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MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells.

Wang C, Zheng X, Shen C, Shi Y - J. Exp. Clin. Cancer Res. (2012)

Bottom Line: Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also.Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.These data suggest that miR-203 may function as a tumor suppressor in TNBC cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Breast Oncology, Tianjin Medical University Cancer Hospital, Huanhuxi Ave, Tianjin 300060, China.

ABSTRACT

Background: This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC).

Methods: Real-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets.

Results: Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.

Conclusions: These data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.

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Related in: MedlinePlus

Over-expression of LASP1 significantly attenuated the effect of miR-203 on the inhibition of MDA-MB-231 cell migration. (A) LASP1 protein expression was detected by western blot and normalized to the levels of β-actin protein. (B) Transwell migration assay was performed to detect the migratory capacity of MDA-MB-231 cells. *, P < 0.05.
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Figure 7: Over-expression of LASP1 significantly attenuated the effect of miR-203 on the inhibition of MDA-MB-231 cell migration. (A) LASP1 protein expression was detected by western blot and normalized to the levels of β-actin protein. (B) Transwell migration assay was performed to detect the migratory capacity of MDA-MB-231 cells. *, P < 0.05.

Mentions: To provide direct evidence that miR-203 inhibits the migration of TNBC cells through the LASP1-mediated signal pathway, we transfected MDA-MB-231 cells with miR-203 precursor and pcDNA-LASP1. We confirmed the effect of the transfection by western blot (Figure 7A). The migration assay showed that the over-expression of LASP1 could partially rescue the migratory capacity of MDA-MB-231 cells treated with the miR-203 precursor (Figure 7B).


MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells.

Wang C, Zheng X, Shen C, Shi Y - J. Exp. Clin. Cancer Res. (2012)

Over-expression of LASP1 significantly attenuated the effect of miR-203 on the inhibition of MDA-MB-231 cell migration. (A) LASP1 protein expression was detected by western blot and normalized to the levels of β-actin protein. (B) Transwell migration assay was performed to detect the migratory capacity of MDA-MB-231 cells. *, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585778&req=5

Figure 7: Over-expression of LASP1 significantly attenuated the effect of miR-203 on the inhibition of MDA-MB-231 cell migration. (A) LASP1 protein expression was detected by western blot and normalized to the levels of β-actin protein. (B) Transwell migration assay was performed to detect the migratory capacity of MDA-MB-231 cells. *, P < 0.05.
Mentions: To provide direct evidence that miR-203 inhibits the migration of TNBC cells through the LASP1-mediated signal pathway, we transfected MDA-MB-231 cells with miR-203 precursor and pcDNA-LASP1. We confirmed the effect of the transfection by western blot (Figure 7A). The migration assay showed that the over-expression of LASP1 could partially rescue the migratory capacity of MDA-MB-231 cells treated with the miR-203 precursor (Figure 7B).

Bottom Line: Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also.Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.These data suggest that miR-203 may function as a tumor suppressor in TNBC cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Breast Oncology, Tianjin Medical University Cancer Hospital, Huanhuxi Ave, Tianjin 300060, China.

ABSTRACT

Background: This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC).

Methods: Real-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets.

Results: Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.

Conclusions: These data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.

Show MeSH
Related in: MedlinePlus