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Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia.

Totte P, Yaya A, Sery A, Wesonga H, Wade A, Naessens J, Niang M, Thiaucourt F - PLoS ONE (2013)

Bottom Line: A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP).Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment.In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

View Article: PubMed Central - PubMed

Affiliation: Centre International de Recherche en Agronomie pour le Développement, UMR CMAEE, Montpellier, France. philippe.totte@cirad.fr

ABSTRACT
A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4(+) T lymphocytes. Moreover, CD62L expression indicated that both CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

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Related in: MedlinePlus

Cell surface expression of CD62L (a), and CD45RO (b), and CD45R (c) markers by proliferating (R1 gate) and non-proliferationg (R2 gate) CD4+ T cells stimulated with MmmSC Ags.Percentages of positive cells within gated populations are shown on histograms.
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pone-0057509-g005: Cell surface expression of CD62L (a), and CD45RO (b), and CD45R (c) markers by proliferating (R1 gate) and non-proliferationg (R2 gate) CD4+ T cells stimulated with MmmSC Ags.Percentages of positive cells within gated populations are shown on histograms.

Mentions: Analysis of activation/memory markers indicated strong upregulation of CD45RO and down regulation of CD45R surface expression by proliferating CD4+ T lymphocytes (Fig. 5). It can be observed from Fig. 5 that, among proliferating CD4, the majority of CD62L− and CD62L+ also followed that same expression pattern (i.e., upregulation of CD45RO and downregulation of CD45R) which is indicative of a memory phenoytpe.


Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia.

Totte P, Yaya A, Sery A, Wesonga H, Wade A, Naessens J, Niang M, Thiaucourt F - PLoS ONE (2013)

Cell surface expression of CD62L (a), and CD45RO (b), and CD45R (c) markers by proliferating (R1 gate) and non-proliferationg (R2 gate) CD4+ T cells stimulated with MmmSC Ags.Percentages of positive cells within gated populations are shown on histograms.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585371&req=5

pone-0057509-g005: Cell surface expression of CD62L (a), and CD45RO (b), and CD45R (c) markers by proliferating (R1 gate) and non-proliferationg (R2 gate) CD4+ T cells stimulated with MmmSC Ags.Percentages of positive cells within gated populations are shown on histograms.
Mentions: Analysis of activation/memory markers indicated strong upregulation of CD45RO and down regulation of CD45R surface expression by proliferating CD4+ T lymphocytes (Fig. 5). It can be observed from Fig. 5 that, among proliferating CD4, the majority of CD62L− and CD62L+ also followed that same expression pattern (i.e., upregulation of CD45RO and downregulation of CD45R) which is indicative of a memory phenoytpe.

Bottom Line: A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP).Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment.In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

View Article: PubMed Central - PubMed

Affiliation: Centre International de Recherche en Agronomie pour le Développement, UMR CMAEE, Montpellier, France. philippe.totte@cirad.fr

ABSTRACT
A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4(+) T lymphocytes. Moreover, CD62L expression indicated that both CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

Show MeSH
Related in: MedlinePlus