Limits...
Vasorelaxant effect of Prunus yedoensis bark.

Lee K, Ham I, Yang G, Lee M, Bu Y, Kim H, Choi HY - BMC Complement Altern Med (2013)

Bottom Line: However, the vasorelaxant effects of MEPY on endothelium-denuded aortic rings were lower than endothelium-intact aortic rings.The vasorelaxant effects of MEPY on endothelium-intact aortic rings were reduced by pre-treatment with L-NAME, methylene blue, or ODQ.Our results suggest that MEPY exerts its vasorelaxant effects via the activation of NO formation by means of L-Arg and NO-cGMP pathways and via the blockage of extracellular Ca(2+) channels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701 Seoul, Republic of Korea.

ABSTRACT

Background: Prunus yedoensis Matsum. is used as traditional medicine-'Yaeng-Pi' or 'Hua-Pi'-in Japan and Korea. However, no studies have examined the pharmacological activities of the P. yedoensis bark. Only the antioxidant and antiviral activities of P. yedoensis fruit and the anti-hyperglycaemic effect of P. yedoensis leaf have been investigated. While studying the antihypertensive effects of several medicinal plants, we found that a methanol extract of P. yedoensis bark (MEPY) had distinct vasorelaxant effects on rat aortic rings.

Methods: The aortic rings were removed from Sprague-Dawley rats and suspended in organ chambers containing 10 ml Krebs-Henseleit solution. The aortic rings were placed between 2 tungsten stirrups and connected to an isometric force transducer. Changes in tension were recorded via isometric transducers connected to a data acquisition system.

Results: MEPY relaxed the contraction induced by phenylephrine (PE) both in endothelium-intact and endothelium-denuded aortic rings concentration dependently. However, the vasorelaxant effects of MEPY on endothelium-denuded aortic rings were lower than endothelium-intact aortic rings. The vasorelaxant effects of MEPY on endothelium-intact aortic rings were reduced by pre-treatment with L-NAME, methylene blue, or ODQ. However, pre-treatment with indomethacin, atropine, glibenclamide, tetraethylammonium, or 4-aminopyridine had no affection. In addition, MEPY inhibited the contraction induced by extracellular Ca(2+) in endothelium-denuded rat thoracic aorta rings pre-contracted by PE (1 μM) or KCl (60 mM) in Ca(2+)-free solution.

Conclusions: Our results suggest that MEPY exerts its vasorelaxant effects via the activation of NO formation by means of L-Arg and NO-cGMP pathways and via the blockage of extracellular Ca(2+) channels.

Show MeSH

Related in: MedlinePlus

Concentration-dependent relaxant effects of MEPY on phenylephrine (PE, 1 μM)-pre-contracted rat aortic rings with [(E+)] or without [(E-)] endothelium (A) in Krebs-Henseleit solution. Control groups were not treated with MEPY. The MEPY induced-relaxant traces of aortic rings with [(E+)] (B) or without [(E-)] endothelium (C). The relaxant effects of MEPY on isolated rat aortic rings were calculated as a percentage of the contraction in response to PE. Values are expressed as mean ± SEM (n = 8). *P < 0.05, **P < 0.01 vs. MEPY E (−).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3585796&req=5

Figure 1: Concentration-dependent relaxant effects of MEPY on phenylephrine (PE, 1 μM)-pre-contracted rat aortic rings with [(E+)] or without [(E-)] endothelium (A) in Krebs-Henseleit solution. Control groups were not treated with MEPY. The MEPY induced-relaxant traces of aortic rings with [(E+)] (B) or without [(E-)] endothelium (C). The relaxant effects of MEPY on isolated rat aortic rings were calculated as a percentage of the contraction in response to PE. Values are expressed as mean ± SEM (n = 8). *P < 0.05, **P < 0.01 vs. MEPY E (−).

Mentions: MEPY caused concentration-dependent relaxation in both endothelium-intact and endothelium-denuded aortic rings pre-contracted by PE (1 μM) treatment. However, endothelium-intact aortic rings were more relaxed than endothelium-denuded aortic rings. The maximal relaxant effect was 94.0% ± 2.7% and 49.4% ± 0.7% for endothelium-intact and endothelium-denuded aortic rings, respectively (Figure1).


Vasorelaxant effect of Prunus yedoensis bark.

