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Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) in Litopenaeus vannamei.

Zhang S, Yan H, Li CZ, Chen YH, Yuan FH, Chen YG, Weng SP, He JG - PLoS ONE (2013)

Bottom Line: The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections.The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference.These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G) were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV). Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

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Expression of LvLRRFIP2 mRNA after knockdown by dsRNA-mediated RNAi.Injections of the enhanced EGFP dsRNA and PBS were used as dsRNA controls. Relative expression values were normalized to LvEF1α. The results are based on three independent experiments and expressed as mean values±SD. Statistical significance was calculated by the Student’s t-test (Letters a and b indicate p<0.05 compared with blank (0 h without any treatment) or PBS group, respectively).
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pone-0057456-g006: Expression of LvLRRFIP2 mRNA after knockdown by dsRNA-mediated RNAi.Injections of the enhanced EGFP dsRNA and PBS were used as dsRNA controls. Relative expression values were normalized to LvEF1α. The results are based on three independent experiments and expressed as mean values±SD. Statistical significance was calculated by the Student’s t-test (Letters a and b indicate p<0.05 compared with blank (0 h without any treatment) or PBS group, respectively).

Mentions: DsRNA were used to knockdown all the variants of LvLRRFIP2. The relative expression level of LvLRRFIP2 in hemocytes after dsLvLRRFIP2 interference is shown in Fig. 6. Reduced LvLRRFIP2 mRNA expression was observed at 0.5 d post-injection. The most significant effect was detected 2 d post-injection. The relative expression of LvLRRFIP2 in dsLvLRRFIP2 injected group accounts for 10% of that of blank L. vannamei (0 h without any treatment) and 8% that of the PBS group. Compared with the dsLvLRRFIP2 injection group, the expression of LvLRRFIP2 was not significantly affected by dsEGFP and PBS injection at all the detected times (p>0.05).


Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) in Litopenaeus vannamei.

Zhang S, Yan H, Li CZ, Chen YH, Yuan FH, Chen YG, Weng SP, He JG - PLoS ONE (2013)

Expression of LvLRRFIP2 mRNA after knockdown by dsRNA-mediated RNAi.Injections of the enhanced EGFP dsRNA and PBS were used as dsRNA controls. Relative expression values were normalized to LvEF1α. The results are based on three independent experiments and expressed as mean values±SD. Statistical significance was calculated by the Student’s t-test (Letters a and b indicate p<0.05 compared with blank (0 h without any treatment) or PBS group, respectively).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585381&req=5

pone-0057456-g006: Expression of LvLRRFIP2 mRNA after knockdown by dsRNA-mediated RNAi.Injections of the enhanced EGFP dsRNA and PBS were used as dsRNA controls. Relative expression values were normalized to LvEF1α. The results are based on three independent experiments and expressed as mean values±SD. Statistical significance was calculated by the Student’s t-test (Letters a and b indicate p<0.05 compared with blank (0 h without any treatment) or PBS group, respectively).
Mentions: DsRNA were used to knockdown all the variants of LvLRRFIP2. The relative expression level of LvLRRFIP2 in hemocytes after dsLvLRRFIP2 interference is shown in Fig. 6. Reduced LvLRRFIP2 mRNA expression was observed at 0.5 d post-injection. The most significant effect was detected 2 d post-injection. The relative expression of LvLRRFIP2 in dsLvLRRFIP2 injected group accounts for 10% of that of blank L. vannamei (0 h without any treatment) and 8% that of the PBS group. Compared with the dsLvLRRFIP2 injection group, the expression of LvLRRFIP2 was not significantly affected by dsEGFP and PBS injection at all the detected times (p>0.05).

Bottom Line: The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections.The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference.These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G) were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV). Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

Show MeSH
Related in: MedlinePlus