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Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivo.

Hagena H, Manahan-Vaughan D - Front Integr Neurosci (2013)

Bottom Line: In contrast, at AC-CA3 synapses, translation inhibitors prevented intermediate/late-LTP and late-LTD only.Transcription effects were also synapse-specific: whereas transcription inhibitors inhibited late-LTP and late-LTD (>3 h) at mf-CA3 synapses, at AC-CA3 synapses, protein transcription affected early-LTP and late-LTD.These results show that the AC-CA3 and mf-CA3 synapses display different properties in terms of their protein synthesis dependency, suggesting different roles in the processing of short- and long term synaptic plasticity.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurophysiology, Medical Faculty, Ruhr University Bochum Bochum, Germany ; International Graduate School for Neuroscience, Ruhr University Bochum Bochum, Germany.

ABSTRACT
Long-term potentiation (LTP) and long-term depression (LTD) are two mechanisms involved in the long-term storage of information in hippocampal synapses. In the hippocampal CA1 region, the late phases of LTP and LTD are protein-synthesis dependent. In the dentate gyrus, late-LTP but not LTD requires protein synthesis. The protein synthesis-dependency of persistent plasticity at CA3 synapses has not yet been characterized. Here, the roles of protein transcription and translation at mossy fiber (mf) and associational/commissural (AC)- synapses were studied in freely behaving rats. In control animals, low-frequency stimulation (LFS) evoked robust LTD (>24 h), whereas high-frequency stimulation (HFS) elicited robust LTP (>24 h) at both mf-CA3 and AC-CA3 synapses. Translation inhibitors prevented early and late phases of LTP and LTD at mf-CA3 synapses. In contrast, at AC-CA3 synapses, translation inhibitors prevented intermediate/late-LTP and late-LTD only. Transcription effects were also synapse-specific: whereas transcription inhibitors inhibited late-LTP and late-LTD (>3 h) at mf-CA3 synapses, at AC-CA3 synapses, protein transcription affected early-LTP and late-LTD. These results show that the AC-CA3 and mf-CA3 synapses display different properties in terms of their protein synthesis dependency, suggesting different roles in the processing of short- and long term synaptic plasticity.

No MeSH data available.


Related in: MedlinePlus

Application of translational inhibitors affect the early and late phase of long-term synaptic plasticity at mossy fiber–CA3 synapses. (A) High-frequency stimulation (HFS, 4 trains, 100 Hz) induces LTP that lasts for over 24 h in vehicle-injected animals. Application of the protein-synthesis inhibitor anisomycin (4.8 μg) inhibits the early and late phase of LTP. (B) Application of LFS (1 Hz, 900 pulses) induces LTD (>24 h) in vehicle-injected animals. Injection of anisomycin inhibits early- and late LTP. (C) Application of HFS elicits LTP (>24 h) in vehicle-injected animals. Injection of emetine (240 μg) inhibits the early and late phase of LTP. (D) Injection of emetine leads to an impairment of both the early and late phase of LTD compared to vehicle-injected controls. Line breaks indicate change of time scale. (E) Traces in the left panel represent responses recorded during an LTP control experiment (upper traces) and during an experiment where anisomycin was applied prior to HFS (lower traces): analog examples representing pre-HFS (i), 5 min post-HFS (ii) and 24 h post-HFS (iii) are shown. Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. Analog traces in the right panel are recorded during an experiment in which only LFS was applied (upper traces) and during an anisomycin experiment (lower traces) pre-LFS (i), 5 min post-LFS (ii) and 24 h post-LFS (iii). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. (F) Analogs in the left panel depict fEPSPs recorded during a control experiment (upper traces) and during an emetine experiment (lower traces) pre-HFS (i), 5 min post-HFS (ii), and 24 h post-HFS (iii). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. Analog traces in the right panel represent recordings taken (i) pre-LFS, (ii) 5 min post-LFS, and (iii) 24 h post-LFS in the presence of vehicle (upper traces) or emetine (lower traces). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms.
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Figure 2: Application of translational inhibitors affect the early and late phase of long-term synaptic plasticity at mossy fiber–CA3 synapses. (A) High-frequency stimulation (HFS, 4 trains, 100 Hz) induces LTP that lasts for over 24 h in vehicle-injected animals. Application of the protein-synthesis inhibitor anisomycin (4.8 μg) inhibits the early and late phase of LTP. (B) Application of LFS (1 Hz, 900 pulses) induces LTD (>24 h) in vehicle-injected animals. Injection of anisomycin inhibits early- and late LTP. (C) Application of HFS elicits LTP (>24 h) in vehicle-injected animals. Injection of emetine (240 μg) inhibits the early and late phase of LTP. (D) Injection of emetine leads to an impairment of both the early and late phase of LTD compared to vehicle-injected controls. Line breaks indicate change of time scale. (E) Traces in the left panel represent responses recorded during an LTP control experiment (upper traces) and during an experiment where anisomycin was applied prior to HFS (lower traces): analog examples representing pre-HFS (i), 5 min post-HFS (ii) and 24 h post-HFS (iii) are shown. Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. Analog traces in the right panel are recorded during an experiment in which only LFS was applied (upper traces) and during an anisomycin experiment (lower traces) pre-LFS (i), 5 min post-LFS (ii) and 24 h post-LFS (iii). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. (F) Analogs in the left panel depict fEPSPs recorded during a control experiment (upper traces) and during an emetine experiment (lower traces) pre-HFS (i), 5 min post-HFS (ii), and 24 h post-HFS (iii). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. Analog traces in the right panel represent recordings taken (i) pre-LFS, (ii) 5 min post-LFS, and (iii) 24 h post-LFS in the presence of vehicle (upper traces) or emetine (lower traces). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms.