Lee K, Ham I, Yang G, Lee M, Bu Y, Kim H, Choi HY - BMC Complement Altern Med (2013)

Concentration-dependent relaxant effects of MEPY on phenylephrine (PE, 1 μM)-pre-contracted rat aortic rings with [(E+)] or without [(E-)] endothelium (A) in Krebs-Henseleit solution. Control groups were not treated with MEPY. The MEPY induced-relaxant traces of aortic rings with [(E+)] (B) or without [(E-)] endothelium (C). The relaxant effects of MEPY on isolated rat aortic rings were calculated as a percentage of the contraction in response to PE. Values are expressed as mean ± SEM (n = 8). *P < 0.05, **P < 0.01 vs. MEPY E (−).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585796&req=5

Figure 1: Concentration-dependent relaxant effects of MEPY on phenylephrine (PE, 1 μM)-pre-contracted rat aortic rings with [(E+)] or without [(E-)] endothelium (A) in Krebs-Henseleit solution. Control groups were not treated with MEPY. The MEPY induced-relaxant traces of aortic rings with [(E+)] (B) or without [(E-)] endothelium (C). The relaxant effects of MEPY on isolated rat aortic rings were calculated as a percentage of the contraction in response to PE. Values are expressed as mean ± SEM (n = 8). *P < 0.05, **P < 0.01 vs. MEPY E (−).
Mentions: MEPY caused concentration-dependent relaxation in both endothelium-intact and endothelium-denuded aortic rings pre-contracted by PE (1 μM) treatment. However, endothelium-intact aortic rings were more relaxed than endothelium-denuded aortic rings. The maximal relaxant effect was 94.0% ± 2.7% and 49.4% ± 0.7% for endothelium-intact and endothelium-denuded aortic rings, respectively (Figure1).

Bottom Line: However, the vasorelaxant effects of MEPY on endothelium-denuded aortic rings were lower than endothelium-intact aortic rings.The vasorelaxant effects of MEPY on endothelium-intact aortic rings were reduced by pre-treatment with L-NAME, methylene blue, or ODQ.Our results suggest that MEPY exerts its vasorelaxant effects via the activation of NO formation by means of L-Arg and NO-cGMP pathways and via the blockage of extracellular Ca(2+) channels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Herbology, College of Korean Medicine, Kyung Hee University, 1 Hoegi-Dong, Dongdaemun-Gu, 130-701 Seoul, Republic of Korea.

ABSTRACT

Background: Prunus yedoensis Matsum. is used as traditional medicine-'Yaeng-Pi' or 'Hua-Pi'-in Japan and Korea. However, no studies have examined the pharmacological activities of the P. yedoensis bark. Only the antioxidant and antiviral activities of P. yedoensis fruit and the anti-hyperglycaemic effect of P. yedoensis leaf have been investigated. While studying the antihypertensive effects of several medicinal plants, we found that a methanol extract of P. yedoensis bark (MEPY) had distinct vasorelaxant effects on rat aortic rings.

Methods: The aortic rings were removed from Sprague-Dawley rats and suspended in organ chambers containing 10 ml Krebs-Henseleit solution. The aortic rings were placed between 2 tungsten stirrups and connected to an isometric force transducer. Changes in tension were recorded via isometric transducers connected to a data acquisition system.

Results: MEPY relaxed the contraction induced by phenylephrine (PE) both in endothelium-intact and endothelium-denuded aortic rings concentration dependently. However, the vasorelaxant effects of MEPY on endothelium-denuded aortic rings were lower than endothelium-intact aortic rings. The vasorelaxant effects of MEPY on endothelium-intact aortic rings were reduced by pre-treatment with L-NAME, methylene blue, or ODQ. However, pre-treatment with indomethacin, atropine, glibenclamide, tetraethylammonium, or 4-aminopyridine had no affection. In addition, MEPY inhibited the contraction induced by extracellular Ca(2+) in endothelium-denuded rat thoracic aorta rings pre-contracted by PE (1 μM) or KCl (60 mM) in Ca(2+)-free solution.

Conclusions: Our results suggest that MEPY exerts its vasorelaxant effects via the activation of NO formation by means of L-Arg and NO-cGMP pathways and via the blockage of extracellular Ca(2+) channels.

Show MeSH
Related in: MedlinePlus