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Therapeutic effects of radix dipsaci, pyrola herb, and Cynomorium songaricum on bone metabolism of ovariectomized rats.

Liu M, Xiao GG, Rong P, Zhang Z, Dong J, Zhao H, Li H, Li Y, Pan J, Liu H, Wang W, Zha Q, Ju D - BMC Complement Altern Med (2012)

Bottom Line: Bone mass and bone turnover were analyzed by histomorphometry.Treatment with either RDD or PHD enhanced trabecular bone formation remarkably.Our study showed that RDD and PHD increased bone formation by stimulating overexpression of OPG and downregulation of RANKL in OB and bMSC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Basic Theory, China Academy of Chinese Medical Sciences, South Small Street 16, Dongzhimennei, Dongcheng District, Beijing 100700, Peoples Republic of ChineChina.

ABSTRACT

Background: The objective of this study was to evaluate the effects of herbal medicines, such as Radix Dipsaci (RDD), Pyrola Herb (PHD), and Cynomorium songaricum decoction (CSD), on osteoporotic rats induced by ovariectomy (OVX).

Methods: OVX or sham operations were performed on 69 virgin Wistar rats that were divided into six groups: sham (sham, n = 12), OVX control group (OVX, n = 12), and OVX rats with treatments (diethylstilbestrol, E2, n = 12; RDD, n = 11, PHD, n = 11, and CSD, n = 11). Non-surgical rats served as normal control (NC, n = 12). The treatments began four weeks after surgery and lasted for 12 weeks. Bone mass and bone turnover were analyzed by histomorphometry. Levels of protein expression and mRNA of OPG and RANKL in osteoblasts (OB) and bone marrow stromal cells (bMSC) were evaluated by immunohistochemistry and in situ hybridization.

Results: Compared to NC and sham rats, trabecular bone formation was significantly reduced in OVX rats, but restored in E2-treated rats. Treatment with either RDD or PHD enhanced trabecular bone formation remarkably. No significant change of bone formation was observed in CSD-treated rats. OPG expression of protein and mRNA was reduced significantly in OB and bMSC of OVX control rats. RANKL expression of protein and mRNA was increased significantly in OB and bMSC of OVX control rats. These effects were substantially reversed (increased in OPG and decreased in RANKL) by treatment with E2, RDD, or PHD in OB and bMSC of OVX rats. No significant changes in either OPG or RANKL expression were observed in OB and bMSC of OVX rats treated with CSD.

Conclusions: Our study showed that RDD and PHD increased bone formation by stimulating overexpression of OPG and downregulation of RANKL in OB and bMSC. This suggests that RDD and PHD may be used as alternative therapeutic agents for postmenopausal osteoporosis.

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Effects of RDD, PHD, and CSD on bone formation. Trabecular bone formation was visualized by using methylene blue staining. See the details in the Methods section of the text.
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Figure 1: Effects of RDD, PHD, and CSD on bone formation. Trabecular bone formation was visualized by using methylene blue staining. See the details in the Methods section of the text.

Mentions: To investigate the effects of the herbal medicines on overall bone formation, we analyzed trabecular bone formation in the undecalcified tibial sections from tibiae of OVX rats treated with RDD, PHD, and CSD using methylene blue staining (Figure 1). Trabecular bone formation was estimated by numbers of stained trabeculae in the undecalcified section. Figure 1 shows that trabecular bone formation was reduced significantly in the ovariectomized rats compared to rats in normal control and sham groups, while this effect was remarkably reversed by E2 treatment. Numbers of trabeculae in the stained tibial section were significantly increased in OVX rats treated with RSD and PHD, but not with CSD (Figure 1), compared to that in OVX control.


Therapeutic effects of radix dipsaci, pyrola herb, and Cynomorium songaricum on bone metabolism of ovariectomized rats.

Liu M, Xiao GG, Rong P, Zhang Z, Dong J, Zhao H, Li H, Li Y, Pan J, Liu H, Wang W, Zha Q, Ju D - BMC Complement Altern Med (2012)

Effects of RDD, PHD, and CSD on bone formation. Trabecular bone formation was visualized by using methylene blue staining. See the details in the Methods section of the text.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585854&req=5

Figure 1: Effects of RDD, PHD, and CSD on bone formation. Trabecular bone formation was visualized by using methylene blue staining. See the details in the Methods section of the text.
Mentions: To investigate the effects of the herbal medicines on overall bone formation, we analyzed trabecular bone formation in the undecalcified tibial sections from tibiae of OVX rats treated with RDD, PHD, and CSD using methylene blue staining (Figure 1). Trabecular bone formation was estimated by numbers of stained trabeculae in the undecalcified section. Figure 1 shows that trabecular bone formation was reduced significantly in the ovariectomized rats compared to rats in normal control and sham groups, while this effect was remarkably reversed by E2 treatment. Numbers of trabeculae in the stained tibial section were significantly increased in OVX rats treated with RSD and PHD, but not with CSD (Figure 1), compared to that in OVX control.

Bottom Line: Bone mass and bone turnover were analyzed by histomorphometry.Treatment with either RDD or PHD enhanced trabecular bone formation remarkably.Our study showed that RDD and PHD increased bone formation by stimulating overexpression of OPG and downregulation of RANKL in OB and bMSC.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Basic Theory, China Academy of Chinese Medical Sciences, South Small Street 16, Dongzhimennei, Dongcheng District, Beijing 100700, Peoples Republic of ChineChina.

ABSTRACT

Background: The objective of this study was to evaluate the effects of herbal medicines, such as Radix Dipsaci (RDD), Pyrola Herb (PHD), and Cynomorium songaricum decoction (CSD), on osteoporotic rats induced by ovariectomy (OVX).

Methods: OVX or sham operations were performed on 69 virgin Wistar rats that were divided into six groups: sham (sham, n = 12), OVX control group (OVX, n = 12), and OVX rats with treatments (diethylstilbestrol, E2, n = 12; RDD, n = 11, PHD, n = 11, and CSD, n = 11). Non-surgical rats served as normal control (NC, n = 12). The treatments began four weeks after surgery and lasted for 12 weeks. Bone mass and bone turnover were analyzed by histomorphometry. Levels of protein expression and mRNA of OPG and RANKL in osteoblasts (OB) and bone marrow stromal cells (bMSC) were evaluated by immunohistochemistry and in situ hybridization.

Results: Compared to NC and sham rats, trabecular bone formation was significantly reduced in OVX rats, but restored in E2-treated rats. Treatment with either RDD or PHD enhanced trabecular bone formation remarkably. No significant change of bone formation was observed in CSD-treated rats. OPG expression of protein and mRNA was reduced significantly in OB and bMSC of OVX control rats. RANKL expression of protein and mRNA was increased significantly in OB and bMSC of OVX control rats. These effects were substantially reversed (increased in OPG and decreased in RANKL) by treatment with E2, RDD, or PHD in OB and bMSC of OVX rats. No significant changes in either OPG or RANKL expression were observed in OB and bMSC of OVX rats treated with CSD.

Conclusions: Our study showed that RDD and PHD increased bone formation by stimulating overexpression of OPG and downregulation of RANKL in OB and bMSC. This suggests that RDD and PHD may be used as alternative therapeutic agents for postmenopausal osteoporosis.

Show MeSH
Related in: MedlinePlus