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Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) in Litopenaeus vannamei.

Zhang S, Yan H, Li CZ, Chen YH, Yuan FH, Chen YG, Weng SP, He JG - PLoS ONE (2013)

Bottom Line: The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections.The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference.These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G) were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV). Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

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Tissue distributions of LvLRRFIP2s in healthy L. vannamei.Ten animals were used for tissue sampling. LvEF1α was used as the internal control to normalize the cDNA template used for real-time PCR analysis.
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pone-0057456-g002: Tissue distributions of LvLRRFIP2s in healthy L. vannamei.Ten animals were used for tissue sampling. LvEF1α was used as the internal control to normalize the cDNA template used for real-time PCR analysis.

Mentions: The primers LvLRRFIP2-F/LvLRRFIP2-R were designed according to the identical sequences among LvLRRFIP2s and were used to detect their total amount. The expression level of LvLRRFIP2s was highest in the muscle and lowest in hepatopancreas (Fig. 2). The ligands for TLR3 (poly I:C), TLR4 (LPS), TLR9 (CpG-ODN2006), gram-negative bacteria V. parahaemolyticus, gram-positive bacteria S. aureus, and one of the most common and most destructive viral pathogens in shrimp aquaculture, WSSV, were used for the challenge experiments [30], [31]. LvLRRFIP2s was highly expressed in hemocytes, which are important in immune response in L. vannamei. Thus, we selected hemocytes to study LvLRRFIP2s expression in response to immune challenges.


Identification and function of leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) in Litopenaeus vannamei.

Zhang S, Yan H, Li CZ, Chen YH, Yuan FH, Chen YG, Weng SP, He JG - PLoS ONE (2013)

Tissue distributions of LvLRRFIP2s in healthy L. vannamei.Ten animals were used for tissue sampling. LvEF1α was used as the internal control to normalize the cDNA template used for real-time PCR analysis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585381&req=5

pone-0057456-g002: Tissue distributions of LvLRRFIP2s in healthy L. vannamei.Ten animals were used for tissue sampling. LvEF1α was used as the internal control to normalize the cDNA template used for real-time PCR analysis.
Mentions: The primers LvLRRFIP2-F/LvLRRFIP2-R were designed according to the identical sequences among LvLRRFIP2s and were used to detect their total amount. The expression level of LvLRRFIP2s was highest in the muscle and lowest in hepatopancreas (Fig. 2). The ligands for TLR3 (poly I:C), TLR4 (LPS), TLR9 (CpG-ODN2006), gram-negative bacteria V. parahaemolyticus, gram-positive bacteria S. aureus, and one of the most common and most destructive viral pathogens in shrimp aquaculture, WSSV, were used for the challenge experiments [30], [31]. LvLRRFIP2s was highly expressed in hemocytes, which are important in immune response in L. vannamei. Thus, we selected hemocytes to study LvLRRFIP2s expression in response to immune challenges.

Bottom Line: The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections.The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference.These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

View Article: PubMed Central - PubMed

Affiliation: Ministry of Education Key Laboratory of Aquatic Product Safety/State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT
Leucine-rich repeat flightless-I-interacting protein 2 (LRRFIP2) is a myeloid differentiation factor 88-interacting protein with a positive regulatory function in toll-like receptor signaling. In this study, seven LRRFIP2 protein variants (LvLRRFIP2A-G) were identified in Litopenaeus vannamei. All the seven LvLRRFIP2 protein variants encode proteins with a DUF2051 domain. LvLRRFIP2s were upregulated in hemocytes after challenged with lipopolysaccharide, poly I:C, CpG-ODN2006, Vibrio parahaemolyticus, Staphylococcus aureus, and white spot syndrome virus (WSSV). Dual-luciferase reporter assays in Drosophila Schneider 2 cells revealed that LvLRRFIP2 activates the promoters of Drosophila and shrimp AMP genes. The knockdown of LvLRRFIP2 by RNA interference resulted in higher cumulative mortality of L. vannamei upon V. parahaemolyticus but not S. aureus and WSSV infections. The expression of L. vannamei AMP genes were reduced by dsLvLRRFIP2 interference. These results indicate that LvLRRFIP2 has an important function in antibacterials via the regulation of AMP gene expression.

Show MeSH
Related in: MedlinePlus