M, Xiao GG, Rong P, Zhang Z, Dong J, Zhao H, Li H, Li Y, Pan J, Liu H, Wang W, Zha Q, Ju D - BMC Complement Altern Med (2012)

Bottom Line: Bone mass and bone turnover were analyzed by histomorphometry.Treatment with either RDD or PHD enhanced trabecular bone formation remarkably.Our study showed that RDD and PHD increased bone formation by stimulating overexpression of OPG and downregulation of RANKL in OB and bMSC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Basic Theory, China Academy of Chinese Medical Sciences, South Small Street 16, Dongzhimennei, Dongcheng District, Beijing 100700, Peoples Republic of ChineChina.

ABSTRACT

Background: The objective of this study was to evaluate the effects of herbal medicines, such as Radix Dipsaci (RDD), Pyrola Herb (PHD), and Cynomorium songaricum decoction (CSD), on osteoporotic rats induced by ovariectomy (OVX).

Methods: OVX or sham operations were performed on 69 virgin Wistar rats that were divided into six groups: sham (sham, n = 12), OVX control group (OVX, n = 12), and OVX rats with treatments (diethylstilbestrol, E2, n = 12; RDD, n = 11, PHD, n = 11, and CSD, n = 11). Non-surgical rats served as normal control (NC, n = 12). The treatments began four weeks after surgery and lasted for 12 weeks. Bone mass and bone turnover were analyzed by histomorphometry. Levels of protein expression and mRNA of OPG and RANKL in osteoblasts (OB) and bone marrow stromal cells (bMSC) were evaluated by immunohistochemistry and in situ hybridization.

Results: Compared to NC and sham rats, trabecular bone formation was significantly reduced in OVX rats, but restored in E2-treated rats. Treatment with either RDD or PHD enhanced trabecular bone formation remarkably. No significant change of bone formation was observed in CSD-treated rats. OPG expression of protein and mRNA was reduced significantly in OB and bMSC of OVX control rats. RANKL expression of protein and mRNA was increased significantly in OB and bMSC of OVX control rats. These effects were substantially reversed (increased in OPG and decreased in RANKL) by treatment with E2, RDD, or PHD in OB and bMSC of OVX rats. No significant changes in either OPG or RANKL expression were observed in OB and bMSC of OVX rats treated with CSD.

Conclusions: Our study showed that RDD and PHD increased bone formation by stimulating overexpression of OPG and downregulation of RANKL in OB and bMSC. This suggests that RDD and PHD may be used as alternative therapeutic agents for postmenopausal osteoporosis.

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Effects of RDD, PHD, and CSD on protein expression of OPG and RANKL. A) Immunohistochemical stain of OPG; B) Immunohistochemical stain of RANKL; C) Ratio of RANKL/OPG protein expression, RANKL and OPG protein expression data were obtained from panels A and B of this figure, see details in the Methods section of the text, (a, p < 0.01, b, p < 0.05). All the experiments were repeated three times.
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Figure 2: Effects of RDD, PHD, and CSD on protein expression of OPG and RANKL. A) Immunohistochemical stain of OPG; B) Immunohistochemical stain of RANKL; C) Ratio of RANKL/OPG protein expression, RANKL and OPG protein expression data were obtained from panels A and B of this figure, see details in the Methods section of the text, (a, p < 0.01, b, p < 0.05). All the experiments were repeated three times.

Mentions: To monitor bone remodeling activity in OVX rats treated with the herbs, we analyzed biomarker expression of bone remodeling using immunohistochemistry (Figures 2A, B). OPG expression was decreased significantly in either OB or bMSC of tibia from OVX rats compared to the sham group (p < 0.01, Figure 2A). Either E2 or PHD treatment caused overexpression of OPG in OB and bMSC of tibia from OVX rats compared to that from the OVX control group (p < 0.05, Figure 2A); however, treatment with either RDD or CSD had no significant effect on OPG expression (p > 0.05).


Therapeutic effects of radix dipsaci, pyrola herb, and Cynomorium songaricum on bone metabolism of ovariectomized rats.

