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Transcutaneous electrical nerve stimulation (TENS) improves the diabetic cytopathy (DCP) via up-regulation of CGRP and cAMP.

Ding L, Song T, Yi C, Huang Y, Yu W, Ling L, Dai Y, Wei Z - PLoS ONE (2013)

Bottom Line: The expression of calcitonin gene-related peptide (CGRP) was analyzed by RT-PCR and Western blotting.Furthermore, TENS significantly increased bladder wet weight, volume threshold for micturition and reduced PVR, V% and cAMP content of the bladder.TENS also significantly up-regulated the cAMP content in the bladder body and base compared with diabetic rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ABSTRACT
The objective of this study was to investigate the effects and mechanism of Transcutaneous Electrical Nerve Stimulation (TENS) on the diabetic cytopathy (DCP) in the diabetic bladder. A total of 45 rats were randomly divided into diabetes mellitus (DM)/TENS group (n=15), DM group (n=15) and control group (n=15). The rats in the DM/TENS and TENS groups were electronically stimulated (stimulating parameters: intensity-31 V, frequency-31 Hz, and duration of stimulation of 15 min) for three weeks. Bladder histology, urodynamics and contractile responses to field stimulation and carbachol were determined. The expression of calcitonin gene-related peptide (CGRP) was analyzed by RT-PCR and Western blotting. The results showed that contractile responses of the DM rats were ameliorated after 3 weeks of TENS. Furthermore, TENS significantly increased bladder wet weight, volume threshold for micturition and reduced PVR, V% and cAMP content of the bladder. The mRNA and protein levels of CGRP in dorsal root ganglion (DRG) in the DM/TENS group were higher than those in the DM group. TENS also significantly up-regulated the cAMP content in the bladder body and base compared with diabetic rats. We conclude that TENS can significantly improve the urine contractility and ameliorate the feeling of bladder fullness in DM rats possibly via up-regulation of cAMP and CGRP in DRG.

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Frequency-tension curve of electrical field stimulation (**P<0.01 VS control group, *P<0.05 VS control group,▴▴P<0.01 VS DM/TENS group,▴P<0.05 VS DM/TENS group).
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pone-0057477-g003: Frequency-tension curve of electrical field stimulation (**P<0.01 VS control group, *P<0.05 VS control group,▴▴P<0.01 VS DM/TENS group,▴P<0.05 VS DM/TENS group).

Mentions: The peak force in response to ACh increased in the bladder strips as the concentration of ACh increased. The bladder strips from the diabetic rats produced more force than the same size strips from the controls. The sigmoidal concentration-response curve was significantly shifted upward in the bladder strips of the DM group. TENS reduced the contractile force mildly in the diabetic bladder strips (Figure 2). The peak force in response to EFS increased in the bladder strips as the frequency of stimulation increased. Although the bladder strips from the diabetic rats produced more force in absolute terms than the same size strips from the control rats, the diabetic bladder strips (per 100 mg) actually produced less force at the different frequencies in terms of each strip weight than the control strips. The frequency-response curve was significantly shifted downward in the bladder strips of DM group. TENS significantly potentiated the neurogenic contraction in the diabetic bladder (Figure 3).


Transcutaneous electrical nerve stimulation (TENS) improves the diabetic cytopathy (DCP) via up-regulation of CGRP and cAMP.

Ding L, Song T, Yi C, Huang Y, Yu W, Ling L, Dai Y, Wei Z - PLoS ONE (2013)

Frequency-tension curve of electrical field stimulation (**P<0.01 VS control group, *P<0.05 VS control group,▴▴P<0.01 VS DM/TENS group,▴P<0.05 VS DM/TENS group).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585412&req=5

pone-0057477-g003: Frequency-tension curve of electrical field stimulation (**P<0.01 VS control group, *P<0.05 VS control group,▴▴P<0.01 VS DM/TENS group,▴P<0.05 VS DM/TENS group).
Mentions: The peak force in response to ACh increased in the bladder strips as the concentration of ACh increased. The bladder strips from the diabetic rats produced more force than the same size strips from the controls. The sigmoidal concentration-response curve was significantly shifted upward in the bladder strips of the DM group. TENS reduced the contractile force mildly in the diabetic bladder strips (Figure 2). The peak force in response to EFS increased in the bladder strips as the frequency of stimulation increased. Although the bladder strips from the diabetic rats produced more force in absolute terms than the same size strips from the control rats, the diabetic bladder strips (per 100 mg) actually produced less force at the different frequencies in terms of each strip weight than the control strips. The frequency-response curve was significantly shifted downward in the bladder strips of DM group. TENS significantly potentiated the neurogenic contraction in the diabetic bladder (Figure 3).

Bottom Line: The expression of calcitonin gene-related peptide (CGRP) was analyzed by RT-PCR and Western blotting.Furthermore, TENS significantly increased bladder wet weight, volume threshold for micturition and reduced PVR, V% and cAMP content of the bladder.TENS also significantly up-regulated the cAMP content in the bladder body and base compared with diabetic rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ABSTRACT
The objective of this study was to investigate the effects and mechanism of Transcutaneous Electrical Nerve Stimulation (TENS) on the diabetic cytopathy (DCP) in the diabetic bladder. A total of 45 rats were randomly divided into diabetes mellitus (DM)/TENS group (n=15), DM group (n=15) and control group (n=15). The rats in the DM/TENS and TENS groups were electronically stimulated (stimulating parameters: intensity-31 V, frequency-31 Hz, and duration of stimulation of 15 min) for three weeks. Bladder histology, urodynamics and contractile responses to field stimulation and carbachol were determined. The expression of calcitonin gene-related peptide (CGRP) was analyzed by RT-PCR and Western blotting. The results showed that contractile responses of the DM rats were ameliorated after 3 weeks of TENS. Furthermore, TENS significantly increased bladder wet weight, volume threshold for micturition and reduced PVR, V% and cAMP content of the bladder. The mRNA and protein levels of CGRP in dorsal root ganglion (DRG) in the DM/TENS group were higher than those in the DM group. TENS also significantly up-regulated the cAMP content in the bladder body and base compared with diabetic rats. We conclude that TENS can significantly improve the urine contractility and ameliorate the feeling of bladder fullness in DM rats possibly via up-regulation of cAMP and CGRP in DRG.

Show MeSH
Related in: MedlinePlus