Mentions: Robust LTP (>24 h) in vehicle-treated animals was induced with HFS comprising 4 pulses of 100 Hz (Figures 2A,E). Prior treatment with anisomycin resulted in a significant inhibition of the early and late phases of LTP compared to vehicle-treated animals (ANOVA, F(1, 7) = 6.04; p = 0.04; interaction effect: F(26, 182) = 1.558, p = 0.049; n = 5, Figures 2A,E).


Differentiation in the protein synthesis-dependency of persistent synaptic plasticity in mossy fiber and associational/commissural CA3 synapses in vivo.

Hagena H, Manahan-Vaughan D - Front Integr Neurosci (2013)

Application of translational inhibitors affect the early and late phase of long-term synaptic plasticity at mossy fiber–CA3 synapses. (A) High-frequency stimulation (HFS, 4 trains, 100 Hz) induces LTP that lasts for over 24 h in vehicle-injected animals. Application of the protein-synthesis inhibitor anisomycin (4.8 μg) inhibits the early and late phase of LTP. (B) Application of LFS (1 Hz, 900 pulses) induces LTD (>24 h) in vehicle-injected animals. Injection of anisomycin inhibits early- and late LTP. (C) Application of HFS elicits LTP (>24 h) in vehicle-injected animals. Injection of emetine (240 μg) inhibits the early and late phase of LTP. (D) Injection of emetine leads to an impairment of both the early and late phase of LTD compared to vehicle-injected controls. Line breaks indicate change of time scale. (E) Traces in the left panel represent responses recorded during an LTP control experiment (upper traces) and during an experiment where anisomycin was applied prior to HFS (lower traces): analog examples representing pre-HFS (i), 5 min post-HFS (ii) and 24 h post-HFS (iii) are shown. Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. Analog traces in the right panel are recorded during an experiment in which only LFS was applied (upper traces) and during an anisomycin experiment (lower traces) pre-LFS (i), 5 min post-LFS (ii) and 24 h post-LFS (iii). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. (F) Analogs in the left panel depict fEPSPs recorded during a control experiment (upper traces) and during an emetine experiment (lower traces) pre-HFS (i), 5 min post-HFS (ii), and 24 h post-HFS (iii). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. Analog traces in the right panel represent recordings taken (i) pre-LFS, (ii) 5 min post-LFS, and (iii) 24 h post-LFS in the presence of vehicle (upper traces) or emetine (lower traces). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585440&req=5

