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The novel approach to enhance seed security: dual anti-counterfeiting methods applied on tobacco pelleted seeds.

Guan Y, Wang J, Tian Y, Hu W, Zhu L, Zhu S, Hu J - PLoS ONE (2013)

Bottom Line: Fluorescein (FR), rhodamine B (RB), and magnetic powder (MP) were used as anti-counterfeiting labels.In addition, the cotyledon tip of seedlings treated with FR and MP together represented bright green fluorescence under illumination of blue light (478 nm).And the seedling cotyledon vein treated with RB and MP together showed red fluorescence under green light (546 nm).

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China.

ABSTRACT
Seed security is of prime importance for agriculture. To protect true seeds from being faked, more secure dual anti-counterfeiting technologies for tobacco (Nicotiana tabacum L.) pelleted seed were developed in this paper. Fluorescein (FR), rhodamine B (RB), and magnetic powder (MP) were used as anti-counterfeiting labels. According to their different properties and the special seed pelleting process, four dual-labeling treatments were conducted for two tobacco varieties, MS Yunyan85 (MSYY85) and Honghua Dajinyuan (HHDJY). Then the seed germination and seedling growth status were investigated, and the fluorescence in cracked pellets and developing seedlings was observed under different excitation lights. The results showed that FR, RB, and MP had no negative effects on the germination, seedling growth, and MDA content of the pelleted seeds, and even some treatments significantly enhanced seedling dry weight, vigor index, and shoot height in MS YY85, and increased SOD activity and chlorophyll content in HHDJY as compared to the control. In addition, the cotyledon tip of seedlings treated with FR and MP together represented bright green fluorescence under illumination of blue light (478 nm). And the seedling cotyledon vein treated with RB and MP together showed red fluorescence under green light (546 nm). All seeds pelleted with magnetic powder of proper concentration could be attracted by a magnet. Thus, it indicated that those new dual-labeling methods that fluorescent compound and magnetic powder simultaneously applied in the same seed pellets definitely improved anti-counterfeiting technology and enhanced the seed security. This technology will ensure that high quality seed will be used in the crop production.

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Fluorescence in seedling of MSYY85 pelleted with T3 method under illumination of different lights.Ck: the top row, seedlings from control seeds pelleted without rhodamine B and magnetic powder after 7 days germination (×7); two columns from left to right: under natural light and green light, respectively; A and B: seedlings from seeds pelleted with T3 (2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.8 g talc and 4.2 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels)) germinated for 7 days (A, the second row) and 16 days (B, the third row), respectively (×7); a: the cotyledon of control seedling under green light excitation (546 nm) (×20); b and c: the cotyledon of T3 seedling under green light excitation (546 nm) after 7 and 16 days germination, respectively (×20).
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pone-0057274-g005: Fluorescence in seedling of MSYY85 pelleted with T3 method under illumination of different lights.Ck: the top row, seedlings from control seeds pelleted without rhodamine B and magnetic powder after 7 days germination (×7); two columns from left to right: under natural light and green light, respectively; A and B: seedlings from seeds pelleted with T3 (2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.8 g talc and 4.2 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels)) germinated for 7 days (A, the second row) and 16 days (B, the third row), respectively (×7); a: the cotyledon of control seedling under green light excitation (546 nm) (×20); b and c: the cotyledon of T3 seedling under green light excitation (546 nm) after 7 and 16 days germination, respectively (×20).

Mentions: After treated with T3, the cotyledons vein of 7-day-old and even 16-day-old seedlings showed obviously red fluorescence when excitation by green light (Figure 5b and 5c). However this phenomenon could not be found in the seedling of the control (Figure 5a). The T4 had the similar results to the T3 (data not shown).


The novel approach to enhance seed security: dual anti-counterfeiting methods applied on tobacco pelleted seeds.

Guan Y, Wang J, Tian Y, Hu W, Zhu L, Zhu S, Hu J - PLoS ONE (2013)

Fluorescence in seedling of MSYY85 pelleted with T3 method under illumination of different lights.Ck: the top row, seedlings from control seeds pelleted without rhodamine B and magnetic powder after 7 days germination (×7); two columns from left to right: under natural light and green light, respectively; A and B: seedlings from seeds pelleted with T3 (2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.8 g talc and 4.2 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels)) germinated for 7 days (A, the second row) and 16 days (B, the third row), respectively (×7); a: the cotyledon of control seedling under green light excitation (546 nm) (×20); b and c: the cotyledon of T3 seedling under green light excitation (546 nm) after 7 and 16 days germination, respectively (×20).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585396&req=5

pone-0057274-g005: Fluorescence in seedling of MSYY85 pelleted with T3 method under illumination of different lights.Ck: the top row, seedlings from control seeds pelleted without rhodamine B and magnetic powder after 7 days germination (×7); two columns from left to right: under natural light and green light, respectively; A and B: seedlings from seeds pelleted with T3 (2 g seeds pelleted with 15 g bentonite and 84 g powder mixture which consisted of 79.8 g talc and 4.2 g magnetic powder. Meanwhile, a 2.0 mg/ml of rhodamine B solution was sprayed in place of water when the seed was coated with bentonite (RB and MP dual-labels)) germinated for 7 days (A, the second row) and 16 days (B, the third row), respectively (×7); a: the cotyledon of control seedling under green light excitation (546 nm) (×20); b and c: the cotyledon of T3 seedling under green light excitation (546 nm) after 7 and 16 days germination, respectively (×20).
Mentions: After treated with T3, the cotyledons vein of 7-day-old and even 16-day-old seedlings showed obviously red fluorescence when excitation by green light (Figure 5b and 5c). However this phenomenon could not be found in the seedling of the control (Figure 5a). The T4 had the similar results to the T3 (data not shown).

Bottom Line: Fluorescein (FR), rhodamine B (RB), and magnetic powder (MP) were used as anti-counterfeiting labels.In addition, the cotyledon tip of seedlings treated with FR and MP together represented bright green fluorescence under illumination of blue light (478 nm).And the seedling cotyledon vein treated with RB and MP together showed red fluorescence under green light (546 nm).

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China.

ABSTRACT
Seed security is of prime importance for agriculture. To protect true seeds from being faked, more secure dual anti-counterfeiting technologies for tobacco (Nicotiana tabacum L.) pelleted seed were developed in this paper. Fluorescein (FR), rhodamine B (RB), and magnetic powder (MP) were used as anti-counterfeiting labels. According to their different properties and the special seed pelleting process, four dual-labeling treatments were conducted for two tobacco varieties, MS Yunyan85 (MSYY85) and Honghua Dajinyuan (HHDJY). Then the seed germination and seedling growth status were investigated, and the fluorescence in cracked pellets and developing seedlings was observed under different excitation lights. The results showed that FR, RB, and MP had no negative effects on the germination, seedling growth, and MDA content of the pelleted seeds, and even some treatments significantly enhanced seedling dry weight, vigor index, and shoot height in MS YY85, and increased SOD activity and chlorophyll content in HHDJY as compared to the control. In addition, the cotyledon tip of seedlings treated with FR and MP together represented bright green fluorescence under illumination of blue light (478 nm). And the seedling cotyledon vein treated with RB and MP together showed red fluorescence under green light (546 nm). All seeds pelleted with magnetic powder of proper concentration could be attracted by a magnet. Thus, it indicated that those new dual-labeling methods that fluorescent compound and magnetic powder simultaneously applied in the same seed pellets definitely improved anti-counterfeiting technology and enhanced the seed security. This technology will ensure that high quality seed will be used in the crop production.

Show MeSH
Related in: MedlinePlus