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Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia.

Totte P, Yaya A, Sery A, Wesonga H, Wade A, Naessens J, Niang M, Thiaucourt F - PLoS ONE (2013)

Bottom Line: A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP).Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment.In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

View Article: PubMed Central - PubMed

Affiliation: Centre International de Recherche en Agronomie pour le Développement, UMR CMAEE, Montpellier, France. philippe.totte@cirad.fr

ABSTRACT
A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4(+) T lymphocytes. Moreover, CD62L expression indicated that both CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

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Related in: MedlinePlus

Both CD62L+CD4+ and CD62L−CD4+ T cells proliferate in response to MmmSC stimulation in vitro.Results of a typical four-color flow cytometric analysis are shown for one vaccinated animal. Cells were loaded with CFSE and incubated for 9 days with inactivated MmmSC before cell surface staining and analysis by flow cytometry as follows: (a) a first gate (P1) was used to exclude dead cells (7AAD+) and cell debris (FSC<50); (b) a second gate (P2) was set, among P1 gated cells, to delineate viable and proliferating CD4+ T cells (CFSElow or CFSE<1×103); and (c), histograms were opened on gate P2 to obtain the percentage of CD62L+ cells among proliferating CD4+ T cells (i.e., P3).
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pone-0057509-g004: Both CD62L+CD4+ and CD62L−CD4+ T cells proliferate in response to MmmSC stimulation in vitro.Results of a typical four-color flow cytometric analysis are shown for one vaccinated animal. Cells were loaded with CFSE and incubated for 9 days with inactivated MmmSC before cell surface staining and analysis by flow cytometry as follows: (a) a first gate (P1) was used to exclude dead cells (7AAD+) and cell debris (FSC<50); (b) a second gate (P2) was set, among P1 gated cells, to delineate viable and proliferating CD4+ T cells (CFSElow or CFSE<1×103); and (c), histograms were opened on gate P2 to obtain the percentage of CD62L+ cells among proliferating CD4+ T cells (i.e., P3).

Mentions: We have shown previously that expression of CD62L among proliferating CD4+ T lymphocytes could discriminate between Tem and Tcm in MmmSC presensitized animals [13]. Here, we have assessed the contribution of both populations in anamnestic T-cell responses of vaccinated cattle, focusing on animals that responded above nonvaccinated animals (i.e., 4 out of 5), to concentrate on vaccine-induced T-cell responses. We found that viable CD4+ T lymphocytes actively proliferating in response to MmmSC comprised both CD62L+ and CD62L− populations (Fig. 4). In general, the percentage of CD62L+ cells slightly exceeded the percentage of CD62L− cells among proliferating CD4+ T lymphocytes (Table 1) whereas in non stimulated CD4 the ratio was 45±5%.


Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia.

Totte P, Yaya A, Sery A, Wesonga H, Wade A, Naessens J, Niang M, Thiaucourt F - PLoS ONE (2013)

Both CD62L+CD4+ and CD62L−CD4+ T cells proliferate in response to MmmSC stimulation in vitro.Results of a typical four-color flow cytometric analysis are shown for one vaccinated animal. Cells were loaded with CFSE and incubated for 9 days with inactivated MmmSC before cell surface staining and analysis by flow cytometry as follows: (a) a first gate (P1) was used to exclude dead cells (7AAD+) and cell debris (FSC<50); (b) a second gate (P2) was set, among P1 gated cells, to delineate viable and proliferating CD4+ T cells (CFSElow or CFSE<1×103); and (c), histograms were opened on gate P2 to obtain the percentage of CD62L+ cells among proliferating CD4+ T cells (i.e., P3).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585371&req=5

pone-0057509-g004: Both CD62L+CD4+ and CD62L−CD4+ T cells proliferate in response to MmmSC stimulation in vitro.Results of a typical four-color flow cytometric analysis are shown for one vaccinated animal. Cells were loaded with CFSE and incubated for 9 days with inactivated MmmSC before cell surface staining and analysis by flow cytometry as follows: (a) a first gate (P1) was used to exclude dead cells (7AAD+) and cell debris (FSC<50); (b) a second gate (P2) was set, among P1 gated cells, to delineate viable and proliferating CD4+ T cells (CFSElow or CFSE<1×103); and (c), histograms were opened on gate P2 to obtain the percentage of CD62L+ cells among proliferating CD4+ T cells (i.e., P3).
Mentions: We have shown previously that expression of CD62L among proliferating CD4+ T lymphocytes could discriminate between Tem and Tcm in MmmSC presensitized animals [13]. Here, we have assessed the contribution of both populations in anamnestic T-cell responses of vaccinated cattle, focusing on animals that responded above nonvaccinated animals (i.e., 4 out of 5), to concentrate on vaccine-induced T-cell responses. We found that viable CD4+ T lymphocytes actively proliferating in response to MmmSC comprised both CD62L+ and CD62L− populations (Fig. 4). In general, the percentage of CD62L+ cells slightly exceeded the percentage of CD62L− cells among proliferating CD4+ T lymphocytes (Table 1) whereas in non stimulated CD4 the ratio was 45±5%.

Bottom Line: A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP).Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment.In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

View Article: PubMed Central - PubMed

Affiliation: Centre International de Recherche en Agronomie pour le Développement, UMR CMAEE, Montpellier, France. philippe.totte@cirad.fr

ABSTRACT
A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4(+) T lymphocytes. Moreover, CD62L expression indicated that both CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

Show MeSH
Related in: MedlinePlus