Limits...
The novel approach to enhance seed security: dual anti-counterfeiting methods applied on tobacco pelleted seeds.

Guan Y, Wang J, Tian Y, Hu W, Zhu L, Zhu S, Hu J - PLoS ONE (2013)

Bottom Line: Fluorescein (FR), rhodamine B (RB), and magnetic powder (MP) were used as anti-counterfeiting labels.In addition, the cotyledon tip of seedlings treated with FR and MP together represented bright green fluorescence under illumination of blue light (478 nm).And the seedling cotyledon vein treated with RB and MP together showed red fluorescence under green light (546 nm).

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China.

ABSTRACT
Seed security is of prime importance for agriculture. To protect true seeds from being faked, more secure dual anti-counterfeiting technologies for tobacco (Nicotiana tabacum L.) pelleted seed were developed in this paper. Fluorescein (FR), rhodamine B (RB), and magnetic powder (MP) were used as anti-counterfeiting labels. According to their different properties and the special seed pelleting process, four dual-labeling treatments were conducted for two tobacco varieties, MS Yunyan85 (MSYY85) and Honghua Dajinyuan (HHDJY). Then the seed germination and seedling growth status were investigated, and the fluorescence in cracked pellets and developing seedlings was observed under different excitation lights. The results showed that FR, RB, and MP had no negative effects on the germination, seedling growth, and MDA content of the pelleted seeds, and even some treatments significantly enhanced seedling dry weight, vigor index, and shoot height in MS YY85, and increased SOD activity and chlorophyll content in HHDJY as compared to the control. In addition, the cotyledon tip of seedlings treated with FR and MP together represented bright green fluorescence under illumination of blue light (478 nm). And the seedling cotyledon vein treated with RB and MP together showed red fluorescence under green light (546 nm). All seeds pelleted with magnetic powder of proper concentration could be attracted by a magnet. Thus, it indicated that those new dual-labeling methods that fluorescent compound and magnetic powder simultaneously applied in the same seed pellets definitely improved anti-counterfeiting technology and enhanced the seed security. This technology will ensure that high quality seed will be used in the crop production.

Show MeSH
Effects of dual-labeling methods on superoxidase dismutase (SOD) activity and malondialdehyde (MDA) content of tobacco seedling.*significant difference (α = 0.05, LSD) among treatments within the same variety. Error bars represent ±S.E.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3585396&req=5

pone-0057274-g001: Effects of dual-labeling methods on superoxidase dismutase (SOD) activity and malondialdehyde (MDA) content of tobacco seedling.*significant difference (α = 0.05, LSD) among treatments within the same variety. Error bars represent ±S.E.

Mentions: For MSYY85, there were no significant differences in SOD activity and the contents of MDA, chlorophyll a, chlorophyll b, carotenoids and total chlorophyll among the control and the four pelleting treatments (Figure 1 and Figure 2). However for HHDJY, the SOD activity of T2 was significantly higher than that of the control (Figure 1). The MDA contents of four treatments had no significant differences from the control. Meanwhile, after treated with T4, the contents of chlorophyll a, carotenoids and total chlorophyll in seedling were increased significantly compared with the control (Figure 2).


The novel approach to enhance seed security: dual anti-counterfeiting methods applied on tobacco pelleted seeds.

Guan Y, Wang J, Tian Y, Hu W, Zhu L, Zhu S, Hu J - PLoS ONE (2013)

Effects of dual-labeling methods on superoxidase dismutase (SOD) activity and malondialdehyde (MDA) content of tobacco seedling.*significant difference (α = 0.05, LSD) among treatments within the same variety. Error bars represent ±S.E.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585396&req=5

pone-0057274-g001: Effects of dual-labeling methods on superoxidase dismutase (SOD) activity and malondialdehyde (MDA) content of tobacco seedling.*significant difference (α = 0.05, LSD) among treatments within the same variety. Error bars represent ±S.E.
Mentions: For MSYY85, there were no significant differences in SOD activity and the contents of MDA, chlorophyll a, chlorophyll b, carotenoids and total chlorophyll among the control and the four pelleting treatments (Figure 1 and Figure 2). However for HHDJY, the SOD activity of T2 was significantly higher than that of the control (Figure 1). The MDA contents of four treatments had no significant differences from the control. Meanwhile, after treated with T4, the contents of chlorophyll a, carotenoids and total chlorophyll in seedling were increased significantly compared with the control (Figure 2).

Bottom Line: Fluorescein (FR), rhodamine B (RB), and magnetic powder (MP) were used as anti-counterfeiting labels.In addition, the cotyledon tip of seedlings treated with FR and MP together represented bright green fluorescence under illumination of blue light (478 nm).And the seedling cotyledon vein treated with RB and MP together showed red fluorescence under green light (546 nm).

View Article: PubMed Central - PubMed

Affiliation: Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, P.R. China.

ABSTRACT
Seed security is of prime importance for agriculture. To protect true seeds from being faked, more secure dual anti-counterfeiting technologies for tobacco (Nicotiana tabacum L.) pelleted seed were developed in this paper. Fluorescein (FR), rhodamine B (RB), and magnetic powder (MP) were used as anti-counterfeiting labels. According to their different properties and the special seed pelleting process, four dual-labeling treatments were conducted for two tobacco varieties, MS Yunyan85 (MSYY85) and Honghua Dajinyuan (HHDJY). Then the seed germination and seedling growth status were investigated, and the fluorescence in cracked pellets and developing seedlings was observed under different excitation lights. The results showed that FR, RB, and MP had no negative effects on the germination, seedling growth, and MDA content of the pelleted seeds, and even some treatments significantly enhanced seedling dry weight, vigor index, and shoot height in MS YY85, and increased SOD activity and chlorophyll content in HHDJY as compared to the control. In addition, the cotyledon tip of seedlings treated with FR and MP together represented bright green fluorescence under illumination of blue light (478 nm). And the seedling cotyledon vein treated with RB and MP together showed red fluorescence under green light (546 nm). All seeds pelleted with magnetic powder of proper concentration could be attracted by a magnet. Thus, it indicated that those new dual-labeling methods that fluorescent compound and magnetic powder simultaneously applied in the same seed pellets definitely improved anti-counterfeiting technology and enhanced the seed security. This technology will ensure that high quality seed will be used in the crop production.

Show MeSH