Limits...
MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells.

Wang C, Zheng X, Shen C, Shi Y - J. Exp. Clin. Cancer Res. (2012)

Bottom Line: Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also.Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.These data suggest that miR-203 may function as a tumor suppressor in TNBC cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Breast Oncology, Tianjin Medical University Cancer Hospital, Huanhuxi Ave, Tianjin 300060, China.

ABSTRACT

Background: This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC).

Methods: Real-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets.

Results: Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.

Conclusions: These data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.

Show MeSH

Related in: MedlinePlus

miR-203 inhibited proliferation and migration of TNBC cells. (A) The colony formation assay was used to measure cell proliferation capacity in MDA-MB-468 and MDA-MB-231 cells treated with control miRNA or miR-203 precursor. (B) A transwell migration assay was performed to detect the migratory capacity of MDA-MB-468 and MDA-MB-231 cells. *, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3585778&req=5

Figure 2: miR-203 inhibited proliferation and migration of TNBC cells. (A) The colony formation assay was used to measure cell proliferation capacity in MDA-MB-468 and MDA-MB-231 cells treated with control miRNA or miR-203 precursor. (B) A transwell migration assay was performed to detect the migratory capacity of MDA-MB-468 and MDA-MB-231 cells. *, P < 0.05.

Mentions: Previous reports have shown that the over-expression of miR-203 has an impact on growth in prostate and laryngeal cancer cell lines [13,14]. Therefore, we investigated the effect of miR-203 on the proliferation of TNBC cells. Colony formation assay showed that a statistically significant inhibition of TNBC cell proliferation occurred after treatment with the miR-203 precursor (Figure 2A). To investigate whether miR-203 inhibits the migration of TNBC cells, we performed a transwell migration assay. Interestingly, the over-expression of miR-203 repressed the migration of the MDA-MB-231 and MDA-MB-468 cells. Cell mobility was significantly decreased by approximately 50% in miR-203-transfected cells compared with the control miRNA-transfected cells (Figure 2B). These observations suggest that miR-203 over-expression suppresses the mobility of TNBC cells in vitro.


MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells.

Wang C, Zheng X, Shen C, Shi Y - J. Exp. Clin. Cancer Res. (2012)

miR-203 inhibited proliferation and migration of TNBC cells. (A) The colony formation assay was used to measure cell proliferation capacity in MDA-MB-468 and MDA-MB-231 cells treated with control miRNA or miR-203 precursor. (B) A transwell migration assay was performed to detect the migratory capacity of MDA-MB-468 and MDA-MB-231 cells. *, P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585778&req=5

Figure 2: miR-203 inhibited proliferation and migration of TNBC cells. (A) The colony formation assay was used to measure cell proliferation capacity in MDA-MB-468 and MDA-MB-231 cells treated with control miRNA or miR-203 precursor. (B) A transwell migration assay was performed to detect the migratory capacity of MDA-MB-468 and MDA-MB-231 cells. *, P < 0.05.
Mentions: Previous reports have shown that the over-expression of miR-203 has an impact on growth in prostate and laryngeal cancer cell lines [13,14]. Therefore, we investigated the effect of miR-203 on the proliferation of TNBC cells. Colony formation assay showed that a statistically significant inhibition of TNBC cell proliferation occurred after treatment with the miR-203 precursor (Figure 2A). To investigate whether miR-203 inhibits the migration of TNBC cells, we performed a transwell migration assay. Interestingly, the over-expression of miR-203 repressed the migration of the MDA-MB-231 and MDA-MB-468 cells. Cell mobility was significantly decreased by approximately 50% in miR-203-transfected cells compared with the control miRNA-transfected cells (Figure 2B). These observations suggest that miR-203 over-expression suppresses the mobility of TNBC cells in vitro.

Bottom Line: Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also.Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.These data suggest that miR-203 may function as a tumor suppressor in TNBC cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Breast Oncology, Tianjin Medical University Cancer Hospital, Huanhuxi Ave, Tianjin 300060, China.

ABSTRACT

Background: This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC).

Methods: Real-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets.

Results: Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.

Conclusions: These data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.

Show MeSH
Related in: MedlinePlus