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MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells.

Wang C, Zheng X, Shen C, Shi Y - J. Exp. Clin. Cancer Res. (2012)

Bottom Line: Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also.Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.These data suggest that miR-203 may function as a tumor suppressor in TNBC cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Breast Oncology, Tianjin Medical University Cancer Hospital, Huanhuxi Ave, Tianjin 300060, China.

ABSTRACT

Background: This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC).

Methods: Real-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets.

Results: Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.

Conclusions: These data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.

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Related in: MedlinePlus

Repressing BIRC5 expression could inhibit the proliferation of MDA-MB-231 cells. (A) Immunoblots of BIRC5 protein in MDA-MB-231 cells treated with control siRNA or BIRC5 siRNA. β-actin was used as a loading control. (B) Colony formation assay was used to measure cell proliferative capacity in MDA-MB-231 cells treated with control siRNA or BIRC5 siRNA. *, P < 0.05.
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Figure 4: Repressing BIRC5 expression could inhibit the proliferation of MDA-MB-231 cells. (A) Immunoblots of BIRC5 protein in MDA-MB-231 cells treated with control siRNA or BIRC5 siRNA. β-actin was used as a loading control. (B) Colony formation assay was used to measure cell proliferative capacity in MDA-MB-231 cells treated with control siRNA or BIRC5 siRNA. *, P < 0.05.

Mentions: To investigate the effect of BIRC5 on the proliferation of TNBC cell, we employed MDA-MB-231 cells as the model system to perform the subsequent studies. We evaluated the cell proliferative capacity of MDA-MB-231 cells transfected with BIRC5 siRNA (or control siRNA). The expression of BIRC5 protein in the cells transfected with BIRC5 siRNA was significantly decreased in comparison with that of cells transfected with control siRNA (Figure 4A), indicating that the expression of BIRC5 was effectively inhibited by BIRC5 siRNA. Subsequent studies showed that the proliferative capacity of cells transfected with BIRC5 siRNA was significantly lower than that of cells treated with control siRNA (Figure 4B).


MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells.

Wang C, Zheng X, Shen C, Shi Y - J. Exp. Clin. Cancer Res. (2012)

Repressing BIRC5 expression could inhibit the proliferation of MDA-MB-231 cells. (A) Immunoblots of BIRC5 protein in MDA-MB-231 cells treated with control siRNA or BIRC5 siRNA. β-actin was used as a loading control. (B) Colony formation assay was used to measure cell proliferative capacity in MDA-MB-231 cells treated with control siRNA or BIRC5 siRNA. *, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585778&req=5

Figure 4: Repressing BIRC5 expression could inhibit the proliferation of MDA-MB-231 cells. (A) Immunoblots of BIRC5 protein in MDA-MB-231 cells treated with control siRNA or BIRC5 siRNA. β-actin was used as a loading control. (B) Colony formation assay was used to measure cell proliferative capacity in MDA-MB-231 cells treated with control siRNA or BIRC5 siRNA. *, P < 0.05.
Mentions: To investigate the effect of BIRC5 on the proliferation of TNBC cell, we employed MDA-MB-231 cells as the model system to perform the subsequent studies. We evaluated the cell proliferative capacity of MDA-MB-231 cells transfected with BIRC5 siRNA (or control siRNA). The expression of BIRC5 protein in the cells transfected with BIRC5 siRNA was significantly decreased in comparison with that of cells transfected with control siRNA (Figure 4A), indicating that the expression of BIRC5 was effectively inhibited by BIRC5 siRNA. Subsequent studies showed that the proliferative capacity of cells transfected with BIRC5 siRNA was significantly lower than that of cells treated with control siRNA (Figure 4B).

Bottom Line: Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also.Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.These data suggest that miR-203 may function as a tumor suppressor in TNBC cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Breast Oncology, Tianjin Medical University Cancer Hospital, Huanhuxi Ave, Tianjin 300060, China.

ABSTRACT

Background: This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC).

Methods: Real-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets.

Results: Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.

Conclusions: These data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.

Show MeSH
Related in: MedlinePlus