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Tracking dynamic microvascular changes during healing after complete biopsy punch on the mouse pinna using optical microangiography.

Jung Y, Dziennis S, Zhi Z, Reif R, Zheng Y, Wang RK - PLoS ONE (2013)

Bottom Line: The highest rate of wound closure occurred between days 8 and 22.The vessel tortuosity increased during this time suggesting angiogenesis.The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.

ABSTRACT
Optical microangiography (OMAG) and Doppler optical microangiography (DOMAG) are two non-invasive techniques capable of determining the tissue microstructural content, microvasculature angiography, and blood flow velocity and direction. These techniques were used to visualize the acute and chronic microvascular and tissue responses upon an injury in vivo. A tissue wound was induced using a 0.5 mm biopsy punch on a mouse pinna. The changes in the microangiography, blood flow velocity and direction were quantified for the acute (<30 min) wound response and the changes in the tissue structure and microangiography were determined for the chronic wound response (30 min-60 days). The initial wound triggered recruitment of peripheral capillaries, as well as redirection of main arterial and venous blood flow within 3 min. The complex vascular networks and new vessel formation were quantified during the chronic response using fractal dimension. The highest rate of wound closure occurred between days 8 and 22. The vessel tortuosity increased during this time suggesting angiogenesis. Taken together, these data signify that OMAG has the capability to track acute and chronic changes in blood flow, microangiography and structure during wound healing. The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.

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OMAG cross-sectional structural images of the mouse pinna in vivo at several time points.(A) The image size is 1.2 mm×1.2 mm. A, auricular cartilage, E, Epidermis. The epidermal layer increased in thickness and migrated together to from a closure, but the auricular cartilage never fully recovered at 60 days post-injury. (B) Quantification of relative change in epidermal thickness over time.
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pone-0057976-g004: OMAG cross-sectional structural images of the mouse pinna in vivo at several time points.(A) The image size is 1.2 mm×1.2 mm. A, auricular cartilage, E, Epidermis. The epidermal layer increased in thickness and migrated together to from a closure, but the auricular cartilage never fully recovered at 60 days post-injury. (B) Quantification of relative change in epidermal thickness over time.

Mentions: Structure images of a cross-section of the pinna obtained with the OCT system is shown in Figure 4. The location of the epidermis (E) and auricular cartilage (A) is indicated by corresponding arrows. By day 4 the edges of the wound are raised compared with the control image. The epidermal thickness (Fig.4B) in the structural images drastically increases at day 4 and gradually subsides thereafter, although never returning to the pre-injury thickness within the timeframe investigated (60 days in this study). To determine the behavior of blood vessel development and structural wound closure during wound healing, a series of OMAG images (Figs. 5 and 6) were obtained on the same animal before and at 30 min and 4, 8, 12, 18, 22, 26, 32, 46 and 60 days after the biopsy punch was performed on the mouse pinna. The branches of an adjacent artery and vein around the wound became gradually thicker starting from day 4 up to day 26 (blue arrow in Figure 5) suggesting a hyperemic response to the tissue surrounding ischemic area.


Tracking dynamic microvascular changes during healing after complete biopsy punch on the mouse pinna using optical microangiography.

Jung Y, Dziennis S, Zhi Z, Reif R, Zheng Y, Wang RK - PLoS ONE (2013)

OMAG cross-sectional structural images of the mouse pinna in vivo at several time points.(A) The image size is 1.2 mm×1.2 mm. A, auricular cartilage, E, Epidermis. The epidermal layer increased in thickness and migrated together to from a closure, but the auricular cartilage never fully recovered at 60 days post-injury. (B) Quantification of relative change in epidermal thickness over time.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3585416&req=5

pone-0057976-g004: OMAG cross-sectional structural images of the mouse pinna in vivo at several time points.(A) The image size is 1.2 mm×1.2 mm. A, auricular cartilage, E, Epidermis. The epidermal layer increased in thickness and migrated together to from a closure, but the auricular cartilage never fully recovered at 60 days post-injury. (B) Quantification of relative change in epidermal thickness over time.
Mentions: Structure images of a cross-section of the pinna obtained with the OCT system is shown in Figure 4. The location of the epidermis (E) and auricular cartilage (A) is indicated by corresponding arrows. By day 4 the edges of the wound are raised compared with the control image. The epidermal thickness (Fig.4B) in the structural images drastically increases at day 4 and gradually subsides thereafter, although never returning to the pre-injury thickness within the timeframe investigated (60 days in this study). To determine the behavior of blood vessel development and structural wound closure during wound healing, a series of OMAG images (Figs. 5 and 6) were obtained on the same animal before and at 30 min and 4, 8, 12, 18, 22, 26, 32, 46 and 60 days after the biopsy punch was performed on the mouse pinna. The branches of an adjacent artery and vein around the wound became gradually thicker starting from day 4 up to day 26 (blue arrow in Figure 5) suggesting a hyperemic response to the tissue surrounding ischemic area.

Bottom Line: The highest rate of wound closure occurred between days 8 and 22.The vessel tortuosity increased during this time suggesting angiogenesis.The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.

ABSTRACT
Optical microangiography (OMAG) and Doppler optical microangiography (DOMAG) are two non-invasive techniques capable of determining the tissue microstructural content, microvasculature angiography, and blood flow velocity and direction. These techniques were used to visualize the acute and chronic microvascular and tissue responses upon an injury in vivo. A tissue wound was induced using a 0.5 mm biopsy punch on a mouse pinna. The changes in the microangiography, blood flow velocity and direction were quantified for the acute (<30 min) wound response and the changes in the tissue structure and microangiography were determined for the chronic wound response (30 min-60 days). The initial wound triggered recruitment of peripheral capillaries, as well as redirection of main arterial and venous blood flow within 3 min. The complex vascular networks and new vessel formation were quantified during the chronic response using fractal dimension. The highest rate of wound closure occurred between days 8 and 22. The vessel tortuosity increased during this time suggesting angiogenesis. Taken together, these data signify that OMAG has the capability to track acute and chronic changes in blood flow, microangiography and structure during wound healing. The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.

Show MeSH
Related in: MedlinePlus