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Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) in Litopenaeus vannamei.

Zhang S, Yan H, Li CZ, Chen YH, Yuan FH, Chen YG, Weng SP, He JG - PLoS ONE (2013)

Bottom Line: The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections.The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference.These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G) were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV). Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

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Subcellular localizations of LvLRRFIP2s.Drosophila S2 cells were transfected with GFP fusion proteins of pAcLvLRRFIP2s. At 48 h post-transfection, the cells were observed using a Leica laser scanning confocal microscope.
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pone-0057456-g004: Subcellular localizations of LvLRRFIP2s.Drosophila S2 cells were transfected with GFP fusion proteins of pAcLvLRRFIP2s. At 48 h post-transfection, the cells were observed using a Leica laser scanning confocal microscope.

Mentions: The subcellular location of LRRFIP2 has not been previously characterized. LvLRRFIP2s-GFP were observed under confocal microscopy using Drosophila S2 cells to identify the cellular localization of LvLRRFIP2. Although differences exist among the sequences of LvLRRFIP2s, their GFP fusion proteins were all observed in the cytoplasm of S2 cells (Fig. 4), which was consistent with the interaction of LRRFIP2 and MyD88, an adaptor protein downstream of TLRs [8].


Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) in Litopenaeus vannamei.

Zhang S, Yan H, Li CZ, Chen YH, Yuan FH, Chen YG, Weng SP, He JG - PLoS ONE (2013)

Subcellular localizations of LvLRRFIP2s.Drosophila S2 cells were transfected with GFP fusion proteins of pAcLvLRRFIP2s. At 48 h post-transfection, the cells were observed using a Leica laser scanning confocal microscope.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585381&req=5

pone-0057456-g004: Subcellular localizations of LvLRRFIP2s.Drosophila S2 cells were transfected with GFP fusion proteins of pAcLvLRRFIP2s. At 48 h post-transfection, the cells were observed using a Leica laser scanning confocal microscope.
Mentions: The subcellular location of LRRFIP2 has not been previously characterized. LvLRRFIP2s-GFP were observed under confocal microscopy using Drosophila S2 cells to identify the cellular localization of LvLRRFIP2. Although differences exist among the sequences of LvLRRFIP2s, their GFP fusion proteins were all observed in the cytoplasm of S2 cells (Fig. 4), which was consistent with the interaction of LRRFIP2 and MyD88, an adaptor protein downstream of TLRs [8].

Bottom Line: The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections.The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference.These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G) were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV). Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

Show MeSH
Related in: MedlinePlus