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Dynamic modeling of cell migration and spreading behaviors on fibronectin coated planar substrates and micropatterned geometries.

Kim MC, Neal DM, Kamm RD, Asada HH - PLoS Comput. Biol. (2013)

Bottom Line: The integrative model of this paper successfully reproduced these experimental results and indicates the mechanism of cell migration and spreading.In addition, the integrated model is validated by showing persistent high stress concentrations at sharp geometrically patterned edges.This model will be used as a predictive model to assist in design and data processing of upcoming microfluidic cell migration assays.

View Article: PubMed Central - PubMed

Affiliation: BioSystem & Micromechanics IRG, Singapore MIT Alliance Research Technology, Singapore. mincheol@mit.edu

ABSTRACT
An integrative cell migration model incorporating focal adhesion (FA) dynamics, cytoskeleton and nucleus remodeling, actin motor activity, and lamellipodia protrusion is developed for predicting cell spreading and migration behaviors. This work is motivated by two experimental works: (1) cell migration on 2-D substrates under various fibronectin concentrations and (2) cell spreading on 2-D micropatterned geometries. These works suggest (1) cell migration speed takes a maximum at a particular ligand density (∼1140 molecules/µm(2)) and (2) that strong traction forces at the corners of the patterns may exist due to combined effects exerted by actin stress fibers (SFs). The integrative model of this paper successfully reproduced these experimental results and indicates the mechanism of cell migration and spreading. In this paper, the mechanical structure of the cell is modeled as having two elastic membranes: an outer cell membrane and an inner nuclear membrane. The two elastic membranes are connected by SFs, which are extended from focal adhesions on the cortical surface to the nuclear membrane. In addition, the model also includes ventral SFs bridging two focal adhesions on the cell surface. The cell deforms and gains traction as transmembrane integrins distributed over the outer cell membrane bond to ligands on the ECM surface, activate SFs, and form focal adhesions. The relationship between the cell migration speed and fibronectin concentration agrees with existing experimental data for Chinese hamster ovary (CHO) cell migrations on fibronectin coated surfaces. In addition, the integrated model is validated by showing persistent high stress concentrations at sharp geometrically patterned edges. This model will be used as a predictive model to assist in design and data processing of upcoming microfluidic cell migration assays.

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Dynamic model of cell migration.A) Integrated cell migration model consisting of the cytoskeleton, the nucleus, N integrin nodes on the surface of cytoskeleton, N nuclear nodes on the surface of nucleus, and two types of actin SFs which connect the integrin node to the nuclear node and between integrin nodes; a top view of the model showing triangular mesh network of double membranes of cytoskeleton and nucleus. B) the free body diagram of the i-th integrin node in the circle marked in A) where five external forces are acting. Note that, while shown in 2-D, the force balance exists in 3-D.
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pcbi-1002926-g001: Dynamic model of cell migration.A) Integrated cell migration model consisting of the cytoskeleton, the nucleus, N integrin nodes on the surface of cytoskeleton, N nuclear nodes on the surface of nucleus, and two types of actin SFs which connect the integrin node to the nuclear node and between integrin nodes; a top view of the model showing triangular mesh network of double membranes of cytoskeleton and nucleus. B) the free body diagram of the i-th integrin node in the circle marked in A) where five external forces are acting. Note that, while shown in 2-D, the force balance exists in 3-D.

Mentions: We model the geometric structure of a cell as a double mesh structure: the outer mesh representing the cell membrane and the inner mesh for the nucleus membrane. See Figure 1-A. Each mesh consists of N nodes connected elastically to adjacent nodes, forming a double elastic membrane. The inner and outer mesh nodes may be connected when SFs are formed between the nucleus and the cell membrane [30], [31]. Multiple transmembrane integrins are bundled together and placed at each node on the outer mesh. They can bind to ligands on the matrix substrate, forming a focal adhesion, to which a SF is connected (Figure 2-A). Furthermore, the model also includes ventral SFs which extend between two focal adhesions.


