Limits...
Tracking dynamic microvascular changes during healing after complete biopsy punch on the mouse pinna using optical microangiography.

Jung Y, Dziennis S, Zhi Z, Reif R, Zheng Y, Wang RK - PLoS ONE (2013)

Bottom Line: The highest rate of wound closure occurred between days 8 and 22.The vessel tortuosity increased during this time suggesting angiogenesis.The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.

ABSTRACT
Optical microangiography (OMAG) and Doppler optical microangiography (DOMAG) are two non-invasive techniques capable of determining the tissue microstructural content, microvasculature angiography, and blood flow velocity and direction. These techniques were used to visualize the acute and chronic microvascular and tissue responses upon an injury in vivo. A tissue wound was induced using a 0.5 mm biopsy punch on a mouse pinna. The changes in the microangiography, blood flow velocity and direction were quantified for the acute (<30 min) wound response and the changes in the tissue structure and microangiography were determined for the chronic wound response (30 min-60 days). The initial wound triggered recruitment of peripheral capillaries, as well as redirection of main arterial and venous blood flow within 3 min. The complex vascular networks and new vessel formation were quantified during the chronic response using fractal dimension. The highest rate of wound closure occurred between days 8 and 22. The vessel tortuosity increased during this time suggesting angiogenesis. Taken together, these data signify that OMAG has the capability to track acute and chronic changes in blood flow, microangiography and structure during wound healing. The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.

Show MeSH

Related in: MedlinePlus

Capillary recruitment of peripheral vessels at several time points after the wound.(A) White arrow indicates the large vessels that disappear, and the red arrows indicate the capillary vessels that are recruited after the wound. (B) Relative change in vessel area density in response to biopsy punch.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3585416&req=5

pone-0057976-g003: Capillary recruitment of peripheral vessels at several time points after the wound.(A) White arrow indicates the large vessels that disappear, and the red arrows indicate the capillary vessels that are recruited after the wound. (B) Relative change in vessel area density in response to biopsy punch.

Mentions: To understand the acute blood vessel responses after the biopsy punch, OMAG images were captured at 5, 10, 20 and 30 minutes after induction of the wound (Figure 3). The wound was located in the upper right hand corner of the field of view. This can be visualized by the loss of blood flow and thus disappearance of the vessel depicted by the white arrow. Blood flow in pre-existing capillaries was immediately recruited after initiation of the wound (red arrows), and its domino effect could be observed as other capillary branches appeared at 10 min. By 20 min more vessels are detected compared with 10 min indicating increased compensatory blood flow (red arrows). To quantify the capillary recruitment, the relative vessel area density change was obtained as described by Reif et al. [31]. The vessel area density increased by 5 min and peaked at 20 min after injury, suggesting an increase in the number of capillaries present after the injury.


Tracking dynamic microvascular changes during healing after complete biopsy punch on the mouse pinna using optical microangiography.

Jung Y, Dziennis S, Zhi Z, Reif R, Zheng Y, Wang RK - PLoS ONE (2013)

Capillary recruitment of peripheral vessels at several time points after the wound.(A) White arrow indicates the large vessels that disappear, and the red arrows indicate the capillary vessels that are recruited after the wound. (B) Relative change in vessel area density in response to biopsy punch.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585416&req=5

pone-0057976-g003: Capillary recruitment of peripheral vessels at several time points after the wound.(A) White arrow indicates the large vessels that disappear, and the red arrows indicate the capillary vessels that are recruited after the wound. (B) Relative change in vessel area density in response to biopsy punch.
Mentions: To understand the acute blood vessel responses after the biopsy punch, OMAG images were captured at 5, 10, 20 and 30 minutes after induction of the wound (Figure 3). The wound was located in the upper right hand corner of the field of view. This can be visualized by the loss of blood flow and thus disappearance of the vessel depicted by the white arrow. Blood flow in pre-existing capillaries was immediately recruited after initiation of the wound (red arrows), and its domino effect could be observed as other capillary branches appeared at 10 min. By 20 min more vessels are detected compared with 10 min indicating increased compensatory blood flow (red arrows). To quantify the capillary recruitment, the relative vessel area density change was obtained as described by Reif et al. [31]. The vessel area density increased by 5 min and peaked at 20 min after injury, suggesting an increase in the number of capillaries present after the injury.

Bottom Line: The highest rate of wound closure occurred between days 8 and 22.The vessel tortuosity increased during this time suggesting angiogenesis.The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, University of Washington, Seattle, Washington, United States of America.

ABSTRACT
Optical microangiography (OMAG) and Doppler optical microangiography (DOMAG) are two non-invasive techniques capable of determining the tissue microstructural content, microvasculature angiography, and blood flow velocity and direction. These techniques were used to visualize the acute and chronic microvascular and tissue responses upon an injury in vivo. A tissue wound was induced using a 0.5 mm biopsy punch on a mouse pinna. The changes in the microangiography, blood flow velocity and direction were quantified for the acute (<30 min) wound response and the changes in the tissue structure and microangiography were determined for the chronic wound response (30 min-60 days). The initial wound triggered recruitment of peripheral capillaries, as well as redirection of main arterial and venous blood flow within 3 min. The complex vascular networks and new vessel formation were quantified during the chronic response using fractal dimension. The highest rate of wound closure occurred between days 8 and 22. The vessel tortuosity increased during this time suggesting angiogenesis. Taken together, these data signify that OMAG has the capability to track acute and chronic changes in blood flow, microangiography and structure during wound healing. The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.

Show MeSH
Related in: MedlinePlus