Liu M, Xiao GG, Rong P, Zhang Z, Dong J, Zhao H, Li H, Li Y, Pan J, Liu H, Wang W, Zha Q, Ju D - BMC Complement Altern Med (2012)

Effects of RDD, PHD, and CSD on protein expression of OPG and RANKL. A) Immunohistochemical stain of OPG; B) Immunohistochemical stain of RANKL; C) Ratio of RANKL/OPG protein expression, RANKL and OPG protein expression data were obtained from panels A and B of this figure, see details in the Methods section of the text, (a, p < 0.01, b, p < 0.05). All the experiments were repeated three times.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585854&req=5

Figure 2: Effects of RDD, PHD, and CSD on protein expression of OPG and RANKL. A) Immunohistochemical stain of OPG; B) Immunohistochemical stain of RANKL; C) Ratio of RANKL/OPG protein expression, RANKL and OPG protein expression data were obtained from panels A and B of this figure, see details in the Methods section of the text, (a, p < 0.01, b, p < 0.05). All the experiments were repeated three times.
Mentions: To monitor bone remodeling activity in OVX rats treated with the herbs, we analyzed biomarker expression of bone remodeling using immunohistochemistry (Figures 2A, B). OPG expression was decreased significantly in either OB or bMSC of tibia from OVX rats compared to the sham group (p < 0.01, Figure 2A). Either E2 or PHD treatment caused overexpression of OPG in OB and bMSC of tibia from OVX rats compared to that from the OVX control group (p < 0.05, Figure 2A); however, treatment with either RDD or CSD had no significant effect on OPG expression (p > 0.05).

Bottom Line: Bone mass and bone turnover were analyzed by histomorphometry.Treatment with either RDD or PHD enhanced trabecular bone formation remarkably.Our study showed that RDD and PHD increased bone formation by stimulating overexpression of OPG and downregulation of RANKL in OB and bMSC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Basic Theory, China Academy of Chinese Medical Sciences, South Small Street 16, Dongzhimennei, Dongcheng District, Beijing 100700, Peoples Republic of ChineChina.

ABSTRACT

Background: The objective of this study was to evaluate the effects of herbal medicines, such as Radix Dipsaci (RDD), Pyrola Herb (PHD), and Cynomorium songaricum decoction (CSD), on osteoporotic rats induced by ovariectomy (OVX).

Methods: OVX or sham operations were performed on 69 virgin Wistar rats that were divided into six groups: sham (sham, n = 12), OVX control group (OVX, n = 12), and OVX rats with treatments (diethylstilbestrol, E2, n = 12; RDD, n = 11, PHD, n = 11, and CSD, n = 11). Non-surgical rats served as normal control (NC, n = 12). The treatments began four weeks after surgery and lasted for 12 weeks. Bone mass and bone turnover were analyzed by histomorphometry. Levels of protein expression and mRNA of OPG and RANKL in osteoblasts (OB) and bone marrow stromal cells (bMSC) were evaluated by immunohistochemistry and in situ hybridization.

Results: Compared to NC and sham rats, trabecular bone formation was significantly reduced in OVX rats, but restored in E2-treated rats. Treatment with either RDD or PHD enhanced trabecular bone formation remarkably. No significant change of bone formation was observed in CSD-treated rats. OPG expression of protein and mRNA was reduced significantly in OB and bMSC of OVX control rats. RANKL expression of protein and mRNA was increased significantly in OB and bMSC of OVX control rats. These effects were substantially reversed (increased in OPG and decreased in RANKL) by treatment with E2, RDD, or PHD in OB and bMSC of OVX rats. No significant changes in either OPG or RANKL expression were observed in OB and bMSC of OVX rats treated with CSD.

Conclusions: Our study showed that RDD and PHD increased bone formation by stimulating overexpression of OPG and downregulation of RANKL in OB and bMSC. This suggests that RDD and PHD may be used as alternative therapeutic agents for postmenopausal osteoporosis.

Show MeSH
Related in: MedlinePlus