Figure 2: Application of translational inhibitors affect the early and late phase of long-term synaptic plasticity at mossy fiber–CA3 synapses. (A) High-frequency stimulation (HFS, 4 trains, 100 Hz) induces LTP that lasts for over 24 h in vehicle-injected animals. Application of the protein-synthesis inhibitor anisomycin (4.8 μg) inhibits the early and late phase of LTP. (B) Application of LFS (1 Hz, 900 pulses) induces LTD (>24 h) in vehicle-injected animals. Injection of anisomycin inhibits early- and late LTP. (C) Application of HFS elicits LTP (>24 h) in vehicle-injected animals. Injection of emetine (240 μg) inhibits the early and late phase of LTP. (D) Injection of emetine leads to an impairment of both the early and late phase of LTD compared to vehicle-injected controls. Line breaks indicate change of time scale. (E) Traces in the left panel represent responses recorded during an LTP control experiment (upper traces) and during an experiment where anisomycin was applied prior to HFS (lower traces): analog examples representing pre-HFS (i), 5 min post-HFS (ii) and 24 h post-HFS (iii) are shown. Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. Analog traces in the right panel are recorded during an experiment in which only LFS was applied (upper traces) and during an anisomycin experiment (lower traces) pre-LFS (i), 5 min post-LFS (ii) and 24 h post-LFS (iii). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. (F) Analogs in the left panel depict fEPSPs recorded during a control experiment (upper traces) and during an emetine experiment (lower traces) pre-HFS (i), 5 min post-HFS (ii), and 24 h post-HFS (iii). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms. Analog traces in the right panel represent recordings taken (i) pre-LFS, (ii) 5 min post-LFS, and (iii) 24 h post-LFS in the presence of vehicle (upper traces) or emetine (lower traces). Vertical scale bar: 2 mV, horizontal scale bar: 8 ms.
Mentions: Robust LTP (>24 h) in vehicle-treated animals was induced with HFS comprising 4 pulses of 100 Hz (Figures 2A,E). Prior treatment with anisomycin resulted in a significant inhibition of the early and late phases of LTP compared to vehicle-treated animals (ANOVA, F(1, 7) = 6.04; p = 0.04; interaction effect: F(26, 182) = 1.558, p = 0.049; n = 5, Figures 2A,E).

Bottom Line: In contrast, at AC-CA3 synapses, translation inhibitors prevented intermediate/late-LTP and late-LTD only.Transcription effects were also synapse-specific: whereas transcription inhibitors inhibited late-LTP and late-LTD (>3 h) at mf-CA3 synapses, at AC-CA3 synapses, protein transcription affected early-LTP and late-LTD.These results show that the AC-CA3 and mf-CA3 synapses display different properties in terms of their protein synthesis dependency, suggesting different roles in the processing of short- and long term synaptic plasticity.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurophysiology, Medical Faculty, Ruhr University Bochum Bochum, Germany ; International Graduate School for Neuroscience, Ruhr University Bochum Bochum, Germany.

ABSTRACT
Long-term potentiation (LTP) and long-term depression (LTD) are two mechanisms involved in the long-term storage of information in hippocampal synapses. In the hippocampal CA1 region, the late phases of LTP and LTD are protein-synthesis dependent. In the dentate gyrus, late-LTP but not LTD requires protein synthesis. The protein synthesis-dependency of persistent plasticity at CA3 synapses has not yet been characterized. Here, the roles of protein transcription and translation at mossy fiber (mf) and associational/commissural (AC)- synapses were studied in freely behaving rats. In control animals, low-frequency stimulation (LFS) evoked robust LTD (>24 h), whereas high-frequency stimulation (HFS) elicited robust LTP (>24 h) at both mf-CA3 and AC-CA3 synapses. Translation inhibitors prevented early and late phases of LTP and LTD at mf-CA3 synapses. In contrast, at AC-CA3 synapses, translation inhibitors prevented intermediate/late-LTP and late-LTD only. Transcription effects were also synapse-specific: whereas transcription inhibitors inhibited late-LTP and late-LTD (>3 h) at mf-CA3 synapses, at AC-CA3 synapses, protein transcription affected early-LTP and late-LTD. These results show that the AC-CA3 and mf-CA3 synapses display different properties in terms of their protein synthesis dependency, suggesting different roles in the processing of short- and long term synaptic plasticity.

No MeSH data available.


Related in: MedlinePlus