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Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) in Litopenaeus vannamei.

Zhang S, Yan H, Li CZ, Chen YH, Yuan FH, Chen YG, Weng SP, He JG - PLoS ONE (2013)

Bottom Line: The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections.The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference.These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G) were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV). Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

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Temporal expression of LvLRRFIP2 in immune-challenged L. vannamei.The relative expression of LvLRRFIP2s in th treated groups ((A) lipope lysaccharide (LPS), (B) poly I:C, (C) CpG-ODN2006, (D) Vibrio parahaemolyticus, (E) Staphyloccocus aureus, (F) white spot syndrome virus (WSSV)) were compared with the control group. The relative expression level of the target genes was normalized to LvEF1α. The results were based on three independent experiments and expressed as mean values ± SD. Statistical significance was calculated using Student’s t-test (*indicates p<0.05 and **indicates p<0.01 compared with the control).
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pone-0057456-g003: Temporal expression of LvLRRFIP2 in immune-challenged L. vannamei.The relative expression of LvLRRFIP2s in th treated groups ((A) lipope lysaccharide (LPS), (B) poly I:C, (C) CpG-ODN2006, (D) Vibrio parahaemolyticus, (E) Staphyloccocus aureus, (F) white spot syndrome virus (WSSV)) were compared with the control group. The relative expression level of the target genes was normalized to LvEF1α. The results were based on three independent experiments and expressed as mean values ± SD. Statistical significance was calculated using Student’s t-test (*indicates p<0.05 and **indicates p<0.01 compared with the control).

Mentions: After LPS challenge, the level of LvLRRFIP2s increased to its peak at 8 h post-injection. After 48 h, the expression of LvLRRFIP2s was not obviously different from the control (Fig. 3A). After challenge with poly I:C, the LvLRRFIP2s expression was upregulated at all the detected times, with the highest expression level at 4 h post-injection (Fig. 3B). The LvLRRFIP2s expression was also upregulated by CpG-ODN2006 challenge (Fig. 3C). LvLRRFIP2s expression has short-term downregulation at 4 h post-injection, but upregulated after V. parahaemolyticus and S. aureus challenge (Fig. 3D, Fig. 3E). Compared with the control group, the WSSV-infected group showed increased LvLRRFIP2s expression starting at 8 h (Fig. 3F).


Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) in Litopenaeus vannamei.

Zhang S, Yan H, Li CZ, Chen YH, Yuan FH, Chen YG, Weng SP, He JG - PLoS ONE (2013)

Temporal expression of LvLRRFIP2 in immune-challenged L. vannamei.The relative expression of LvLRRFIP2s in th treated groups ((A) lipope lysaccharide (LPS), (B) poly I:C, (C) CpG-ODN2006, (D) Vibrio parahaemolyticus, (E) Staphyloccocus aureus, (F) white spot syndrome virus (WSSV)) were compared with the control group. The relative expression level of the target genes was normalized to LvEF1α. The results were based on three independent experiments and expressed as mean values ± SD. Statistical significance was calculated using Student’s t-test (*indicates p<0.05 and **indicates p<0.01 compared with the control).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585381&req=5

pone-0057456-g003: Temporal expression of LvLRRFIP2 in immune-challenged L. vannamei.The relative expression of LvLRRFIP2s in th treated groups ((A) lipope lysaccharide (LPS), (B) poly I:C, (C) CpG-ODN2006, (D) Vibrio parahaemolyticus, (E) Staphyloccocus aureus, (F) white spot syndrome virus (WSSV)) were compared with the control group. The relative expression level of the target genes was normalized to LvEF1α. The results were based on three independent experiments and expressed as mean values ± SD. Statistical significance was calculated using Student’s t-test (*indicates p<0.05 and **indicates p<0.01 compared with the control).
Mentions: After LPS challenge, the level of LvLRRFIP2s increased to its peak at 8 h post-injection. After 48 h, the expression of LvLRRFIP2s was not obviously different from the control (Fig. 3A). After challenge with poly I:C, the LvLRRFIP2s expression was upregulated at all the detected times, with the highest expression level at 4 h post-injection (Fig. 3B). The LvLRRFIP2s expression was also upregulated by CpG-ODN2006 challenge (Fig. 3C). LvLRRFIP2s expression has short-term downregulation at 4 h post-injection, but upregulated after V. parahaemolyticus and S. aureus challenge (Fig. 3D, Fig. 3E). Compared with the control group, the WSSV-infected group showed increased LvLRRFIP2s expression starting at 8 h (Fig. 3F).

Bottom Line: The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections.The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference.These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G) were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV). Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

Show MeSH
Related in: MedlinePlus