Dynamic modeling of cell migration and spreading behaviors on fibronectin coated planar substrates and micropatterned geometries.

Kim MC, Neal DM, Kamm RD, Asada HH - PLoS Comput. Biol. (2013)

Dynamic model of cell migration.A) Integrated cell migration model consisting of the cytoskeleton, the nucleus, N integrin nodes on the surface of cytoskeleton, N nuclear nodes on the surface of nucleus, and two types of actin SFs which connect the integrin node to the nuclear node and between integrin nodes; a top view of the model showing triangular mesh network of double membranes of cytoskeleton and nucleus. B) the free body diagram of the i-th integrin node in the circle marked in A) where five external forces are acting. Note that, while shown in 2-D, the force balance exists in 3-D.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3585413&req=5

pcbi-1002926-g001: Dynamic model of cell migration.A) Integrated cell migration model consisting of the cytoskeleton, the nucleus, N integrin nodes on the surface of cytoskeleton, N nuclear nodes on the surface of nucleus, and two types of actin SFs which connect the integrin node to the nuclear node and between integrin nodes; a top view of the model showing triangular mesh network of double membranes of cytoskeleton and nucleus. B) the free body diagram of the i-th integrin node in the circle marked in A) where five external forces are acting. Note that, while shown in 2-D, the force balance exists in 3-D.
Mentions: We model the geometric structure of a cell as a double mesh structure: the outer mesh representing the cell membrane and the inner mesh for the nucleus membrane. See Figure 1-A. Each mesh consists of N nodes connected elastically to adjacent nodes, forming a double elastic membrane. The inner and outer mesh nodes may be connected when SFs are formed between the nucleus and the cell membrane [30], [31]. Multiple transmembrane integrins are bundled together and placed at each node on the outer mesh. They can bind to ligands on the matrix substrate, forming a focal adhesion, to which a SF is connected (Figure 2-A). Furthermore, the model also includes ventral SFs which extend between two focal adhesions.

Bottom Line: The integrative model of this paper successfully reproduced these experimental results and indicates the mechanism of cell migration and spreading.In addition, the integrated model is validated by showing persistent high stress concentrations at sharp geometrically patterned edges.This model will be used as a predictive model to assist in design and data processing of upcoming microfluidic cell migration assays.

View Article: PubMed Central - PubMed

Affiliation: BioSystem & Micromechanics IRG, Singapore MIT Alliance Research Technology, Singapore. mincheol@mit.edu

ABSTRACT
An integrative cell migration model incorporating focal adhesion (FA) dynamics, cytoskeleton and nucleus remodeling, actin motor activity, and lamellipodia protrusion is developed for predicting cell spreading and migration behaviors. This work is motivated by two experimental works: (1) cell migration on 2-D substrates under various fibronectin concentrations and (2) cell spreading on 2-D micropatterned geometries. These works suggest (1) cell migration speed takes a maximum at a particular ligand density (∼1140 molecules/µm(2)) and (2) that strong traction forces at the corners of the patterns may exist due to combined effects exerted by actin stress fibers (SFs). The integrative model of this paper successfully reproduced these experimental results and indicates the mechanism of cell migration and spreading. In this paper, the mechanical structure of the cell is modeled as having two elastic membranes: an outer cell membrane and an inner nuclear membrane. The two elastic membranes are connected by SFs, which are extended from focal adhesions on the cortical surface to the nuclear membrane. In addition, the model also includes ventral SFs bridging two focal adhesions on the cell surface. The cell deforms and gains traction as transmembrane integrins distributed over the outer cell membrane bond to ligands on the ECM surface, activate SFs, and form focal adhesions. The relationship between the cell migration speed and fibronectin concentration agrees with existing experimental data for Chinese hamster ovary (CHO) cell migrations on fibronectin coated surfaces. In addition, the integrated model is validated by showing persistent high stress concentrations at sharp geometrically patterned edges. This model will be used as a predictive model to assist in design and data processing of upcoming microfluidic cell migration assays.

Show MeSH
Related in: MedlinePlus